Results 31 to 40 of about 32,538,527 (305)

Exploring cell heterogeneity: applications of single-probe single cell mass spectrometry for subpopulation analysis [PDF]

, 2023
Mass spectrometry (MS) has become an indispensable tool for transformed metabolomics studies, whereas exploring single-cell metabolomic profiles remains a challenge due to limited techniques and suitable algorithms.
Nguyen, Tra
core  

Analysis of the paired TCR α- and β-chains of single human T cells. [PDF]

, 2012
Analysis of the paired i.e. matching TCR α- and β-chain rearrangements of single human T cells is required for a precise investigation of clonal diversity, tissue distribution and specificity of protective and pathologic T-cell mediated immune responses.
Dornmair, K., Besgen, P., Held, K., Bhonsle, L., Vollmer, Sigrid, Song-Min Kim, Jens Nickel, Anna Backes, Held, Kathrin, Vollmer, S., Joerg C Prinz, Backes Anna, Backes, A., Backes, Anna, Bhonsle, Latika, Kim Song-Min, Nickel, Jens, Sigrid Vollmer, Nickel, J., Kim, Song-Min, Klaus Dornmair, Dornmair, Klaus, Kim, S., Besgen Petra, Petra Besgen, Vollmer Sigrid, Prinz, Joerg C., Prinz Joerg C., Latika Bhonsle, Bhonsle Latika, Besgen, Petra, Held Kathrin, Prinz, J., Kathrin Held, Nickel Jens, Dornmair Klaus   +35 more
core   +2 more sources

Single-Cell Culture in Microwells

, 2012
In order to better understand cellular processes and behavior, a controlled way of studying high numbers of single cells and their clone formation is greatly needed. This chapter describes a microwell plate with 672 wells in a standard array/slide format,
Lindström, S., Helene Andersson-Svahn, Sara Lindström, Andersson-Svahn, Helene,   +3 more
core   +1 more source

Microfluidic enrichment of proteolytic microbial consortia from sewage sludge

Applied and Environmental Microbiology
Proteolytic microbial consortia are key drivers of protein hydrolysis in complex organic substrates. In anaerobic digestion systems, such as those used for biogas production from sewage sludge, this process constitutes the initial and rate-limiting step.
Luca Potenza, Valentina Smacchia, Łukasz Drewniak, Tomasz S. Kaminski   +3 more
doaj   +1 more source

Laser-fabricated cell patterning stencil for single cell analysis

BMC Biotechnology, 2017
Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications.
Jacob J. Messner, Honor L. Glenn, Deirdre R. Meldrum   +2 more
doaj   +1 more source

Single-cell states in the estrogen response of breast cancer cell lines [PDF]

, 2014
Estrogen responsive breast cancer cell lines have been extensively studied to characterize transcriptional patterns in hormone-responsive tumors. Nevertheless, due to current technological limitations, genome-wide studies have typically been limited to ...
Andrea Gamba, Nassa, Giovanni, Roberto Amendola, Mario Nicodemi (81682), Sach Mukherjee (19432), Chris J Oates, Francesco Paolo Casale (526999), Nicodemi, M, Weisz, Alessandro, Gamba, A, Jonathan, W., Alessandro Weisz (90872), Alessandro Weisz, Giorgio Giurato (527000), Giorgio Giurato, Gamba, Andrea, Cora', Davide, Chris J. Oates, Gamba, Andrea Antonio, Armond, Jonathan W., Weisz, A, Amendola, Roberto, Mukherjee, Sach, Casale, Francesco Paolo, Oates, Chris J., Jonathan W. Armond, Mario Nicodemi, Oates, CJ, Giovanni Nassa, Davide Corá, Nassa, G, Corá, Davide, Ro Weisz, Mukherjee, S, Giovanni Nassa (527001), Chris J. Oates (527003), Francesco Paolo Casale, Giurato, Giorgio, Andrea Gamba (181217), Nicodemi, Mario, Sach Mukherjee, Davide Cor?, Giurato, G, Corá, D, Chris, J., Jonathan W Armond, Davide Corá (5649682), Armond, JW, Jonathan W. Armond (527002), Casale, FP   +49 more
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Single-cell profiling of transcriptome and histone modifications with EpiDamID [PDF]

, 2022
Recent advances in single-cell sequencing technologies have enabled simultaneous measurement of multiple cellular modalities, but the combined detection of histone post-translational modifications and transcription at single-cell resolution has remained ...
Rang, Franka J, Sato, Yuko, Nguyen, Phong D, de Luca, Kim L, Rang, Franka J., de Vries, Sandra S, Bakkers, Jeroen, Nguyen, Phong D., de Vries, Sandra S., de Luca, Kim L., Kind, Jop, Boele, Ellen, Guerreiro, Isabel, Valdes-Quezada, Christian, Kimura, Hiroshi   +14 more
core   +1 more source

Artificial intelligence‐assisted automatic and index‐based microbial single‐cell sorting system for One‐Cell‐One‐Tube

mLife, 2022
Identification, sorting, and sequencing of individual cells directly from in situ samples have great potential for in‐depth analysis of the structure and function of microbiomes.
Zhidian Diao, Lingyan Kan, Yilong Zhao, Huaibo Yang, Jingyun Song, Chen Wang, Yang Liu, Fengli Zhang, Teng Xu, Rongze Chen, Yuetong Ji, Xixian Wang, Xiaoyan Jing, Jian Xu, Yuandong Li, Bo Ma   +15 more
doaj   +1 more source

Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy [PDF]

, 2014
In this study we use a novel approach to quantitatively investigate mechanical and interfacial properties of clonal β-cells using AFM-Single Cell Force Spectroscopy (SCFS). MIN6 cells were incubated for 48hrs with 0.5mM Ca2+ ± the calcimimetic R568 (1μM).
Younis, Mustafa Y.G., Hills, Claire E., Younis, Mustafa Y. G., Squires, Paul E., Liu, Kuo-Kang, Siamantouras, Eleftherios   +5 more
core   +1 more source

Single-cell electrical characterization

, 2021
This chapter provides an introduction to single-cell electrical characterization techniques and related devices, which play a key role in the framework of label-free biosensing for diagnostics, drug development, and life science.
Cottet, Jonathan, Federica Caselli, Jonathan Cottet, Caselli, Federica   +3 more
core   +1 more source