Results 21 to 30 of about 367,917 (307)

The Characteristics of New SSB Proteins from Metagenomic Libraries and Their Use in Biotech Applications

Proceedings, 2020
Single-stranded DNA binding proteins (SSBs) bind to single-stranded DNA in a sequence-independent manner to prevent formation of secondary structures and protect DNA from nuclease degradation.
Olesia Werbowy, Aleksandra Stefańska-Kaźmierczak, Agata Jurczak-Kurek, Karolina Kwiatkowska-Semrau, Anna-Karina Kaczorowska, Magdalena Płotka, Olafur H. Fridjonsson, Gudmundur O. Hreggvidsson, Slawomir Dąbrowski, Tadeusz Kaczorowski   +9 more
doaj   +1 more source

Single-stranded DNA library preparation from highly degraded DNA using T4 DNA ligase

Nucleic Acids Research, 2017
DNA library preparation for high-throughput sequencing of genomic DNA usually involves ligation of adapters to double-stranded DNA fragments. However, for highly degraded DNA, especially ancient DNA, library preparation has been found to be more ...
Marie-Theres Gansauge, Tobias Gerber, Isabelle Glocke, P. Korlević, Laurin Lippik, S. Nagel, L. Riehl, Anna Schmidt, M. Meyer   +8 more
semanticscholar   +1 more source

Variable termination sites of DNA polymerases encountering a DNA-protein cross-link. [PDF]

PLoS ONE, 2018
DNA-protein cross-links (DPCs) are important DNA lesions induced by endogenous crosslinking agents such as formaldehyde or acetaldehyde, as well as ionizing radiation, cancer chemotherapeutic drugs, and abortive action of some enzymes.
Anna V Yudkina, Antonina P Dvornikova, Dmitry O Zharkov   +2 more
doaj   +1 more source

Separation of Short Single- and Double-Stranded DNA Based on Their Adsorption Kinetics Difference on Graphene Oxide

Nanomaterials, 2013
Separation of short single- and double-stranded DNA typically requires gel electrophoresis followed by DNA extraction, which is a time consuming process.
Po-Jung Jimmy Huang, Juewen Liu
doaj   +1 more source

Genetic and biochemical identification of a novel single-stranded DNA binding complex in Haloferax volcanii

Frontiers in Microbiology, 2012
Single-stranded DNA binding proteins play an essential role in DNA replication and repair. They use oligosaccharide-binding folds, a five-stranded ß-sheet coiled into a closed barrel, to bind to single-stranded DNA thereby protecting and stabilizing
Amy eStroud, Susan eLiddell, Thorsten eAllers   +2 more
doaj   +1 more source

Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex

Nature Communications, 2022
Here the authors probe the cleavage and gate opening of single-stranded DNA by the human topoisomerase TRR using a unique single-molecule strategy to reveal structural plasticity in response to both double-stranded DNA and the helicase BLM.
Julia A. M. Bakx, Andreas S. Biebricher, Graeme A. King, Panagiotis Christodoulis, Kata Sarlós, Anna H. Bizard, Ian D. Hickson, Gijs J. L. Wuite, Erwin J. G. Peterman   +8 more
doaj   +1 more source

Programmable autonomous synthesis of single-stranded DNA

Nature Chemistry, 2017
DNA performs diverse functional roles in biology, nanotechnology, and biotechnology, but current methods for autonomously synthesizing arbitrary single-stranded DNA are limited.
Jocelyn Y. Kishi, Thomas E. Schaus, N. Gopalkrishnan, F. Xuan, Peng Yin   +4 more
semanticscholar   +1 more source

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

BioTechniques, 2015
An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples.
Nathan Wales, Christian Carøe, Marcela Sandoval-Velasco, Cristina Gamba, Ross Barnett, José Alfredo Samaniego, Jazmín Ramos Madrigal, Ludovic Orlando, M. Thomas P. Gilbert   +8 more
doaj   +1 more source

Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro. [PDF]

PLoS ONE, 2018
Prokaryotic argonautes are a unique class of nucleic acid-guided endonucleases putatively involved in cellular defense against foreign genetic elements.
Eric A Hunt, Thomas C Evans, Nathan A Tanner   +2 more
doaj   +1 more source

Robust Genome Editing with Short Single-Stranded and Long, Partially Single-Stranded DNA Donors in Caenorhabditis elegans

Genetics, 2018
A robust genome editing pipeline is critical to the vitality of a modern genetic laboratory. Previous studies have shown that Cas9 ribonucleoprotein (RNP)-based editing can be highly effective in Caenorhabditis elegans, particularly...
G. A. Dokshin, Krishna S. Ghanta, Katherine M. Piscopo, C. Mello   +3 more
semanticscholar   +1 more source