Results 31 to 40 of about 123,037 (250)

Click Detect: A Rapid and Sensitive Assay for Shiga Toxin 2 Detection. [PDF]

Biosensors (Basel)
Shiga toxin–producing Escherichia coli (STEC) are major foodborne pathogens responsible for severe gastrointestinal disease and hemolytic uremic syndrome (HUS). Here we report Click Detect, a novel diagnostic platform that leverages click display – an in vitro protein display technology – to generate uniform and covalently linked protein–cDNA ...
Thomas BM, Webb EL, Yan KL, Fernandez AM, Chen Z.   +4 more
europepmc   +4 more sources

Isogenic Lysogens of Diverse Shiga Toxin 2-Encoding Bacteriophages Produce Markedly Different Amounts of Shiga Toxin [PDF]

Infection and Immunity, 1999
ABSTRACT We produced isogenic Escherichia coli K-12 lysogens of seven different Shiga toxin 2 (Stx2)-encoding bacteriophages derived from clinical Shiga toxin-producing E. coli (STEC) isolates of serotypes O157:H7, O145, O111, and O83 to assess the variability among these phages and
P L, Wagner, D W, Acheson, M K, Waldor
openaire   +2 more sources

Safety and Pharmacokinetics of Chimeric Anti-Shiga Toxin 1 and Anti-Shiga Toxin 2 Monoclonal Antibodies in Healthy Volunteers [PDF]

Antimicrobial Agents and Chemotherapy, 2009
ABSTRACT Shiga toxin (Stx)-producing Escherichia coli (STEC) causes hemorrhagic colitis and hemolytic-uremic syndrome (HUS). The rates of STEC infection and complications, including death, are highest among young children and elderly individuals. There are no causal therapies.
Martin, Bitzan, Ruth, Poole, Mariam, Mehran, Eric, Sicard, Catherine, Brockus, Claire, Thuning-Roberson, Marc, Rivière   +6 more
openaire   +2 more sources

Shiga toxin 1 is more dependent on the P proteins of the ribosomal stalk for depurination activity than Shiga toxin 2 [PDF]

The International Journal of Biochemistry & Cell Biology, 2011
Shiga toxins produced by Escherichia coli O157:H7 are responsible for food poisoning and hemolytic uremic syndrome (HUS). The A subunits of Shiga toxins (Stx1A and Stx2A) inhibit translation by depurinating a specific adenine in the large rRNA. To determine if Stx1A and Stx2A require the ribosomal stalk for depurination, their activity and cytotoxicity
Jia-Chi, Chiou, Xiao-Ping, Li, Miguel, Remacha, Juan P G, Ballesta, Nilgun E, Tumer   +4 more
openaire   +2 more sources

Chemokine Expression in Human Astrocytes in Response to Shiga Toxin 2 [PDF]

International Journal of Inflammation, 2012
Infection with Shiga toxin- (Stx-) producingEscherichia colican lead to hemolytic uremic syndrome (HUS). Approximately, 30% of patients with HUS suffer from complications in the central nervous system (CNS), which is an important determinant of mortality in such patients.
Naomi Kioka, Koichi Minami, Akira Tamura, Norishige Yoshikawa   +3 more
openaire   +3 more sources

Open-source genomic analysis of Shiga-toxin–producing E. coli O104:H4 [PDF]

, 2011
An outbreak caused by Shiga-toxin–producing Escherichia coli O104:H4 occurred in Germany in May and June of 2011, with more than 3000 persons infected. Here, we report a cluster of cases associated with a single family and describe an open-source genomic
Antoine Danchin, Cordesmeyer S, Dongfang Li, Fei Pu, Feng Xi, George F. Gao, Holger Rohde, Huanming Yang, Itoh Y, Jian Wang, Jianguo Xu, Jun Oh, Jun Wang, Junhua Li, Junjie Qin, Kuijper E, Liang Yang, Mark J. Pallen, Martin Aepfelbacher, Martin Christner, Meiru Zhao, Moritz Hentschke, Nicholas J. Loman, Peng Wang, Robin Kobbe, Ruifu Yang, Scheutz F, Sebastian Loos, Shenghui Li, Wentong Chen, Xiangna Zhao, Xianwei Yang, Yajun Song, Yangqing Peng, Yin Li, Yingrui Li, Yuanlin Guan, Yujun Cui, Zhaoxi Zhang, Zhong Cen   +39 more
core   +1 more source

Subtyping of Shiga toxin 2 variants in human-derived Shiga toxin-producingEscherichia colistrains isolated in Japan [PDF]

FEMS Immunology & Medical Microbiology, 2002
Shiga toxin 2 (Stx2) variants have been found to exhibit not only antigenic divergence, but also differences in toxicity for tissue culture cells and animals. To clarify whether all or just a subset of Stx2 variants are important for the virulence of Shiga toxin-producing Escherichia coli, we designed PCR primers to detect and type all reported ...
Hiroshi, Nakao, Kouichi, Kimura, Hiroyuki, Murakami, Tsutomu, Maruyama, Tae, Takeda   +4 more
openaire   +3 more sources

Differential binding of Shiga toxin 2 to human and murine neutrophils [PDF]

Journal of Medical Microbiology, 2007
Shiga toxins (Stx1 and Stx2) are responsible for initiating haemolytic uraemic syndrome, a serious extraintestinal complication caused by enterohaemorrhagicEscherichia coliO157 : H7 infection in humans. Shiga toxins are classical AB5-type exotoxins, consisting of a globotriaosylceramide (Gb3)-binding B subunit pentamer and an enzymic A subunit.
Thomas P, Griener, George L, Mulvey, Paola, Marcato, Glen D, Armstrong   +3 more
openaire   +2 more sources

Comparison of shiga-toxigenic Escherichia coli prevalences among dairy, feedlot, and cow-calf herds in Washington State [PDF]

, 2004
Shiga-toxigenic Escherichia coli (STEC) strains were isolated from 7.4% of 1,440 fecal and farm environmental samples. Shiga toxin gene and STEC prevalences were significantly associated with animal production type and season.
Call, Douglas R., Cobbold, Rowland N., Hancock, Dale D., Rice, Daniel H., Szymanski, Maryanne   +4 more
core   +3 more sources

Shiga toxin 2-induced intestinal pathology in infant rabbits is A-subunit dependent and responsive to the tyrosine kinase and potential ZAK inhibitor imatinib

Frontiers in Cellular and Infection Microbiology, 2012
Shiga toxin producing Escherichia coli (STEC) are a major cause of food-borne illness worldwide. However, a consensus regarding the role Shiga toxins play in the onset of diarrhea and hemorrhagic colitis is lacking.
Samuel eStone, Cheleste eThorpe, Amrita eAhluwalia, Amrita eAhluwalia, Arlin eRogers, Fumiko eObata, Aimee eVozenilek, Glynis eKolling, Anne eKane, Bruce eMagun, Dakshina eJandhyala   +10 more
doaj   +1 more source