Results 11 to 20 of about 502,506 (259)

Importance of Hypervariable Region 2 for Stability and Affinity of a Shark Single-Domain Antibody Specific for Ebola Virus Nucleoprotein. [PDF]

PLoS ONE, 2016
Single-domain antibodies derived from the unique New Antigen Receptor found in sharks have numerous potential applications, ranging from diagnostic reagents to therapeutics.
George P Anderson, Daniel D Teichler, Dan Zabetakis, Lisa C Shriver-Lake, Jinny L Liu, Stephen G Lonsdale, Sarah A Goodchild, Ellen R Goldman   +7 more
doaj   +1 more source

Single-chain Fragment variable antibody targeting cholecystokinin-B receptor for pain reduction

Neurobiology of Pain, 2021
The cholecystokinin B receptor and its neuropeptide ligand are upregulated in chronic neuropathic pain models. Single-chain Fragment variable antibodies were generated as preferred non-opioid targeting therapy blocking the cholecystokinin B receptor to ...
K.N. Westlund, M.A. Montera, A.E. Goins, S.R.A. Alles, M. Afaghpour-Becklund, R. Bartel, R. Durvasula, A. Kunamneni   +7 more
doaj   +1 more source

Shelf-Life Extension of Fc-Fused Single Chain Fragment Variable Antibodies by Lyophilization

Frontiers in Cellular and Infection Microbiology, 2021
Generation of sequence defined antibodies from universal libraries by phage display has been established over the past three decades as a robust method to cope with the increasing market demand in therapy, diagnostics and research.
Kai-Thomas Schneider, Toni Kirmann, Esther Veronika Wenzel, Esther Veronika Wenzel, Jan-Hendrik Grosch, Jan-Hendrik Grosch, Saskia Polten, Doris Meier, Marlies Becker, Paul Matejtschuk, Michael Hust, Giulio Russo, Giulio Russo, Stefan Dübel   +13 more
doaj   +1 more source

Engineered Fully Human Single-Chain Monoclonal Antibodies to PIM2 Kinase

Molecules, 2021
Proviral integration site of Moloney virus-2 (PIM2) is overexpressed in multiple human cancer cells and high level is related to poor prognosis; thus, PIM2 kinase is a rational target of anti-cancer therapeutics.
Kanasap Kaewchim, Kittirat Glab-ampai, Kodchakorn Mahasongkram, Monrat Chulanetra, Watee Seesuay, Wanpen Chaicumpa, Nitat Sookrung   +6 more
doaj   +1 more source

Microbial approach of epitope tagged MFE-23 single fragment antibodies production

JKKI (Jurnal Kedokteran dan Kesehatan Indonesia), 2020
Background: Antibodies have been investigated for future clinical application in cancer management. An antibody, MFE-23 scFv is known for its ability to bind Carcinoembryonic Antigen (CEA).
Razmaeda Sarastry
doaj   +1 more source

A multi-Fc-species system for recombinant antibody production

BMC Biotechnology, 2009
Background Genomic, transcriptomic and proteomic projects often suffer from a lack of functional validation creating a strong demand for specific and versatile antibodies.
Nizak Clément, Vielemeyer Ole, El Marjou Ahmed, Moutel Sandrine, Benaroch Philippe, Dübel Stefan, Perez Franck   +6 more
doaj   +1 more source

Optimization of the crystallizability of a single-chain antibody fragment [PDF]

Acta Crystallographica Section F Structural Biology Communications, 2014
Single-chain variable antibody fragments (scFvs) are molecules with immense therapeutic and diagnostic potential. Knowledge of their three-dimensional structure is important for understanding their antigen-binding mode as well as for protein-engineering approaches such as antibody humanization.
Jana, Škerlová, Vlastimil, Král, Milan, Fábry, Juraj, Sedláček, Václav, Veverka, Pavlína, Rezáčová   +5 more
openaire   +2 more sources

Enhancement of the neutralization ability resulting from a single amino acid change in the light chain of a chimeric antibody against SARS-CoV-2

Shanghai Jiaotong Daxue xuebao. Yixue ban, 2023
Objective·To screen the human-mouse chimeric antibodies that neutralize the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and analyse the key amino acid site changes in their light chain.Methods·Antibody heavy chain gene-humanized mice ...
YIN Zi, LIAN Chaoyang, GAO Bo, TIAN Ying, HAO Qian, YEAP Lengsiew   +5 more
doaj   +1 more source

Expression of the neurofibromatosis I gene product, neurofibromin, in blood vessel endothelial cells and smooth muscle

Neurobiology of Disease, 1995
Vascular pathology is an underestimated complication of neurofibromatosis 1 (NF1). Manifestations include renovascular stenosis with associated hypertension, cerebrovascular occlusion, visceral ischaemia and aneurysms of smaller arteries.
Karen K. Norton, Jian Xu, David H. Gutmann   +2 more
doaj   +1 more source

The eIg technology to generate Ig-like bispecific antibodies

mAbs, 2022
Bispecific antibodies have emerged as therapeutic molecules with a multitude of modes of action and applications. Here, we present a novel approach to solve the light-chain problem for the generation of bispecific Ig-like antibodies using the second ...
Lennart Kühl, Nadine Aschmoneit, Roland E. Kontermann, Oliver Seifert   +3 more
doaj   +1 more source