Results 31 to 40 of about 865,522 (266)

Application of Single-Domain Antibodies (“Nanobodies”) to Laboratory Diagnosis

Annals of Laboratory Medicine, 2021
Antibodies have proven to be central in the development of diagnostic methods over decades, moving from polyclonal antibodies to the milestone development of monoclonal antibodies.
T. Pillay, S. Muyldermans
semanticscholar   +1 more source

Engineering pH-Sensitive Single-Domain Antibodies. [PDF]

Methods Mol Biol, 2022
There is increasing interest in expanding an antibody beyond high affinity and specificity. One such feature is custom regulation of the binding event, such as pH-dependent control. Here, we provide a methodology for generating single-domain antibodies (sdAbs) that bind their antigen in a pH-dependent fashion.
Laughlin TM, Horn JR.
europepmc   +3 more sources

Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies

Frontiers in Immunology, 2021
The COVID-19 pandemic, caused by the coronavirus SARS-CoV-2, is the most severe public health event of the twenty-first century. While effective vaccines against SARS-CoV-2 have been developed, there remains an urgent need for diagnostics to quickly and ...
Q. Ye, Sha Lu, K. Corbett
semanticscholar   +1 more source

Single-Domain Antibodies for Targeting, Detection, and In Vivo Imaging of Human CD4+ Cells

bioRxiv, 2021
The advancement of new immunotherapies necessitates appropriate probes to monitor the presence and distribution of distinct immune cell populations. Considering the key role of CD4+ T cells in regulating immunological processes, we generated novel single-
B. Traenkle, P. Kaiser, Stefania Pezzana, Jennifer R Richardson, Marius Gramlich, Teresa R. Wagner, Dominik Seyfried, Melissa Weldle, Stefanie Holz, Y. Parfyonova, S. Nueske, Armin M Scholz, A. Zeck, Meike Jakobi, N. Schneiderhan-Marra, M. Schaller, A. Maurer, C. Gouttefangeas, M. Kneilling, B. Pichler, D. Sonanini, U. Rothbauer   +21 more
semanticscholar   +1 more source

Single-Domain Antibodies Efficiently Neutralize SARS-CoV-2 Variants of Concern

bioRxiv, 2021
Virus-neutralizing antibodies are one of the few treatment options for COVID-19. The evolution of SARS-CoV-2 virus has led to the emergence of virus variants with reduced sensitivity to some antibody-based therapies.
I. Favorskaya, D. Shcheblyakov, I. Esmagambetov, I. Dolzhikova, I. Alekseeva, A. I. Korobkova, D. Voronina, E. Ryabova, A. A. Derkaev, A. Kovyrshina, A. Iliukhina, A. Botikov, O. Voronina, D. Egorova, O. Zubkova, N. Ryzhova, E. Aksenova, M. Kunda, D. Logunov, B. Naroditsky, A. Gintsburg   +20 more
semanticscholar   +1 more source

Single-domain antibody screening by isPLA-seq [PDF]

Life Science Alliance, 2021
Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular ...
Yueyuan Yin, Fei Yan, Ruimin Zhou, Mingchen Li, Jinyi Ma, Zhe Liu, Zhenyi Ma   +6 more
openaire   +2 more sources

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +1 more source

Single-domain antibodies for biomedical applications [PDF]

Immunopharmacology and Immunotoxicology, 2015
Single-domain antibodies are the smallest antigen-binding units of antibodies, consisting either only of one variable domain or one engineered constant domain that solely facilitates target binding. This class of antibody derivatives comprises naturally occurring variable domains derived from camelids and sharks as well as engineered human variable or ...
Krah, Simon, Schröter, Christian, Zielonka, Stefan, Empting, Martin, Valldorf, Bernhard, Kolmar, Harald   +5 more
openaire   +3 more sources

Photobodies: Light‐Activatable Single‐Domain Antibody Fragments [PDF]

Angewandte Chemie, 2019
AbstractPhotocaged antibody fragments, termed photobodies, have been developed that are impaired in their antigen‐binding capacity and can be activated by irradiation with UV light (365 nm). This rational design concept builds on the selective photocaging of a single tyrosine in a nanobody (a single‐domain antibody fragment).
Benedikt Jedlitzke, Zahide Yilmaz, Wolfgang Dörner, Henning D. Mootz   +3 more
openaire   +3 more sources

Broad Reactivity Single Domain Antibodies against Influenza Virus and Their Applications to Vaccine Potency Testing and Immunotherapy

Biomolecules, 2021
The antigenic variability of influenza presents many challenges to the development of vaccines and immunotherapeutics. However, it is apparent that there are epitopes on the virus that have evolved to remain largely constant due to their functional ...
Andrew Tung Yep, Y. Takeuchi, O. Engelhardt, S. Hufton   +3 more
semanticscholar   +1 more source