Results 131 to 140 of about 1,166,241 (315)

Single cell RNA sequencing: modern approaches and achievements

RUDN Journal of Medicine
Relevance. Single-cell RNA sequencing (scRNA-seq) is a modern approach to studying the diversity and heterogeneity of RNA transcripts in individual cells, as well as to identifying the composition of different cell types and functions in organisms ...
Artem E. Gusev, Petr V. Chernov, Nikolai A. Dmitriev, Ilya A. Kofiadi   +3 more
doaj   +1 more source

Advances in single-cell sequencing technologies and applications in cancer

, 2018
Broadly speaking, this thesis combines two subjects: advances of single cell sequencing technolgies, and the application of single cell sequencing technologies in a cancer setting. Since the first single cell sequencing technologies were established, the
Kester, Lennart, Kester, Lennart Anne
core  

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

The role of miR‐335‐5p in the redifferentiation of BRAF p.V600E thyroid cancers

Molecular Oncology, EarlyView.
The BRAF p.V600E mutation promotes thyroid cancer dedifferentiation and radioiodine resistance. Using a network approach, we identified miR‐335‐5p as a key regulator of BRAF‐mutated thyroid tumors. Restoring miR‐335‐5p increased thyroid‐specific gene expression and iodine uptake in cells and organoids.
Valeria Pecce, Simone Bini, Marialuisa Sponziello, Giorgio Grani, Giulia Fiscon, Paola Paci, Lorenzo Farina, Rosa Falcone, Valentina Maggisano, Sebastiano Filetti, Cosimo Durante, Antonella Verrienti   +11 more
wiley   +1 more source

Single-cell sequencing [PDF]

Nature Methods, 2013
openaire   +3 more sources

Single‐Cell Sequencing Reveals Functional Alterations in Tuberculosis

Advanced Science
Despite its importance, the functional heterogeneity surrounding the dynamics of interactions between mycobacterium tuberculosis and human immune cells in determining host immune strength and tuberculosis (TB) outcomes, remains far from understood.
Mengyuan Lyu, Gaolian Xu, Jian Zhou, Julien Reboud, Yili Wang, Hongli Lai, Yi Chen, Yanbing Zhou, Guiying Zhu, Jonathan M. Cooper, Binwu Ying   +10 more
doaj   +1 more source

TRAIL‐PEG‐Apt‐PLGA nanosystem as an aptamer‐targeted drug delivery system potential for triple‐negative breast cancer therapy using in vivo mouse model

Molecular Oncology, EarlyView.
Aptamers are used both therapeutically and as targeting agents in cancer treatment. We developed an aptamer‐targeted PLGA–TRAIL nanosystem that exhibited superior therapeutic efficacy in NOD/SCID breast cancer models. This nanosystem represents a novel biotechnological drug candidate for suppressing resistance development in breast cancer.
Gulen Melike Demirbolat, Aslihan Kucuk, Omer Erdogan, Samed Ozer, Bensu Kozan, Tugba Cuceli, Erkan Gumus, Evrim Cevik, Ozge Cevik   +8 more
wiley   +1 more source

Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment

Molecular Oncology, EarlyView.
This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley   +1 more source

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Biosensors
Single-cell RNA sequencing is a high-throughput novel method that provides transcriptional profiling of individual cells within biological samples.
Roozbeh Abedini-Nassab, Fatemeh Taheri, Ali Emamgholizadeh, Hossein Naderi-Manesh   +3 more
doaj   +1 more source

Single-cell 5hmC sequencing reveals chromosome-wide cell-to-cell variability and enables lineage reconstruction

, 2016
The epigenetic DNA modification 5-hydroxymethylcytosine (5hmC) has crucial roles in development and gene regulation. Quantifying the abundance of this epigenetic mark at the single-cell level could enable us to understand its roles.
Dey, Siddharth S, Jean-Charles Boisset, Siddharth S Dey, Crosetto, Nicola, Dey, Siddharth S., Boisset, Jean Charles, Mooijman, Dylan, Nicola Crosetto, Dylan Mooijman, Alexander van Oudenaarden, Boisset, Jean-Charles, van Oudenaarden, Alexander   +11 more
core   +1 more source