Single-Particle Optical Imaging for Ultrasensitive Bioanalysis [PDF]
Biosensors, 2022 The quantitative detection of critical biomolecules and in particular low-abundance biomarkers in biofluids is crucial for early-stage diagnosis and management but remains a challenge largely owing to the insufficient sensitivity of existing ensemble ...
Yujie Liu +3 more
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An encryption–decryption framework to validating single-particle imaging [PDF]
Scientific Reports, 2021 We propose an encryption–decryption framework for validating diffraction intensity volumes reconstructed using single-particle imaging (SPI) with X-ray free-electron lasers (XFELs) when the ground truth volume is absent.
Zhou Shen +3 more
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Scalable 3D reconstruction for X-ray single particle imaging with online machine learning [PDF]
Nature CommunicationsX-ray free-electron lasers offer unique capabilities for measuring the structure and dynamics of biomolecules, helping us understand the basic building blocks of life.
Jay Shenoy +4 more
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Evaluation of the performance of classification algorithms for XFEL single-particle imaging data [PDF]
IUCrJ, 2019 Using X-ray free-electron lasers (XFELs), it is possible to determine three-dimensional structures of nanoscale particles using single-particle imaging methods.
Yingchen Shi +12 more
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SpeckleNN: a unified embedding for real-time speckle pattern classification in X-ray single-particle imaging with limited labeled examples [PDF]
IUCrJ, 2023 With X-ray free-electron lasers (XFELs), it is possible to determine the three-dimensional structure of noncrystalline nanoscale particles using X-ray single-particle imaging (SPI) techniques at room temperature.
Cong Wang +4 more
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Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments [PDF]
IUCrJ, 2021 In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations.
Miklós Tegze, Gábor Bortel
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Impact of gas background on XFEL single-particle imaging [PDF]
Scientific ReportsSingle-particle imaging (SPI) using X-ray free-electron Lasers (XFELs) offers the potential to determine protein structures at high spatial and temporal resolutions without the need for crystallization or vitrification.
Tong You +2 more
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Orientation determination in single-particle x-ray coherent diffraction imaging experiments [PDF]
, 2013 Single particle diffraction imaging experiments at free-electron lasers (FEL) have a great potential for structure determination of reproducible biological specimens that can not be crystallized.
O M Yefanov, I A Vartanyants
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Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers [PDF]
Journal of Synchrotron RadiationSingle-particle imaging at X-ray free-electron lasers relies on suitable sample injection of nanoscale macromolecules and particles into the gas phase at room temperature.
Safi Rafie-Zinedine +3 more
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Water layer and radiation damage effects on the orientation recovery of proteins in single-particle imaging at an X-ray free-electron laser [PDF]
Scientific Reports, 2023 The noise caused by sample heterogeneity (including sample solvent) has been identified as one of the determinant factors for a successful X-ray single-particle imaging experiment.
Juncheng E +8 more
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