Shikimic acid 3-phosphate (S-3-P) is needed as a substrate to detect the enzyme activity of 5-enolpyruvylshikimic acid 3-phosphate synthase (CP4EPSPS), which is expressed in genetically modified soybeans. Therefore, we attempted the over-expression of shikimate kinase II (SK-II) in E. coli to biosynthetically obtain S-3-P.
Hiroshi AKIYAMA +10 more
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Comparative Transcriptome Reveals Benzenoid Biosynthesis Regulation as Inducer of Floral Scent in the Woody Plant Prunus mume [PDF]
Mei (Prunus mume) is a peculiar woody ornamental plant famous for its inviting fragrance in winter. However, in this valuable plant, the mechanism behind floral volatile development remains poorly defined. Therefore, to explore the floral scent formation,
Jie Zhang +6 more
core +1 more source
Abstract Polyploidization has played a key role in plant genome evolution. Eragrostis curvula (Schrad.) Ness, a perennial forage grass species of the Poaceae family, is an excellent model for investigating genome duplication due to its natural variation in ploidy levels.
D. F. Santoro +5 more
wiley +1 more source
Marine pharmacology in 2009-2011: marine compounds with antibacterial, antidiabetic, antifungal, anti-inflammatory, antiprotozoal, antituberculosis, and antiviral activities; affecting the immune and nervous systems, and other miscellaneous mechanisms of action. [PDF]
The peer-reviewed marine pharmacology literature from 2009 to 2011 is presented in this review, following the format used in the 1998–2008 reviews of this series.
A. D. Rodriguez +3 more
core +2 more sources
Gap Analysis of Metabolic Conversions of Off‐Flavors and Antinutrients in Plant‐Based Substrates
ABSTRACT To drastically reduce the carbon footprint of the food production chain, a major shift towards alternatives to conventional meat and dairy products is required. The use of plant‐based proteins is a promising route, but it also comes with challenges: Plant‐based proteins often contain antinutritional factors and off‐flavors, which can ...
Robin I. Kuijpers +16 more
wiley +1 more source
Engineering yeast for high-level production of stilbenoid antioxidants [PDF]
Stilbenoids, including resveratrol and its methylated derivatives, are natural potent antioxidants, produced by some plants in trace amounts as defense compounds.
Borodina, I. +4 more
core +1 more source
Summary In plants, multiple cell types contribute to immunity, but what division of labor exists among cell types when immunity is activated? We compared, at single‐cell resolution, the response of Arabidopsis thaliana leaf cells during pattern‐triggered and effector‐triggered immunity (PTI/ETI), sampled at 3 and 5 h after infection with Pseudomonas ...
Shanshan Wang +5 more
wiley +1 more source
In Vivo Activity, Purification, and Characterization of Shikimate Kinase from Sorghum [PDF]
Sorghum bicolor stems and shoots were observed to phosphorylate exogenously supplied shikimate, forming a product which could not be distinguished from shikimate 3-phosphate by anion exchange and thin layer chromatography. Upon treatment with phosphatase, this product gave rise to a compound which co-chromatographed with shikimate.Shikimate kinase from
J R, Bowen, T, Kosuge
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Transcriptome profiling of grapevine seedless segregants during berry development reveals candidate genes associated with berry weight [PDF]
Indexación: Web of Science; PubMedBackground Berry size is considered as one of the main selection criteria in table grape breeding programs. However, this is a quantitative and polygenic trait, and its genetic determination is still poorly understood ...
A Doligez +90 more
core +3 more sources
Purification and properties of shikimate kinase II from Escherichia coli K-12 [PDF]
Shikimate kinase II was purified to near homogeneity from an Escherichia coli strain which overproduced the enzyme. The apparent Km of this isoenzyme for shikimate was 200 microM, and for ATP it was 160 microM. The Km for shikimate is approximately 100-fold lower than the Km of shikimate kinase I, suggesting that shikimate kinase II is the isoenzyme ...
R C, DeFeyter, J, Pittard
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