Results 31 to 40 of about 22,215 (261)

Sindbis virus replicons and Sindbis virus: assembly of chimeras and of particles deficient in virus RNA [PDF]

Journal of Virology, 1997
Alphaviruses are a well-characterized group of positive-strand RNA viruses. The identification of cis-acting elements in their genomes and their replication strategy have made them useful as vectors for the expression of heterologous genes. In infected cells, the nonstructural proteins, required for replication and transcription of the viral genes, are
I, Frolov, E, Frolova, S, Schlesinger
openaire   +2 more sources

Glycoprotein is enough for sindbis virus-derived DNA vector to express heterogenous genes

Virology Journal, 2011
To investigate the necessity and potential application of structural genes for expressing heterogenous genes from Sindbis virus-derived vector, the DNA-based expression vector pVaXJ was constructed by placing the recombinant genome of sindbis-like virus ...
Fu Juanjuan, Li Jia, Wang Huanqin, Tang Li, Li Jiangjiao, Zhu Wuyang, Liang Guodong   +6 more
doaj   +1 more source

Mutation of CD2AP and SH3KBP1 binding motif in alphavirus nsP3 hypervariable domain results in attenuated virus [PDF]

, 2018
Infection by Chikungunya virus (CHIKV) of the Old World alphaviruses (family Togaviridae) in humans can cause arthritis and arthralgia. The virus encodes four non-structural proteins (nsP) (nsP1, nsp2, nsP3 and nsP4) that act as subunits of the virus ...
Aquilimeba, Muriel, Kasvandik, Sergo, Lulla, Aleksei, Lulla, Valeria, McInerney, Gerald M., Merits, Andres, Morro, Ainhoa Moliner, Mutso, Margit, Saul, Sirle, Smedberg, Cecilia, Tarve, Liisi, Teppor, Mona, Thaa, Bastian, Varjak, Margus   +13 more
core   +3 more sources

Small RNA analysis in Sindbis virus infected human HEK293 cells [PDF]

, 2013
In contrast to the defence mechanism of RNA interference (RNAi) in plants and invertebrates, its role in the innate response to virus infection of mammals is a matter of debate.
A Chakrabarti, A Mortazavi, A Saumet, Andras Donaszi-Ivanov, BC Ho, BR Cullen, BR tenOever, CL Campbell, CM Cirimotich, CW Burke, E Gottwein, EG Strauss, EM Morazzani, ER Mardis, EY Choy, F Ma, F Weber, G Gatto, G Szittya, I Mohorianu, I Mohorianu, IP Greene, Irina Mohorianu, J Fang, JI Henke, JK Ahluwalia, JR Abend, JY Leung, K Prufer, KJ Ishii, KL McKnight, KM Myles, KW Witwer, L Du, M Hariharan, MB Stocks, MC Saleh, ML Metzker, MP Gantier, N Vodovar, P Parameswaran, Penny P. Powell, PV Maillard, RL Pilcher, RL Skalsky, RP Kincaid, RW Williams, S Griffiths-Jones, S Koyama, S Moxon, SW Ding, T Kawai, Tamas Dalmay, V Stollar, W Hou, WB Klimstra, X Lei, Y Kim, Y Li, YQ Wu, Zach N. Adelman, ZN Adelman   +61 more
core   +7 more sources

A novel system for the launch of alphavirus RNA synthesis reveals a role for the Imd pathway in arthropod antiviral response.

PLoS Pathogens, 2009
Alphaviruses are RNA viruses transmitted between vertebrate hosts by arthropod vectors, primarily mosquitoes. How arthropods counteract alphaviruses or viruses per se is not very well understood.
Vasanthi Avadhanula, Brandon P Weasner, Gail G Hardy, Justin P Kumar, Richard W Hardy   +4 more
doaj   +1 more source

Infectious alphavirus production from a simple plasmid transfection+

Virology Journal, 2011
We have developed a new method for producing infectious double subgenomic alphaviruses from plasmids transfected into mammalian cells. A double subgenomic Sindbis virus (TE3'2J) was transcribed from a cytomegalovirus PolII promoter, which results in the ...
Olson Ken E, Lama Siddhi BC, Henderson Brittney R, Steel J Jordan, Geiss Brian J   +4 more
doaj   +1 more source

ZAP's stress granule localization is correlated with its antiviral activity and induced by virus replication. [PDF]

, 2019
Cellular antiviral programs encode molecules capable of targeting multiple steps in the virus lifecycle. Zinc-finger antiviral protein (ZAP) is a central and general regulator of antiviral activity that targets pathogen mRNA stability and translation ...
Jurado, Andrea, Law, Lok Man John, Li, Melody MH, MacDonald, Margaret R, Razooky, Brandon S, Rice, Charles M, You, Shihyun   +6 more
core   +2 more sources

Mitochondrial bioenergetic alterations in mouse neuroblastoma cells infected with Sindbis virus: implications to viral replication and neuronal death.

PLoS ONE, 2012
The metabolic resources crucial for viral replication are provided by the host. Details of the mechanisms by which viruses interact with host metabolism, altering and recruiting high free-energy molecules for their own replication, remain unknown ...
Leandro Silva da Costa, Ana Paula Pereira da Silva, Andrea T Da Poian, Tatiana El-Bacha   +3 more
doaj   +1 more source

Enzymatic Iodination of Sindbis Virus Proteins [PDF]

Journal of Virology, 1973
Sindbis virus was iodinated by using the enzyme lactoperoxidase, an iodination technique which labels only surface proteins. By this technique, the two viral glycoproteins are labeled, and the internal viral protein is not. The two glycoproteins are iodinated to strikingly different extents. This difference in susceptibility to iodination apparently is
B M, Sefton, G G, Wickus, B W, Burge
openaire   +2 more sources

Amino acid substitutions in the E2 glycoprotein of Sindbis-like virus XJ-160 confer the ability to undergo heparan sulfate-dependent infection of mouse embryonic fibroblasts

Virology Journal, 2010
We have recently demonstrated an essential role of the domain of 145-150 amino acid in the E2 glycoprotein of Sindbis virus in the interaction with cellular heparan sulfate (HS) and in the infection of mouse embryonic fibroblasts (MEF) cells.
Zhu Wuyang, Fu Shihong, He Ying, Li Jinping, Liang Guodong   +4 more
doaj   +1 more source