Results 111 to 120 of about 1,166,241 (315)

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source

The ubiquitin‐proteasome system and autophagy as guardians of the cellular proteome

FEBS Letters, EarlyView.
This Perspective covers the three principles governing the crosstalk between the ubiquitin‐proteasome system and autophagy in cellular proteostasis: (1) a shared ubiquitin code routing substrates via shuttle factors or autophagy receptors; (2) spatial compartmentalization into phase‐separated degradation hubs and organelle‐specific modules (exemplified
Ivan Dikic
wiley   +1 more source

Single-Cell RNA Sequencing and Transcriptome Analysis Revealed the Immune Microenvironment and Gene Markers of Acute Respiratory Distress Syndrome

, 2023
Dan He,1,* Qiao Yu,2,* Xiaona Zeng,2,* Jihua Feng,2 Ruiqi Yang,2 Huan Wan,2 Ying Zhong,2 Yanli Yang,2 Ruzhi Zhao,2 Junyu Lu,3,4 Jianfeng Zhang1,2,4 1Department of General Practice, The Second Affiliated Hospital of Guangxi Medical University,
He D, Yang Y, Feng J, Zhao R, Zhang J, Yu Q, Lu J, Zeng X, Yang R, Wan H, Zhong Y   +10 more
core  

An unexpected alternative viologen electron mediator site in tungsten‐containing formate dehydrogenase

FEBS Letters, EarlyView.
An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley   +1 more source

Single-Cell Analysis Reveals a Close Relationship between Differentiating Dopamine and Subthalamic Nucleus Neuronal Lineages

, 2017
Stem cell engineering and grafting of mesencephalic dopamine (mesDA) neurons is a promising strategy for brain repair in Parkinson's disease (PD). Refinement of differentiation protocols to optimize this approach will require deeper understanding of ...
Perlmann, Thomas,, Storvall, Helena,, Gillberg, L, Perlmann, Thomas., Joodmardi, Eliza, Kirkeby, A, Gillberg, Linda,, Parmar, Malin,, Kirkeby, Agnete, Perlmann, Thomas, Lahti, Laura,, Sandberg, Rickard,, Nolbrant, Sara, Dahl, Lina,, Bjorklund, AK, Kee, N,, Storvall, H, Dahl, L, Kee, Nigel, Lahti, L, Björklund, Åsa K., Volakakis, Nikolaos, Kee, Nigel,, Dahl, Lina, Joodmardi, E, Parmar, Malin, Volakakis, N, Björklund, Åsa K.,, Bjoerklund, Aasa K., Joodmardi, Eliza,, Gillberg, Linda, Lahti, Laura, Storvall, Helena, Volakakis, Nikolaos,, Nolbrant, Sara,, Sandberg, Rickard, Nolbrant, S, Kirkeby, Agnete,   +37 more
core   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

Single-cell exome sequencing and monoclonal evolution of a JAK2-negative myeloproliferative neoplasm

, 2012
SummaryTumor heterogeneity presents a challenge for inferring clonal evolution and driver gene identification. Here, we describe a method for analyzing the cancer genome at a single-cell nucleotide level.
Zhang, Ruren, Xu, Xun, Yu, Chang, Yang, Xu, Wang, Wen, Ye, Xiaofei, Hou, Yong, Wu, Kui, Chen, Yan, Bolund, Lars, Zhang, Bo, Zheng, Hancheng, Ye, Chen, Kristiansen, Karsten, Li, Qibin, Li, Jingxiang, Yang, Huanming, Tao, Ye, Wang, Jian, Zhang, Xiuqing, Jian, Min, Chen, Lei, Liang, Jie, Song, Luting, Wu, Renhua, Wang, Jun, Zhu, Ping, Li, Yingrui, Mao, Mao, Gong, Zhuolin, Laurie, Goodman, Li, Fuqiang, Yang, Lin, Xie, Wei, Liu, Xin, Shao, Di, Wu, Hanjie, Lu, Zuhong, Yao, Xiaotian, Gu, Ning   +39 more
core   +1 more source

Molecular mechanisms related to neutrophils in sepsis using single-cell sequencing combined with Mendelian randomization analysis

Archives of Medical Science
Introduction Material and methods Results ...
Ran Li, Yiwen Wang, Simiao Chen, Mengmeng Zhuang, Yong Sun   +4 more
doaj   +1 more source

Large scale single nucleotide polymorphism discovery in unsequenced genomes using second generation high throughput sequencing technology: applied to turkey [PDF]

, 2009
Background The development of second generation sequencing methods has enabled large scale DNA variation studies at moderate cost. For the high throughput discovery of single nucleotide polymorphisms (SNPs) in species lacking a sequenced reference genome,
Kerstens Hindrik HD, Veenendaal, A., Johan T den Dunnen, Martien AM Groenen, Bert W Dibbits, Thomas FC Chin-A-Woeng, Dunnen, J.T., den, Groenen, M.A.M., Dibbits, B.W., Kerstens, H.H.D., Chin-A-Woeng, T.F.C., Dibbits Bert W, Hindrik HD Kerstens, Richard PMA Crooijmans, Veenendaal Albertine, den Dunnen Johan T, Chin-A-Woeng Thomas FC, Crooijmans, R.P.M.A., Albertine Veenendaal, Groenen Martien AM, Crooijmans Richard PMA, den Dunnen, J.T.   +21 more
core   +1 more source

Phosphoinositides and inositol phosphates as molecular glues

FEBS Letters, EarlyView.
Inositol phosphates (IPs) and phosphoinositides (PIPs) regulate diverse eukaryotic processes. Beyond recruiting signaling proteins or acting as structural cofactors, recent studies suggest they mediate protein–protein interactions as natural molecular glues.
Aleshia Seaton‐Terry, Alexander J. Cutright, Mong Na Claire Loi, Jessica Martin, Joseph W. Gilligan, Jakub N. Kubina, Michael J. Arambula, Susanna Tanada Palmu, Daniella Zhou, Raymond D. Blind   +9 more
wiley   +1 more source