Protocol for High-Throughput Analysis of Sister-Chromatids Contacts
STAR Protocols, 2020 Summary: Sister chromatid interactions are a key step to ensure the successful segregation of sister chromatids after replication. Our knowledge about this phenomenon is mostly based on microscopy approaches, which have some constraints such as ...
Elena Espinosa +2 more
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Folding of cohesin’s coiled coil is important for Scc2/4-induced association with chromosomes
eLife, 2021 Cohesin’s association with and translocation along chromosomal DNAs depend on an ATP hydrolysis cycle driving the association and subsequent release of DNA. This involves DNA being ‘clamped’ by Scc2 and ATP-dependent engagement of cohesin’s Smc1 and Smc3
Naomi J Petela +13 more
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A Computational Approach to Estimating Nondisjunction Frequency in Saccharomyces cerevisiae. [PDF]
, 2016 Errors segregating homologous chromosomes during meiosis result in aneuploid gametes and are the largest contributing factor to birth defects and spontaneous abortions in humans.
Burgess, Sean M, Chu, Daniel B
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Chromosome segregation in plant meiosis [PDF]
, 2014 Faithful chromosome segregation in meiosis is essential for ploidy stability over sexual life cycles. In plants, defective chromosome segregation caused by gene mutations or other factors leads to the formation of unbalanced or unreduced gametes creating
De Storme, Nico +4 more
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Structure of the Pds5-Scc1 Complex and Implications for Cohesin Function
Cell Reports, 2016 Sister chromatid cohesion is a fundamental prerequisite to faithful genome segregation. Cohesion is precisely regulated by accessory factors that modulate the stability with which the cohesin complex embraces chromosomes.
Kyle W. Muir +5 more
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The chromosomal association of the Smc5/6 complex depends on cohesion and predicts the level of sister chromatid entanglement. [PDF]
PLoS Genetics, 2014 The cohesin complex, which is essential for sister chromatid cohesion and chromosome segregation, also inhibits resolution of sister chromatid intertwinings (SCIs) by the topoisomerase Top2. The cohesin-related Smc5/6 complex (Smc5/6) instead accumulates
Kristian Jeppsson +11 more
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The Irr1/Scc3 protein implicated in chromosome segregation in Saccharomyces cerevisiae has a dual nuclear-cytoplasmic localization [PDF]
, 2017 Background Correct chromosome segregation depends on the sister chromatid cohesion complex. The essential, evolutionarily conserved regulatory protein Irr1/Scc3, is responsible for the complex loading onto DNA and for its removal.
Fronk, Jan +2 more
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Sister Chromatid Cohesion and Aneuploidy [PDF]
, 2012 In all living organisms, cells arise from the division of a pre-existing cell and inherit one copy of the genome identical to that of the mother cell. Any defect in chromosome segregation can lead to aneuploidy via chromosome gain or loss, and consequently to genetic instability, thereby paving the way for malignant growth and cancer.
Watrin, Erwan, Prigent, Claude
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Nature Communications, 2018 Sustained spindle tension applied to sister centromeres during mitosis leads to loss of sister chromatid cohesion which is known as cohesion fatigue.
Grégory Eot-Houllier +5 more
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Chl1 DNA helicase and Scc2 function in chromosome condensation through cohesin deposition. [PDF]
PLoS ONE, 2017 Chl1 DNA helicase promotes sister chromatid cohesion and associates with both the cohesion establishment acetyltransferase Eco1/Ctf7 and the DNA polymerase processivity factor PCNA that supports Eco1/Ctf7 function.
Donglai Shen, Robert V Skibbens
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