Results 21 to 30 of about 6,562,855 (307)

Cooperativity-Dependent Folding of Single-Stranded DNA

Physical Review X, 2021
The folding of biological macromolecules is a fundamental process of which we lack a full comprehension. Mostly studied in proteins and RNA, single-stranded DNA (ssDNA) also folds, at physiological salt conditions, by forming nonspecific secondary ...
X. Viader-Godoy, C. R. Pulido, B. Ibarra, M. Manosas, F. Ritort   +4 more
doaj   +1 more source

Binding dynamics of a monomeric SSB protein to DNA : a single-molecule multi-process approach [PDF]

, 2015
People Programme of the European Union’s Seventh Framework Programme [REA 334496 to B.E.B.]; Leonardo da Vinci European Union Programme (to M.F.G.); Wellcome Trust [099149/Z/12/Z, 091825/Z/10/Z]. Funding for open access charge: Wellcome Trust; University
J. Carlos Penedo, Maria Figueira-gonzalez, White, Malcolm F, Ackermann, Katrin, Katrin Ackermann, Penedo, Carlos, Peregrina, Jose Ramon, Bode, Bela Ernest, Michael J. Morten, Figueira-Gonzalez, Maria, Malcolm F. White, Bela E. Bode, Morten, Michael John, Jose R. Peregrina   +13 more
core   +1 more source

Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications [PDF]

, 2004
Ros R, Eckel R, Bartels FW, et al. Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications. Journal of Biotechnology.
Baumgarth, Birgit, Wilking, Sven David, Ros, Robert, Bartels, Frank Wilco, Sischka, Andy, Eckel, Rainer, Pühler, Alfred ; https://orcid.org/, Sewald, Norbert ; https://orcid.org/, Becker, Anke, Anselmetti, Dario ; https://orcid.org/   +9 more
core   +1 more source

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia, Webb, Martin R, Mashanov, Gregory I, Chisty, Liisa T, Toseland, Christopher P., Dillingham, Mark S, Toseland, Christopher P, Molloy, Justin E   +7 more
core   +1 more source

Expression, purification, and solution-state NMR analysis of the two human single-stranded DNA-binding proteins hSSB1 (NABP2/OBFC2B) and hSSB2 (NAPB1/OBFC2A)

, 2021
Single-stranded DNA-binding proteins (SSBs) are essential to all living organisms as protectors and guardians of the genome. Apart from the well-characterized RPA, humans have also evolved two further SSBs, termed hSSB1 and hSSB2. Over the last few years,
Cubeddu, Liza (R16726), Gamsjaeger, Roland (R16936), Teegan Lawson, El-Kamand, Serene (S33745), Lawson, Teegan (S34513), Roland Gamsjaeger, Du Plessis, Mar-Dean (S34302), Mar-Dean Du Plessis, Liza Cubeddu, Serene El-Kamand   +9 more
core   +1 more source

Continuous Approximation for Interaction Energy Transfer of DNA through Lipid Bilayers

Discrete Dynamics in Nature and Society, 2018
In this study the interaction energies for single-stranded DNA and double-stranded DNA molecules with a lipid bilayer are investigated. The 6-12 Lennard-Jones potential and continuous approximation are used to derive analytical expressions for these ...
Mansoor H. Alshehri
doaj   +1 more source

The Characteristics of New SSB Proteins from Metagenomic Libraries and Their Use in Biotech Applications

Proceedings, 2020
Single-stranded DNA binding proteins (SSBs) bind to single-stranded DNA in a sequence-independent manner to prevent formation of secondary structures and protect DNA from nuclease degradation.
Olesia Werbowy, Aleksandra Stefańska-Kaźmierczak, Agata Jurczak-Kurek, Karolina Kwiatkowska-Semrau, Anna-Karina Kaczorowska, Magdalena Płotka, Olafur H. Fridjonsson, Gudmundur O. Hreggvidsson, Slawomir Dąbrowski, Tadeusz Kaczorowski   +9 more
doaj   +1 more source

Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Nanomaterials, 2013
Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process.
Po-Jung Jimmy Huang, Juewen Liu
doaj   +1 more source

Variable termination sites of DNA polymerases encountering a DNA-protein cross-link. [PDF]

PLoS ONE, 2018
DNA-protein cross-links (DPCs) are important DNA lesions induced by endogenous crosslinking agents such as formaldehyde or acetaldehyde, as well as ionizing radiation, cancer chemotherapeutic drugs, and abortive action of some enzymes.
Anna V Yudkina, Antonina P Dvornikova, Dmitry O Zharkov   +2 more
doaj   +1 more source

Molecular mechanisms and kinetics between DNA and DNA binding ligands [PDF]

, 2005
Sischka A, Tönsing K, Eckel R, et al. Molecular mechanisms and kinetics between DNA and DNA binding ligands. Biophysical Journal. 2005;88(1):404-411.Mechanical properties of single double-stranded DNA (dsDNA) in the presence of different binding ligands ...
Anselmetti, Dario, Wilking, Sven David, Ros, Robert, Toensing, Katja, Sischka, Andy, Eckel, Rainer, Sewald, Norbert, Tönsing, Katja, Sewald, Norbert ; https://orcid.org/, Anselmetti, Dario ; https://orcid.org/   +9 more
core   +1 more source