Results 21 to 30 of about 837,563 (288)

Single-domain antibody screening by isPLA-seq [PDF]

Life Science Alliance, 2021
Single-domain antibody (sdAb) holds the promising strategies for diverse research and translational applications. Here, we describe a method for the adaptation of the in situ proximity ligation assay (isPLA) followed by sequencing (isPLA-seq) to facilitate screening of a high-sensitive, high-throughput sdAb library for a given protein at subcellular ...
Yueyuan Yin, Fei Yan, Ruimin Zhou, Mingchen Li, Jinyi Ma, Zhe Liu, Zhenyi Ma   +6 more
openaire   +2 more sources

An Inside Job: Applications of Intracellular Single Domain Antibodies

Biomolecules, 2020
Sera of camelid species contain a special kind of antibody that consists only of heavy chains. The variable antigen binding domain of these heavy chain antibodies can be expressed as a separate entity, called a single domain antibody that is ...
Eline Soetens, Marlies Ballegeer, Xavier Saelens   +2 more
doaj   +1 more source

Bglbrick strategy for the construction of single domain antibody fusions

Heliyon, 2017
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications.
Ellen R. Goldman, Aeris Broussard, George P. Anderson, Jinny L. Liu   +3 more
doaj   +1 more source

Single-domain antibodies and their formatting to combat viral infections [PDF]

, 2018
Since their discovery in the 1990s, single-domain antibodies (VHHs), also known as NanobodiesA (R), have changed the landscape of affinity reagents. The outstanding solubility, stability, and specificity of VHHs, as well as their small size, ease of ...
Ballegeer, Marlies, De Vlieger, Dorien, Rossey, Iebe, Saelens, Xavier, Schepens, Bert   +4 more
core   +2 more sources

Single-domain antibodies for biomedical applications [PDF]

Immunopharmacology and Immunotoxicology, 2015
Single-domain antibodies are the smallest antigen-binding units of antibodies, consisting either only of one variable domain or one engineered constant domain that solely facilitates target binding. This class of antibody derivatives comprises naturally occurring variable domains derived from camelids and sharks as well as engineered human variable or ...
Krah, Simon, Schröter, Christian, Zielonka, Stefan, Empting, Martin, Valldorf, Bernhard, Kolmar, Harald   +5 more
openaire   +3 more sources

Potent single-domain antibodies that arrest respiratory syncytial virus fusion protein in its prefusion state [PDF]

, 2017
Human respiratory syncytial virus (RSV) is the main cause of lower respiratory tract infections in young children. The RSV fusion protein (F) is highly conserved and is the only viral membrane protein that is essential for infection.
Chen, Man, Gilman, Morgan SA, Graham, Barney S, Kabeche, Stephanie C, Kanekiyo, Masaru, Mas, Vicente, McLellan, Jason S, Melero, José A, Rossey, Iebe, Saelens, Xavier, Schepens, Bert, Sedeyn, Koen, Spitaels, Jan, Wrapp, Daniel   +13 more
core   +2 more sources

Photobodies: Light‐Activatable Single‐Domain Antibody Fragments [PDF]

Angewandte Chemie, 2019
AbstractPhotocaged antibody fragments, termed photobodies, have been developed that are impaired in their antigen‐binding capacity and can be activated by irradiation with UV light (365 nm). This rational design concept builds on the selective photocaging of a single tyrosine in a nanobody (a single‐domain antibody fragment).
Benedikt Jedlitzke, Zahide Yilmaz, Wolfgang Dörner, Henning D. Mootz   +3 more
openaire   +3 more sources

NANOBODIES: STRUCTURE, MANUFACTURING, APPLICATION (REVIEW)

Разработка и регистрация лекарственных средств, 2019
Introduction. Single-domain antibodies (nanobodies) are composed of the heavy-chain variable domain only. Compared to conventional immunoglobulins G (IgG) nanobodies have such qualities as: high bioavailability, ability to bind epitopes that are ...
A. V. Shatalova, A. S. Yakubova, V. V. Palimpsestov, I. B. Esmagambetov   +3 more
doaj   +1 more source

Brain Delivery of Single-Domain Antibodies: A Focus on VHH and VNAR

Pharmaceutics, 2020
Passive immunotherapy, i.e., treatment with therapeutic antibodies, has been increasingly used over the last decade in several diseases such as cancers or inflammation.
Elodie Pothin, Dominique Lesuisse, Pierre Lafaye   +2 more
doaj   +1 more source

Phenotypic lentivirus screens to identify functional single domain antibodies [PDF]

, 2016
Manipulation of proteins is key in assessing their in vivo function. Although genetic ablation is straightforward, reversible and specific perturbation of protein function remains a challenge.
Brewer, Rebeccah, Brusic, Vesna, Hanke, Leo, Morin, Benjamin, Ploegh, Hidde, Schmidt, Florian I., Whelan, Sean P.J.   +6 more
core   +2 more sources