Results 41 to 50 of about 7,601,900 (287)

Evaluation of disulfide bond position to enhance the thermal stability of a highly stable single domain antibody.

PLoS ONE, 2014
Single domain antibodies are the small recombinant variable domains derived from camelid heavy-chain-only antibodies. They are renowned for their stability, in large part due to their ability to refold following thermal or chemical denaturation.
Dan Zabetakis, Mark A Olson, George P Anderson, Patricia M Legler, Ellen R Goldman   +4 more
doaj   +1 more source

Cross-Reactive and Lineage-Specific Single Domain Antibodies against Influenza B Hemagglutinin

Antibodies, 2019
Influenza B virus (IBV) circulates in the human population and causes considerable disease burden worldwide, each year. Current IBV vaccines can struggle to mount an effective cross-reactive immune response, as strains become mismatched, due to constant ...
Walter Ramage, Tiziano Gaiotto, Christina Ball, Paul Risley, George W. Carnell, Nigel Temperton, Chung Y. Cheung, Othmar G. Engelhardt, Simon E. Hufton   +8 more
doaj   +1 more source

Platelet integrin α 2 I-domain specific antibodies produced via domain specific DNA vaccination combined with variable gene phage display

, 2005
Antibodies are a powerful tool for structure/function studies of platelet proteins. However, classic immunisation frequently elicits antibody responses against domains of minor functional interest. Robust strategies to generate antibodies against defined
Deckmyn, H., Ouwehand, W.H., Hamaia, Samir W., Harmer, lan J., Stafford, P., Watkins, N.A., Deckmyn, Hans, Hughes, D.L., Hamaia, S.W., Harmer, I., Farndale, R.W., Farndale, Richard W., Hughes, Darren L., Stafford, Prachi, Schoolmeester, Anne, Watkins, Nicholas A., Schoolmeester, A., Ouwehand, Willem H.   +17 more
core   +1 more source

Enhancement of toxin- and virus-neutralizing capacity of single-domain antibody fragments by N-glycosylation [PDF]

, 2009
Single-domain antibody fragments (VHHs) have several beneficial properties as compared to conventional antibody fragments. However, their small size complicates their toxin- and virus-neutralizing capacity.
Fijten, H.P.D., Smits, C.B., H. P. D. Fijten, C. B. van Solt, Harmsen, M.M., M. M. Harmsen   +5 more
core   +1 more source

Selection of intracellular single-domain antibodies targeting the HIV-1 Vpr protein by cytoplasmic yeast two-hybrid system.

PLoS ONE, 2014
The targeting of HIV-1 using antibodies is of high interest as molecular tools to better understand the biology of the virus or as a first step toward the design of new inhibitors targeting critical viral intracellular proteins.
Julie Matz, Cécile Hérate, Jérôme Bouchet, Nelson Dusetti, Odile Gayet, Daniel Baty, Serge Benichou, Patrick Chames   +7 more
doaj   +1 more source

Enhancing neutralizing activity against influenza H1N1/PR8 by engineering a single-domain VL-M2 specific into a bivalent form.

PLoS ONE, 2022
Flu disease, with high mortality and morbidity, is caused by the influenza virus. Influenza infections are most effectively prevented through vaccination, but it requires annual reformulation due to the antigenic shift or drift of hemagglutinin and ...
Phuong Thi Hoang, Quynh Xuan Thi Luong, Seungchan Cho, Yongjun Lee, Kyungho Na, Ramadhani Qurrota Ayun, Thuy Thi Bich Vo, Taehyun Kim, Sukchan Lee   +8 more
doaj   +1 more source

Construction of Novel Bispecific Single-Domain Antibodies (BiSdAbs) with Potent Antiangiogenic Activities

Pharmaceutical Fronts, 2020
The development of bispecific antibodies (BsAbs) has had a profound impact on cancer immunotherapy. Single-domain antibodies (SdAbs) could offer advantages over other antibody formats for the generation of a BsAbs, such as small size (∼12–15 kDa), with ...
Xianglei Liu, Tianyuan Sun, Qiuhan Ge, Jianwei Zhu   +3 more
doaj   +1 more source

Organoids in pediatric cancer research

FEBS Letters, EarlyView.
Organoid technology has revolutionized cancer research, yet its application in pediatric oncology remains limited. Recent advances have enabled the development of pediatric tumor organoids, offering new insights into disease biology, treatment response, and interactions with the tumor microenvironment.
Carla Ríos Arceo, Jarno Drost
wiley   +1 more source

Trispecific T-cell engagers for dual tumor-targeting of colorectal cancer

OncoImmunology, 2022
Retargeting of T lymphocytes toward cancer cells by bispecific antibodies has demonstrated its therapeutic potential, with one such antibody approved for the treatment of acute lymphoblastic leukemia (blinatumomab) and several other in clinical trials ...
Antonio Tapia-Galisteo, Íñigo Sánchez Rodríguez, Oscar Aguilar-Sopeña, Seandean Lykke Harwood, Javier Narbona, Mariola Ferreras Gutierrez, Rocío Navarro, Laura Martín-García, Cesáreo Corbacho, Marta Compte, Javier Lacadena, Francisco J. Blanco, Patrick Chames, Pedro Roda-Navarro, Luis Álvarez-Vallina, Laura Sanz   +15 more
doaj   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source