Results 21 to 30 of about 5,522,894 (323)
Hierarchical mechanism of amino acid sensing by the T-box riboswitch
Nature Communications, 2018 Riboswitches on 5′ ends of mRNAs are important for bacterial gene regulation. Here the authors probe the mechanism of a tRNA aminoacylation sensing T-box riboswitch using single-molecule fluorescence microscopy to characterize dynamic solution ...
Krishna C. Suddala +5 more
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Label-Free Metabolic Classification of Single Cells in Droplets Using the Phasor Approach to Fluorescence Lifetime Imaging Microscopy. [PDF]
, 2019 Characterization of single cell metabolism is imperative for understanding subcellular functional and biochemical changes associated with healthy tissue development and the progression of numerous diseases.
Aghaamoo, Mohammad +4 more
core +1 more source
scDA: Single cell discriminant analysis for single-cell RNA sequencing data
Computational and Structural Biotechnology Journal, 2021 Single-cell RNA-sequencing (scRNA-seq) techniques provide unprecedented opportunities to investigate phenotypic and molecular heterogeneity in complex biological systems.
Qianqian Shi +4 more
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Single‐cell proteomic analysis
WIREs Mechanisms of Disease, 2020 AbstractThe ability to comprehensively profile proteins in every individual cell of complex biological systems is crucial to advance our understanding of normal physiology and disease pathogenesis. Conventional bulk cell experiments mask the cell heterogeneity in the population, while the single‐cell imaging methods suffer from the limited multiplexing
Thai Pham +3 more
openaire +4 more sources
Single-cell protein analysis [PDF]
Current Opinion in Biotechnology, 2012 Heterogeneity of cellular systems has been widely recognized but only recently have tools become available that allow probing of genes and proteins in single cells to understand it. While the advancement in single cell genomic analysis has been greatly aided by the power of amplification techniques (e.g.
Anup K. Singh, Meiye Wu
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Microfluidic Single-Cell Manipulation and Analysis: Methods and Applications
Micromachines, 2019 In a forest of a hundred thousand trees, no two leaves are alike. Similarly, no two cells in a genetically identical group are the same. This heterogeneity at the single-cell level has been recognized to be vital for the correct interpretation of ...
Tao Luo, Lei Fan, Rong Zhu, Dong Sun
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Molecular Plant-Microbe Interactions, 2004 Resistance and susceptibility in barley to the powdery mildew fungus (Blumeria graminis f. sp. hordei) is determined at the single-cell level. Even in genetically compatible interactions, attacked plant epidermal cells defend themselves against attempted
Torben Gjetting +3 more
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Orchestrating single-cell analysis with Bioconductor [PDF]
Nature Methods, 2019 AbstractRecent developments in experimental technologies such as single-cell RNA sequencing have enabled the profiling a high-dimensional number of genome-wide features in individual cells, inspiring the formation of large-scale data generation projects quantifying unprecedented levels of biological variation at the single-cell level.
Lindsay N. Carpp +19 more
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Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis
Molecules, 2016 This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1) prototype demonstration of single-cell encapsulation in microfluidic droplets;
Na Wen +6 more
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Advances of Single-Cell Protein Analysis [PDF]
Cells, 2020 Proteins play a significant role in the key activities of cells. Single-cell protein analysis provides crucial insights in studying cellular heterogeneities. However, the low abundance and enormous complexity of the proteome posit challenges in analyzing protein expressions at the single-cell level.
Lixing Liu +3 more
openaire +4 more sources