Results 101 to 110 of about 2,352,277 (290)

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

Transcriptional profiling of single fiber cells in a transgenic paradigm of an inherited childhood cataract reveals absence of molecular heterogeneity. [PDF]

, 2019
Our recent single-cell transcriptomic analysis has demonstrated that heterogeneous transcriptional activity attends molecular transition from the nascent to terminally differentiated fiber cells in the developing mouse lens.
Bhat, Suraj P, Elashoff, David, Gangalum, Rajendra K, Kashyap, Raj K, Kim, Dongjae, Mangul, Serghei, Zhou, Xinkai   +6 more
core  

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Transcriptomic HEPN1 signatures predict treatment response in low grade glioma

Discover Oncology
Background Iron-dependent ferroptosis emerges as a promising therapeutic avenue for glioma treatment, with ferroptosis-related genes such as HEPN1 offering potential as critical biomarkers when analyzed at single-cell resolution.
Lei Jiao, Shuhua Fang, Jianbo Zhang
doaj   +1 more source

From silencing to gene expression: real-time analysis in single cells.

Cell, 2004
We have developed an inducible system to visualize gene expression at the levels of DNA, RNA and protein in living cells. The system is composed of a 200 copy transgene array integrated into a euchromatic region of chromosome 1 in human U2OS cells. The condensed array is heterochromatic as it is associated with HP1, histone H3 methylated at lysine 9 ...
Janicki, Susan M, Tsukamoto, Toshiro, Salghetti, Simone E, Tansey, William P, Sachidanandam, Ravi, Prasanth, Kannanganattu V, Ried, Thomas, Shav-Tal, Yaron, Bertrand, Edouard, Singer, Robert H, Spector, David L   +10 more
openaire   +2 more sources

Conserved structural motifs in PAS, LOV, and CRY proteins regulate circadian rhythms and are therapeutic targets

FEBS Letters, EarlyView.
Cryptochrome and PAS/LOV proteins play intricate roles in circadian clocks where they act as both sensors and mediators of protein–protein interactions. Their ubiquitous presence in signaling networks has positioned them as targets for small‐molecule therapeutics. This review provides a structural introduction to these protein families.
Eric D. Brinckman, Anna E. Lester, Brian D. Zoltowski   +2 more
wiley   +1 more source

Single cell visualization of yeast gene expression shows correlation of epigenetic switching between multiple heterochromatic regions through multiple generations.

PLoS Biology, 2013
Differences in gene expression between individual cells can be mediated by epigenetic regulation; thus, methods that enable detailed analyses of single cells are crucial to understanding this phenomenon.
Yasunobu Mano, Tetsuya J Kobayashi, Jun-Ichi Nakayama, Hiroyuki Uchida, Masaya Oki   +4 more
doaj   +1 more source

Whole-transcriptome, high-throughput RNA sequence analysis of the bovine macrophage response to Mycobacterium bovis infection in vitro [PDF]

, 2013
BACKGROUND: Mycobacterium bovis, the causative agent of bovine tuberculosis, is an intracellular pathogen that can persist inside host macrophages during infection via a diverse range of mechanisms that subvert the host immune response.
Browne, JA, Conlon, KM, Gordon, SV, Gormley, E, Hokamp, K, Killick, KE, Loftus, BJ, Lohan, AJ, MacHugh, DE, Magee, DA, Nalpas, NC, Park, SD, Rue-Albrecht, K, Taraktsoglou, M   +13 more
core   +2 more sources

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

Quantitative single-cell splicing analysis reveals an ‘economy of scale’ filter for gene expression [PDF]

, 2018
In eukaryotic cells, splicing affects the fate of each pre-mRNA transcript, helping to determine whether it is ultimately processed into an mRNA, or degraded. The efficiency of splicing plays a key role in gene expression.
Ding, Fangyuan, Elowitz, Michael B.
core