Results 11 to 20 of about 642,878 (258)
Single-cell RNA sequencing in orthopedic research
Bone Research, 2023 Although previous RNA sequencing methods have been widely used in orthopedic research and have provided ideas for therapeutic strategies, the specific mechanisms of some orthopedic disorders, including osteoarthritis, lumbar disc herniation, rheumatoid ...
Tao Wang +5 more
doaj +3 more sources
Single-cell RNA-sequencing of the brain [PDF]
Clinical and Translational Medicine, 2017 Single-cell RNA-sequencing (scRNA-seq) is revolutionizing our understanding of the genomic, transcriptomic and epigenomic landscapes of cells within organs.
Raquel Cuevas-Diaz Duran +2 more
doaj +3 more sources
From bulk, single-cell to spatial RNA sequencing [PDF]
International Journal of Oral Science, 2021 RNA sequencing (RNAseq) can reveal gene fusions, splicing variants, mutations/indels in addition to differential gene expression, thus providing a more complete genetic picture than DNA sequencing.
Xinmin Li, Cun-Yu Wang
doaj +5 more sources
Single-cell RNA sequencing technologies and bioinformatics pipelines
Experimental and Molecular Medicine, 2018 Genetic data: Zooming in on single cells Showing which genes are expressed, or switched on, in individual cells may help to reveal the first signs of disease.
Byungjin Hwang, Ji Hyun Lee, Duhee Bang
doaj +3 more sources
SMARTer single cell total RNA sequencing [PDF]
Nucleic Acids Research, 2019 Abstract Single cell RNA sequencing methods have been increasingly used to understand cellular heterogeneity. Nevertheless, most of these methods suffer from one or more limitations, such as focusing only on polyadenylated RNA, sequencing of only the 3′ end of the transcript, an exuberant fraction of reads mapping to ribosomal RNA ...
Karen Verboom +12 more
openaire +5 more sources
Single-cell RNA sequencing in juvenile idiopathic arthritis
Genes and DiseasesJuvenile idiopathic arthritis (JIA) is one of the most common chronic inflammatory rheumatic diseases in children, with onset before age 16 and lasting for more than 6 weeks.
Xiwen Luo, Xuemei Tang
doaj +3 more sources
Single‐cell
Cell Proliferation, 2023 AbstractOsteoarthritis is a progressive and heterogeneous joint disease with complex pathogenesis. The various phenotypes associated with each patient suggest that better subgrouping of tissues associated with genotypes in different phases of osteoarthritis may provide new insights into the onset and progression of the disease.
Yuyuan Gu +5 more
openaire +2 more sources
Transcriptomics and single‐cell RNA‐sequencing [PDF]
Respirology, 2018 ABSTRACTThe past four decades have yielded advances in molecular biology allowing detailed characterization of the cellular genome and the transcriptome: the complete set of RNA species transcribed by a cell or tissue. Through transcriptomics and next‐generation sequencing, we can now attain an unprecedented level of detail in understanding cellular ...
Daniel C. Chambers +3 more
openaire +3 more sources
Single-cell RNA sequencing v1 [PDF]
, 2021 Single-cell RNA sequencing (scRNA-seq) allows for transcriptional profiling of individual cells within a heterogenous sample. This protocol describes a method for performing scRNA-seq using handpicked pancreatic islets from organ donors.
Klaus H. Kaestner Lab, Suzanne Shapira
openaire +1 more source
Single-cell RNA Sequencing in Immunology
Current Genomics, 2020 The complex immune system is involved in multiple pathological processes. Single-cell RNA sequencing (scRNA-seq) is able to analyze complex cell mixtures correct to a single cell and single molecule, thus is qualified to analyze immune reactions in several diseases.
Yirui Cao +3 more
openaire +3 more sources