Results 41 to 50 of about 502,506 (259)

Enhancing soluble expression and purification of a bispecific scFv antibody in Escherichia coli using chaperones

Research in Pharmaceutical Sciences
Background and purpose: The use of a bispecific antibody engineered as a tandem single-chain fragment variable antibody (scFv-BsAb) offers several advantages over full-length antibodies, including enhanced tissue penetration and the potential for ...
Zahra Alidousti-Shahraki, Afrooz Amini-Dehkordi, Nafiseh Esmaeil, Vajihe Akbari   +3 more
doaj   +1 more source

VHH-Based Bispecific Antibodies Targeting Cytokine Production

Frontiers in Immunology, 2017
Proinflammatory cytokines, such as TNF, IL-6, and IL-1, play pathogenic roles in multiple diseases and are attractive targets for biologic drugs. Because proinflammatory cytokines possess non-redundant protective and immunoregulatory functions, their ...
Maxim A. Nosenko, Maxim A. Nosenko, Kamar-Sulu N. Atretkhany, Kamar-Sulu N. Atretkhany, Vladislav V. Mokhonov, Vladislav V. Mokhonov, Grigory A. Efimov, Grigory A. Efimov, Andrey A. Kruglov, Andrey A. Kruglov, Andrey A. Kruglov, Sergei V. Tillib, Sergei V. Tillib, Marina S. Drutskaya, Marina S. Drutskaya, Sergei A. Nedospasov, Sergei A. Nedospasov, Sergei A. Nedospasov, Sergei A. Nedospasov   +18 more
doaj   +1 more source

Rab14 regulates the transport of human papillomavirus to the trans‐Golgi network for infectious cell entry

FEBS Letters, EarlyView.
This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley   +1 more source

Single-chain antibody gene therapy strategy based on high-throughput screening triggers sustained antiviral activity in the body

Journal of Virology
The occurrence of viral diseases poses a huge threat and impact on human public health safety and the development of the animal and fishery industry. Here, a strain of single-chain antibody fragment, scFv-1, was isolated from the phage antibody display ...
Liang Zhao, Xue-Feng Wei, Kun Xu, Zhao Zhao, Guo Chen, Hou-Peng Wang, Bin Zhu   +6 more
doaj   +1 more source

Nanobodies and Nanobody-Based Human Heavy Chain Antibodies As Antitumor Therapeutics

Frontiers in Immunology, 2017
Monoclonal antibodies have revolutionized cancer therapy. However, delivery to tumor cells in vivo is hampered by the large size (150 kDa) of conventional antibodies. The minimal target recognition module of a conventional antibody is composed of two non-
Peter Bannas, Julia Hambach, Friedrich Koch-Nolte   +2 more
doaj   +1 more source

ABL kinase‐dependent phosphorylation of SH proteins promotes their direct interaction with CRK family SH2 domains

FEBS Letters, EarlyView.
CT10 regulator of kinase (CRK) and CRK‐Like (CRKL) are signaling adaptors driving cell adhesion, motility, differentiation, and proliferation. SH2‐domain containing (SH) proteins are enriched in YXXP motifs which when phosphorylated create preferred binding sites for CRK family SH2 domains.
Phoebe M. Cousens, Brendan W. Chandler, Anna M. Schmoker, Jaye L. Weinert, Charlotte A. Kearns, Warren T. Yacawych, Amila Šemić, Alicia M. Ebert, Bryan A. Ballif   +8 more
wiley   +1 more source

Isolation and characterization of anti c-met single chain fragment variable (scFv) antibodies

Journal of Immunotoxicology, 2017
The receptor tyrosine kinase (RTK) Met is the cell surface receptor for hepatocyte growth factor (HGF) involved in invasive growth programs during embryogenesis and tumorgenesis.
Elmira Safaie Qamsari, Zahra Sharifzadeh, Salman Bagheri, Farhad Riazi-Rad, Vahid Younesi, Mohsen Abolhassani, Sepideh Safaei Ghaderi, Behzad Baradaran, Mohammad Hossein Somi, Mehdi Yousefi   +9 more
doaj   +1 more source

Single-domain antibody fragments derived from heavy-chain antibodies: a review

Veterinární Medicína, 2012
Single-domain antibody (sdAb) fragments derived from heavy-chain antibodies of camelids and cartilaginous fish represent a new generation of therapeutic agents and immunoreagents.
L. Eyer, K. Hruska
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Cross-reactivity trends when selecting scFv antibodies against snake toxins using a phage display-based cross-panning strategy

Scientific Reports, 2023
Antibodies with cross-reactive binding and broad toxin-neutralizing capabilities are advantageous for treating indications such as infectious diseases and animal envenomings.
Christoffer V. Sørensen, Line Ledsgaard, Helen H. K. Wildenauer, Camilla H. Dahl, Tasja W. Ebersole, Markus-Frederik Bohn, Anne Ljungars, Timothy P. Jenkins, Andreas H. Laustsen   +8 more
doaj   +1 more source