Results 1 to 10 of about 173,038 (236)

Single-Particle Optical Imaging for Ultrasensitive Bioanalysis

Biosensors, 2022
The quantitative detection of critical biomolecules and in particular low-abundance biomarkers in biofluids is crucial for early-stage diagnosis and management but remains a challenge largely owing to the insufficient sensitivity of existing ensemble ...
Yujie Liu, Binxiao Li, Baohong Liu, Kun Zhang   +3 more
doaj   +5 more sources

An encryption–decryption framework to validating single-particle imaging [PDF]

Scientific Reports, 2021
We propose an encryption–decryption framework for validating diffraction intensity volumes reconstructed using single-particle imaging (SPI) with X-ray free-electron lasers (XFELs) when the ground truth volume is absent.
Zhou Shen, Colin Zhi Wei Teo, Kartik Ayyer, N. Duane Loh   +3 more
doaj   +7 more sources

Impact of gas background on XFEL single-particle imaging [PDF]

Scientific Reports
Single-particle imaging (SPI) using X-ray free-electron Lasers (XFELs) offers the potential to determine protein structures at high spatial and temporal resolutions without the need for crystallization or vitrification.
Tong You, Johan Bielecki, Filipe R. N. C. Maia   +2 more
doaj   +6 more sources

Single-particle imaging without symmetry constraints at an X-ray free-electron laser [PDF]

IUCrJ, 2018
The analysis of a single-particle imaging (SPI) experiment performed at the AMO beamline at LCLS as part of the SPI initiative is presented here. A workflow for the three-dimensional virus reconstruction of the PR772 bacteriophage from measured single ...
Max Rose, Sergey Bobkov, Kartik Ayyer, Ruslan P. Kurta, Dmitry Dzhigaev, Young Yong Kim, Andrew J. Morgan, Chun Hong Yoon, Daniel Westphal, Johan Bielecki, Jonas A. Sellberg, Garth Williams, Filipe R.N.C. Maia, Olexander M. Yefanov, Vyacheslav Ilyin, Adrian P. Mancuso, Henry N. Chapman, Brenda G. Hogue, Andrew Aquila, Anton Barty, Ivan A. Vartanyants   +20 more
doaj   +4 more sources

An advanced workflow for single-particle imaging with the limited data at an X-ray free-electron laser [PDF]

IUCrJ, 2020
An improved analysis for single-particle imaging (SPI) experiments, using the limited data, is presented here. Results are based on a study of bacteriophage PR772 performed at the Atomic, Molecular and Optical Science instrument at the Linac Coherent ...
Dameli Assalauova, Young Yong Kim, Sergey Bobkov, Ruslan Khubbutdinov, Max Rose, Roberto Alvarez, Jakob Andreasson, Eugeniu Balaur, Alice Contreras, Hasan DeMirci, Luca Gelisio, Janos Hajdu, Mark S. Hunter, Ruslan P. Kurta, Haoyuan Li, Matthew McFadden, Reza Nazari, Peter Schwander, Anton Teslyuk, Peter Walter, P. Lourdu Xavier, Chun Hong Yoon, Sahba Zaare, Viacheslav A. Ilyin, Richard A. Kirian, Brenda G. Hogue, Andrew Aquila, Ivan A. Vartanyants   +27 more
doaj   +4 more sources

SpeckleNN: a unified embedding for real-time speckle pattern classification in X-ray single-particle imaging with limited labeled examples [PDF]

IUCrJ, 2023
With X-ray free-electron lasers (XFELs), it is possible to determine the three-dimensional structure of noncrystalline nanoscale particles using X-ray single-particle imaging (SPI) techniques at room temperature.
Cong Wang, Eric Florin, Hsing-Yin Chang, Jana Thayer, Chun Hong Yoon   +4 more
doaj   +2 more sources

Comparison of EMC and CM methods for orienting diffraction images in single-particle imaging experiments [PDF]

IUCrJ, 2021
In single-particle imaging (SPI) experiments, diffraction patterns of identical particles are recorded. The particles are injected into the X-ray free-electron laser (XFEL) beam in random orientations.
Miklós Tegze, Gábor Bortel
doaj   +2 more sources

Scalable 3D reconstruction for X-ray single particle imaging with online machine learning [PDF]

Nature Communications
X-ray free-electron lasers offer unique capabilities for measuring the structure and dynamics of biomolecules, helping us understand the basic building blocks of life.
Jay Shenoy, Axel Levy, Kartik Ayyer, Frédéric Poitevin, Gordon Wetzstein   +4 more
doaj   +2 more sources

Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers [PDF]

Journal of Synchrotron Radiation
Single-particle imaging at X-ray free-electron lasers relies on suitable sample injection of nanoscale macromolecules and particles into the gas phase at room temperature.
Safi Rafie-Zinedine, Joachim Schulz, Johan Bielecki, Michael Heymann   +3 more
doaj   +2 more sources

Current Status of Single Particle Imaging with X-ray Lasers

Applied Sciences, 2018
The advent of ultrafast X-ray free-electron lasers (XFELs) opens the tantalizing possibility of the atomic-resolution imaging of reproducible objects such as viruses, nanoparticles, single molecules, clusters, and perhaps biological cells, achieving a ...
Zhibin Sun, Jiadong Fan, Haoyuan Li, Huaidong Jiang   +3 more
doaj   +3 more sources