Results 91 to 100 of about 4,724,822 (306)
A systematic overview of single-cell transcriptomics databases, their use cases, and limitations
Frontiers in BioinformaticsRapid advancements in high-throughput single-cell RNA-seq (scRNA-seq) technologies and experimental protocols have led to the generation of vast amounts of transcriptomic data that populates several online databases and repositories.
Mahnoor N. Gondal +12 more
doaj +1 more source
Cell Reports, 2020 Summary: High-dimensional data, such as those generated by single-cell RNA sequencing (scRNA-seq), present challenges in interpretation and visualization.
Cody N. Heiser, Ken S. Lau
doaj +1 more source
, 2022 Despite recent advances in single-cell analysis techniques, the ability of single-cell analysis platforms to track specific cells that secreted cytokines remains limited. Here, we report a microfluidic droplet-based fluorescence imaging platform that can
Wei Shi +17 more
core +1 more source
Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation
FEBS Letters, EarlyView.Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe +3 more
wiley +1 more source
SINGLE CELL METABOLOMICS USING MASS SPECTROMETRY: DEVICES, METHODS AND APPLICATIONS [PDF]
, 2019 Cells are basic functional components of eukaryotic organisms containing rich biological and physiological information. To investigate the nature of cells, a variety of fundamental and mechanistic studies of the cell functions, cellular metabolisms ...
Liu, Renmeng
core
Awesome-Single-Cell: A Crowdsourced Knowledge Resource for Single Cell Data Analysis
, 2019 In parallel with the rapid development of single cell assays has been a similarly unprecedented explosion of analysis approaches, tools, and workflows for interpreting and comprehending the data.
Sean Davis (278224)
core +1 more source
FEBS Letters, EarlyView.This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva +5 more
wiley +1 more source
Perfusion Culture of Mammalian Cells in a Microfluidic Channel with a Built-In Pillar Array
, 2012 In vitro culture of mammalian cells is fundamental to various biological studies such as single cell analysis, pathological research, and drug/therapy development. However, there are limitations with the current in vitro cell culture methods.
Chi Zhang, Zhang, Chi,
core +1 more source
FEBS Letters, EarlyView.The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li +2 more
wiley +1 more source
Single cell analysis of transcriptional activation dynamics.
PLoS ONE, 2010 BackgroundGene activation is thought to occur through a series of temporally defined regulatory steps. However, this process has not been completely evaluated in single living mammalian cells.Methodology/principal findingsTo investigate the timing and ...
Ilona U Rafalska-Metcalf +4 more
doaj +1 more source