Results 21 to 30 of about 502,506 (259)

Single-Chain Fragment Variable Antibody Piezoimmunosensors [PDF]

Analytical Chemistry, 2005
In this paper, we describe a novel nonlabeled biosensor with high diagnostic potential for rapid and sensitive detection of antigens in complex biological samples. The biosensor comprises a piezoimmunosensor (PZ) displaying a specially constructed recombinant antibody on its surface.
Zhihong, Shen, Gabrielle A, Stryker, Ray L, Mernaugh, Lei, Yu, Heping, Yan, Xiangqun, Zeng   +5 more
openaire   +2 more sources

Recombinant Antibodies in Veterinary Medicine: An Update

Frontiers in Veterinary Science, 2018
The production of recombinant antibodies has had a tremendous impact on several research fields, most prominently in biotechnology, immunology and medicine, enabling enormous advances in each.
Lorena Bustamante-Córdova, Edgar A. Melgoza-González, Jesús Hernández   +2 more
doaj   +1 more source

Brain Delivery of Single-Domain Antibodies: A Focus on VHH and VNAR

Pharmaceutics, 2020
Passive immunotherapy, i.e., treatment with therapeutic antibodies, has been increasingly used over the last decade in several diseases such as cancers or inflammation.
Elodie Pothin, Dominique Lesuisse, Pierre Lafaye   +2 more
doaj   +1 more source

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +1 more source

SINGLE CHAIN VARIABLE FRAGMENTS OF ANTIBODIES AGAINST DIPHTHERIA TOXIN B-SUBUNIT ISOLATED FROM PHAGE DISPLAY HUMAN ANTIBODY LIBRARY [PDF]

Biotechnologia Acta, 2014
Diphtheria toxin is an exoantigen of Corynebacterium diphtheriae that inhibits protein synthesis and kills sensitive cells. The aim of this study was to obtain human recombinant single-chain variable fragment (scFv) antibodies against receptor-binding B ...
Oliinyk O. S., Kaberniuk A. A., Kolibo D. V., Komisarenko S. V.   +3 more
doaj   +1 more source

Strategies for Bispecific Single Chain Antibody in Cancer Immunotherapy [PDF]

Journal of Cancer, 2017
Genetic engineering has resulted in more than 50 recombinant bispecific antibody formats over the past two decades. Bispecific scFv antibodies represent a successful and promising immunotherapy platform that retargets cytotoxic T cells to tumor cells, with one scFv directed to tumor-associated antigens and the other to T cells.
Zhou, Shu-juan, Wei, Jia, Su, Shu, Chen, Fang-jun, Qiu, Yu-dong, Liu, Bao-rui   +5 more
openaire   +2 more sources

Heavy chain-only antibodies with a stabilized human VH in transgenic chickens for therapeutic antibody discovery

mAbs
Heavy chain-only antibodies have found many applications where conventional heavy-light heterodimeric antibodies are not favorable. Heavy chain-only antibodies with their single antigen-binding domain offer the advantage of a smaller size and higher ...
Christine N. Vuong, Kevin M. Reynolds, Gerry S. Rivera, Baisen Zeng, Zahra Karimpourkalou, Manith Norng, Yulei Zhang, Robayet Chowdhury, Darlene Pedersen, Melissa Pantoja, Ellen Collarini, Swetha Garimalla, Shelley Izquierdo, Eric G. Vajda, Brett Antonio, Devendra B. Srivastava, Marie-Cecile van de Lavoir, Yasmina Abdiche, William Harriman, Philip A. Leighton   +19 more
doaj   +1 more source

Design Principles for Bispecific IgGs, Opportunities and Pitfalls of Artificial Disulfide Bonds

Antibodies, 2018
Bispecific antibodies (bsAbs) are antibodies with two binding sites directed at different antigens, enabling therapeutic strategies not achievable with conventional monoclonal antibodies (mAbs).
Lilach Vaks, Dana Litvak-Greenfeld, Stav Dror, LeeRon Shefet-Carasso, Galia Matatov, Limor Nahary, Shiran Shapira, Rahely Hakim, Iris Alroy, Itai Benhar   +9 more
doaj   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Anti-CXCR4 Single-Chain Variable Fragment Antibodies Have Anti-Tumor Activity

Frontiers in Oncology, 2020
Monoclonal antibodies (mAbs) are large and have limitations as cancer therapeutics. Human single-chain variable fragment (scFv) is a small antibody as a good alternative.
Guang-Quan Liang, Jing Liu, Xiao-Xin Zhou, Ze-Xiong Lin, Tao Chen, Guo Chen, Henry Wei   +6 more
doaj   +1 more source