Results 21 to 30 of about 367,400 (264)

Genetic and biochemical identification of a novel single-stranded DNA binding complex in Haloferax volcanii

open access: yesFrontiers in Microbiology, 2012
Single-stranded DNA binding proteins play an essential role in DNA replication and repair. They use oligosaccharide-binding folds, a five-stranded ß-sheet coiled into a closed barrel, to bind to single-stranded DNA thereby protecting and stabilizing
Amy eStroud   +2 more
doaj   +1 more source

Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex

open access: yesNature Communications, 2022
Here the authors probe the cleavage and gate opening of single-stranded DNA by the human topoisomerase TRR using a unique single-molecule strategy to reveal structural plasticity in response to both double-stranded DNA and the helicase BLM.
Julia A. M. Bakx   +8 more
doaj   +1 more source

New insights on single-stranded versus double-stranded DNA library preparation for ancient DNA

open access: yesBioTechniques, 2015
An innovative single-stranded DNA (ssDNA) library preparation method has sparked great interest among ancient DNA (aDNA) researchers, especially after reports of endogenous DNA content increases >20-fold in some samples.
Nathan Wales   +8 more
doaj   +1 more source

Single-stranded binding proteins and helicase enhance the activity of prokaryotic argonautes in vitro. [PDF]

open access: yesPLoS ONE, 2018
Prokaryotic argonautes are a unique class of nucleic acid-guided endonucleases putatively involved in cellular defense against foreign genetic elements.
Eric A Hunt   +2 more
doaj   +1 more source

Hybrids of Nucleic Acids and Carbon Nanotubes for Nanobiotechnology

open access: yesNanomaterials, 2015
Recent progress in the combination of nucleic acids and carbon nanotubes (CNTs) has been briefly reviewed here. Since discovering the hybridization phenomenon of DNA molecules and CNTs in 2003, a large amount of fundamental and applied research has been ...
Kazuo Umemura
doaj   +1 more source

Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost. [PDF]

open access: yesPLoS ONE, 2016
Seamless cloning methods, such as co-transformation cloning, sequence- and ligation-independent cloning (SLIC) or the Gibson assembly, are essential tools for the precise construction of plasmids.
Roger M Benoit   +5 more
doaj   +1 more source

Coordinated Binding of Single-Stranded and Double-Stranded DNA by UvsX Recombinase. [PDF]

open access: yesPLoS ONE, 2013
Homologous recombination is important for the error-free repair of DNA double-strand breaks and for replication fork restart. Recombinases of the RecA/Rad51 family perform the central catalytic role in this process.
Robyn L Maher, Scott W Morrical
doaj   +1 more source

A ligation-based single-stranded library preparation method to analyze cell-free DNA and synthetic oligos

open access: yesBMC Genomics, 2019
Background Cell-free DNA (cfDNA), present in circulating blood plasma, contains information about prenatal health, organ transplant reception, and cancer presence and progression.
Christopher J. Troll   +8 more
doaj   +1 more source

Towards quantitative viromics for both double-stranded and single-stranded DNA viruses [PDF]

open access: yesPeerJ, 2016
Background Viruses strongly influence microbial population dynamics and ecosystem functions. However, our ability to quantitatively evaluate those viral impacts is limited to the few cultivated viruses and double-stranded DNA (dsDNA) viral genomes ...
Simon Roux   +8 more
doaj   +2 more sources

Folding of single‐stranded DNA on the histone octamer [PDF]

open access: yesFEBS Letters, 1985
A complex between the single‐stranded DNA of the bacteriophage M13 and the histone octamer was analyzed by electron microscopy, low‐angle X‐ray diffraction and nuclease analysis. The morphology and the diffraction pattern of the complex strongly resemble those of the nucleosome.
Caffarelli E, Leoni L, Savino M
openaire   +3 more sources

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