Results 271 to 280 of about 852,815 (296)

Increased Stability and DNA Site Discrimination of “Single Chain” Variants of the Dimeric β-Barrel DNA Binding Domain of the Human Papillomavirus E2 Transcriptional Regulator

Biochemistry, 2007
Human papillomavirus infects millions of people worldwide and is a causal agent of cervical cancer in women. The HPV E2 protein controls the expression of all viral genes through binding of its dimeric C-terminal domain (E2C) to its target DNA site. We engineered monomeric versions of the HPV16 E2C, in order to probe the link of the dimeric beta-barrel
Mariano, Dellarole, Ignacio E, Sánchez, Eleonora, Freire, Gonzalo, de Prat-Gay   +3 more
openaire   +2 more sources

Targeting retrovirus to cancer cells expressing a mutant EGF receptor by insertion of a single chain antibody variable domain in the envelope glycoprotein receptor binding lobe

Journal of Immunological Methods, 2000
We have investigated targeting of retroviral vectors to a mutant EGF receptor (EGFRvIII) that is expressed in cancers of the brain, breast, lung and ovary, but is not found in any normal tissues. An expression plasmid was made in which a single chain Fv antibody specific for EGFRvIII was inserted at a novel position within a disulphide-bonded surface ...
I A, Lorimer, S J, Lavictoire
openaire   +2 more sources

Nanobodies, single-domain antigen-binding fragments of camelid heavy-chain antibodies.

2010
Antibodies or immunoglobulins are glycoproteins produced by B-cells and play a central role in host immune defense. Antibodies can be elicited virtually against any substance. Moreover, the immune response to any given antigen can be diverse, comprising different antibodies exhibiting different affinities and/or epitope specificities.
Hassanzadeh Ghassabeh, Gholamreza, Muyldermans, Serge, Saerens, Dirk   +2 more
openaire   +1 more source

Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface

Biomolecular NMR Assignments, 2013
Single stranded DNA binding proteins (SSBs) are present in all known cellular organisms and are critical for DNA replication, recombination and repair. The SSB from the hyperthermophilic crenarchaeote Sulfolobus solfataricus (SsoSSB) has an unusual domain structure with a single DNA-binding oligonucleotide binding (OB) fold coupled to a flexible C ...
Gamsjaeger, Roland (R16936), Kariawasam, Ruvini (S31293), Touma, Christine (R17538), Kwan, Ann H., White, Malcolm F., Cubeddu, Liza (R16726)   +5 more
openaire   +2 more sources

Development of a novel and broadly applicable sandwich ELISA assay based on rabbit single-chain variable fragments and a modified Ig-binding domain of protein L fused to a polystyrene-binding peptide

Journal of Immunological Methods
Most of currently available sandwich-type enzyme-linked immunosorbent assays (ELISA) require the use of full-length animal-derived antibodies which poses welfare criticisms and are often expensive to produce. There is therefore a strong demand for the development of more affordable and animal-free methods to produce antibodies for sandwich ELISA assay.
Yamamoto, Yodai, Taniguchi, Haruka, Nguyen, Ngoc Minh, Yokoyama, Fuki, Choowongkomon, Kiattawee, Angelini, Alessandro, Horiuchi, Jun-Ichi, Kumada, Yoichi   +7 more
openaire   +3 more sources

A spacer region between the single chain antibody- and the CD3 zeta-chain domain of chimeric T cell receptor components is required for efficient ligand binding and signaling activity.

Gene therapy, 1996
The elimination of tumor cells by cytotoxic T lymphocytes (CTLs) could become the basis of a biological cancer therapy. The recognition specificity of cytotoxic T lymphocytes (CTLs) can be genetically modified by stable introduction of chimeric T cell receptor genes and thus be directed towards tumor cells.
D, Moritz, B, Groner
openaire   +1 more source

Backbone and side-chain ¹H, ¹³C and ¹⁵N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface.

Biomolecular NMR assignments, 2015
Single stranded DNA binding proteins (SSBs) are present in all known cellular organisms and are critical for DNA replication, recombination and repair. The SSB from the hyperthermophilic crenarchaeote Sulfolobus solfataricus (SsoSSB) has an unusual domain structure with a single DNA-binding oligonucleotide binding (OB) fold coupled to a flexible C ...
Roland, Gamsjaeger, Ruvini, Kariawasam, Christine, Touma, Ann H, Kwan, Malcolm F, White, Liza, Cubeddu   +5 more
openaire   +1 more source

Elicitation of broadly protective sarbecovirus immunity by receptor-binding domain nanoparticle vaccines

Cell, 2021
Alexandra C Walls, Marcos Miranda, Minh Pham   +2 more
exaly  

Advantages of Single-Domain Antigen-Binding Fragments Derived from Functional Camel Heavy-Chain Antibodied

2001
Muyldermans Serge, Conrath Katja, Vu Khoa Bang, Serrao Teresa, Busch Magnus, Backmann Natasha, Silence Karen, Lauwereys Marc, Desmyter Aline   +8 more
openaire   +1 more source

Broad neutralization of SARS-CoV-2 variants by an inhalable bispecific single-domain antibody

Cell, 2022
Cheng Li, Yan-Ning Qian, Qiyu Mao
exaly