Results 21 to 30 of about 1,316,697 (275)

Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources. [PDF]

Struct Dyn, 2020
X-ray free electron lasers (XFELs) now routinely produce millijoule level pulses of x-ray photons with tens of femtoseconds duration. Such x-ray intensities gave rise to the idea that weakly scattering particles—perhaps single biomolecules or viruses ...
Bielecki J, Maia FRNC, Mancuso AP.
europepmc   +2 more sources

Scalable 3D reconstruction for X-ray single particle imaging with online machine learning [PDF]

Nature Communications
X-ray free-electron lasers offer unique capabilities for measuring the structure and dynamics of biomolecules, helping us understand the basic building blocks of life.
Jay Shenoy, Axel Levy, Kartik Ayyer, Frédéric Poitevin, Gordon Wetzstein   +4 more
doaj   +2 more sources

Impact of gas background on XFEL single-particle imaging [PDF]

Scientific Reports
Single-particle imaging (SPI) using X-ray free-electron Lasers (XFELs) offers the potential to determine protein structures at high spatial and temporal resolutions without the need for crystallization or vitrification.
Tong You, Johan Bielecki, Filipe R. N. C. Maia   +2 more
doaj   +2 more sources

Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers [PDF]

Journal of Synchrotron Radiation
Single-particle imaging at X-ray free-electron lasers relies on suitable sample injection of nanoscale macromolecules and particles into the gas phase at room temperature.
Safi Rafie-Zinedine, Joachim Schulz, Johan Bielecki, Michael Heymann   +3 more
doaj   +2 more sources

Fluorescent sp³ Defect-Tailored Carbon Nanotubes Enable NIR-II Single Particle Imaging in Live Brain Slices at Ultra-Low Excitation Doses. [PDF]

Sci Rep, 2020
Cellular and tissue imaging in the second near-infrared window (NIR-II, ~1000–1350 nm) is advantageous for in vivo studies because of low light extinction by biological constituents at these wavelengths.
Mandal AK, Wu X, Ferreira JS, Kim M, Powell LR, Kwon H, Groc L, Wang Y, Cognet L.   +8 more
europepmc   +2 more sources

Current Status of Single Particle Imaging with X-ray Lasers

Applied Sciences, 2018
The advent of ultrafast X-ray free-electron lasers (XFELs) opens the tantalizing possibility of the atomic-resolution imaging of reproducible objects such as viruses, nanoparticles, single molecules, clusters, and perhaps biological cells, achieving a ...
Zhibin Sun, Jiadong Fan, Haoyuan Li, Huaidong Jiang   +3 more
doaj   +2 more sources

Water layer and radiation damage effects on the orientation recovery of proteins in single-particle imaging at an X-ray free-electron laser [PDF]

Scientific Reports, 2023
The noise caused by sample heterogeneity (including sample solvent) has been identified as one of the determinant factors for a successful X-ray single-particle imaging experiment.
Juncheng E, Michal Stransky, Zhou Shen, Zoltan Jurek, Carsten Fortmann-Grote, Richard Bean, Robin Santra, Beata Ziaja, Adrian P. Mancuso   +8 more
doaj   +2 more sources

Systematic characterization of extracellular vesicle sorting domains and quantification at the single molecule - single vesicle level by fluorescence correlation spectroscopy and single particle imaging. [PDF]

J Extracell Vesicles, 2019
Extracellular vesicles (EV) convey biological information by transmitting macromolecules between cells and tissues and are of great promise as pharmaceutical nanocarriers, and as therapeutic per se. Strategies for customizing the EV surface and cargo are
Corso G, Heusermann W, Trojer D, Görgens A, Steib E, Voshol J, Graff A, Genoud C, Lee Y, Hean J, Nordin JZ, Wiklander OPB, El Andaloussi S, Meisner-Kober N.   +13 more
europepmc   +2 more sources

Expansion-maximization-compression algorithm with spherical harmonics for single particle imaging with x-ray lasers. [PDF]

Physical Review E, 2016
In three-dimensional (3D) single particle imaging with x-ray free-electron lasers, particle orientation is not recorded during measurement but is instead recovered as a necessary step in the reconstruction of a 3D image from the diffraction data. Here we
Julien Flamant, N. Le Bihan, Andrew V. Martin, J. Manton   +3 more
semanticscholar   +4 more sources

Evaluation of the performance of classification algorithms for XFEL single-particle imaging data [PDF]

IUCrJ, 2019
Using X-ray free-electron lasers (XFELs), it is possible to determine three-dimensional structures of nanoscale particles using single-particle imaging methods.
Yingchen Shi, Ke Yin, Xuecheng Tai, Hasan DeMirci, Ahmad Hosseinizadeh, Brenda G. Hogue, Haoyuan Li, Abbas Ourmazd, Peter Schwander, Ivan A. Vartanyants, Chun Hong Yoon, Andrew Aquila, Haiguang Liu   +12 more
doaj   +2 more sources