Results 91 to 100 of about 6,254 (216)
Single-molecule analysis reveals the kinetics and physiological relevance of MutL-ssDNA binding. [PDF]
PLoS ONE, 2010 DNA binding by MutL homologs (MLH/PMS) during mismatch repair (MMR) has been considered based on biochemical and genetic studies. Bulk studies with MutL and its yeast homologs Mlh1-Pms1 have suggested an integral role for a single-stranded DNA (ssDNA ...
Jonghyun Park +5 more
doaj +1 more source
RNA Polymerase Pausing during Initial Transcription [PDF]
, 2016 In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts that are either released or extended to allow RNAP to escape from the promoter.
Bauer, D.L.V. +11 more
core +1 more source
Accurate Generation of Conformational Ensembles for Intrinsically Disordered Proteins with IDPFold
Advanced Science, Volume 12, Issue 48, December 29, 2025.In brief: IDPFold predicts the conformational ensembles of intrinsically disordered proteins (IDPs) from their amino acid sequences. The atomic structures generated by IDPFold offer crucial insights into the intricate relationship between IDP structures and their diverse biological functions.2 Abstract Intrinsically disordered proteins (IDPs) play ...
Junjie Zhu +10 more
wiley +1 more source
Single-Molecule FRET Imaging of Virus Spike–Host Interactions
Viruses, 2021 As a major surface glycoprotein of enveloped viruses, the virus spike protein is a primary target for vaccines and anti-viral treatments. Current vaccines aiming at controlling the COVID-19 pandemic are mostly directed against the SARS-CoV-2 spike ...
Maolin Lu
doaj +1 more source
The smfBox is an open-source platform for single-molecule FRET
Nature Communications, 2020 Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies ...
Benjamin Ambrose +10 more
doaj +1 more source
Nature Communications, 2021 N-Methyl-D-aspartate receptors (NMDARs) activation involves closure of the GluN1 and GluN2 subunit ligand binding domains, which is regulated allosterically by the amino-terminal domain (ATD).
Vojtech Vyklicky +3 more
doaj +1 more source
Homologous Recombination under the Single-Molecule Fluorescence Microscope [PDF]
, 2019 Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein ...
Dhakal, Soma, Gibbs, Dalton R.
core +2 more sources
Metal ion induced heterogeneity in RNA folding studied by smFRET [PDF]
Coordination Chemistry Reviews, 2016 More than two decades of investigating nucleic acids and ribonucleic acids (RNA) using single molecule Forster resonance energy transfer (smFRET) have passed. It turned out that sample heterogeneity in structure and function of RNA molecules as well as folding intermediates, kinetic subpopulations, and interconversion rates of conformational states of ...
Börner, Richard +4 more
openaire +2 more sources
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
Nature Communications, 2020 Here, using cryo-EM and smFRET, Henderson et al. show how tryptophan 571 in the HIV-1 Env acts as a conformational switch during receptor-mediated viral entry and design HIV-1 Env proteins that cannot undergo conformational changes.
Rory Henderson +17 more
doaj +1 more source
Making tau amyloid models in vitro: a crucial and underestimated challenge
FEBS Letters, Volume 599, Issue 19, Page 2718-2749, October 2025.This review highlights the challenges of producing in vitro amyloid assemblies of the tau protein. We review how accurately the existing protocols mimic tau deposits found in the brain of patients affected with tauopathies. We discuss the important properties that should be considered when forming amyloids and the benchmarks that should be used to ...
Julien Broc, Clara Piersson, Yann Fichou
wiley +1 more source