Results 21 to 30 of about 62,512 (285)
A versatile platform for single-molecule enzymology of restriction endonuclease [PDF]
Journal of Innovative Optical Health Sciences, 2019 Enzymes are the major players for many biological processes. Fundamental studies of the enzymatic activity at the single-molecule level provides important information that is otherwise inaccessible at the ensemble level.
Xin Wang +7 more
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Rapid quantification of miRNAs using dynamic FRET-FISH
Communications Biology, 2022 The high-speed single-molecule miRNA detection technique dynamic FRET-FISH shows increased detection speed and high target specificity, detecting a single miRNA in ten seconds.
Juyoung Kim +3 more
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FRET Based Biosensor: Principle Applications Recent Advances and Challenges
Diagnostics, 2023 Förster resonance energy transfer (FRET)-based biosensors are being fabricated for specific detection of biomolecules or changes in the microenvironment.
Awadhesh Kumar Verma +3 more
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Evaluation of FRET X for single-molecule protein fingerprinting
iScience, 2021 Summary: Single-molecule protein identification is an unrealized concept with potentially ground-breaking applications in biological research. We propose a method called FRET X (Förster Resonance Energy Transfer via DNA eXchange) fingerprinting, in which
Carlos Victor de Lannoy +4 more
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Build Your Own Microscope: Step-By-Step Guide for Building a Prism-Based TIRF Microscope
Methods and Protocols, 2018 Prism-based total internal reflection fluorescence (pTIRF) microscopy is one of the most widely used techniques for the single molecule analysis of a vast range of samples including biomolecules, nanostructures, and cells, to name a few.
Dalton R. Gibbs +4 more
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Dual-probe RNA FRET-FISH in Yeast
Bio-Protocol, 2018 mRNA Fluorescence In Situ Hybridization (FISH) is a technique commonly used to profile the distribution of transcripts in cells. When combined with the common single molecule technique Fluorescence Resonance Energy Transfer (FRET), FISH can also be used ...
Gable Wadsworth +2 more
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eLife, 2021 Harnessing single-molecule FRET illuminates the structural changes necessary for a protein to fine-tune the influx of calcium when reserves inside a cell run low.
Marc Fahrner, Christoph Romanin
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PLoS ONE, 2011 Förster Resonance Energy Transfer (FRET) experiments probe molecular distances via distance dependent energy transfer from an excited donor dye to an acceptor dye.
Martin Hoefling +5 more
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ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling [PDF]
Nature Methods, 2019 ABSTRACTSingle-molecule Förster resonance energy transfer (smFRET) has become a versatile and widespread method to probe nanoscale conformation and dynamics. However, current experimental protocols often resort to molecule immobilization for long observation times and rarely approach the resolution limit of FRET-based nanoscale metrology.
Hugh Wilson, Quan Wang
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FRET Efficiency Distributions of Multistate Single Molecules [PDF]
The Journal of Physical Chemistry B, 2010 A simple analytic theory is developed to describe FRET efficiency histograms constructed from a photon trajectory generated by a molecule with multiple conformational states. The histograms are approximated by a sum of Gaussians with the parameters explicitly determined by the FRET efficiencies of the states and the rates of the transitions between the
Irina V, Gopich, Attila, Szabo
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