Results 61 to 70 of about 6,254 (216)
Nature Communications, 2023 The VTC complex generate eukaryotic polyphosphate (polyP). Authors here reveal how the signal-activated VTC synthesize cytosolic polyP and transport polyP across vacuolar membrane into the lumen, by using cryo-EM, in-cell NMR, and smFRET analyses.
Zeyuan Guan +20 more
doaj +1 more source
Time-resolved burst variance analysis
Biophysical Reports, 2023 Quantifying biomolecular dynamics has become a major task of single-molecule fluorescence spectroscopy methods. In single-molecule Förster resonance energy transfer (smFRET), kinetic information is extracted from the stream of photons emitted by attached
Ivan Terterov +3 more
doaj +1 more source
Fluorescence lifetime analysis of smFRET with contribution of PIFE on donor and acceptor
bioRxiv, 2023 Single-molecule fluorescence resonance energy transfer (FRET) is a powerful technique based on dipole-dipole interaction between donor and acceptor fluorophores to observe inter- and intra-molecular dynamics in realtime with sensitivity to macro ...
Sina Jazani, T. Ha
semanticscholar +1 more source
Protein conformations in Tau condensates probed by smFRET and FLIM-FRET
Biophysical Journal, 2022 M. D. Quan +3 more
semanticscholar +2 more sources
Effects of DNA looping behavior using smFRET
, 2023 This study developed a single-molecule-based assay to track the looping (circularization) of individual double-stranded DNA (dsDNA) molecules in real time. DNA encodes our genetic information through a unique combination of four nucleotides: Adenine (A), Thymine (T), Cytosine (C), and Guanine (G).
West, Natalie, Cannon, Brian
openaire +1 more source
Observation of long-range tertiary interactions during ligand binding by the TPP riboswitch aptamer
eLife, 2015 The thiamine pyrophosphate (TPP) riboswitch is a cis-regulatory element in mRNA that modifies gene expression in response to TPP concentration. Its specificity is dependent upon conformational changes that take place within its aptamer domain.
Van K Duesterberg +4 more
doaj +1 more source
Nature Communications, 2023 Growing RNAs fold differently as they are transcribed, which modulates their finally adopted structures. Riboswitches regulate gene expression by structural change, which are sensitive to co-transcriptionally structural biology.
Yanyan Xue +5 more
doaj +1 more source
Crowding-induced hybridization of single DNA hairpins [PDF]
, 2015 It is clear that a crowded environment influences the structure, dynamics, and interactions of biological molecules, but the complexity of this phenomenon demands the development of new experimental and theoretical approaches.
Baltierra-Jasso, Laura E. +4 more
core +2 more sources
Nature Communications, 2023 Single-molecule experiments have changed the way we explore the physical world, yet data analysis remains time-consuming and prone to human bias. Here, we introduce Deep-LASI (Deep-Learning Assisted Single-molecule Imaging analysis), a software suite ...
Simon Wanninger +6 more
doaj +1 more source
Biomolecules, 2022 For single-molecule studies in solution, very small concentrations of dye-labelled molecules are employed in order to achieve single-molecule sensitivity.
Olessya Yukhnovets +4 more
doaj +1 more source