Results 81 to 90 of about 6,254 (216)
Efficient use of single molecule time traces to resolve kinetic rates, models and uncertainties
, 2017 Single molecule time traces reveal the time evolution of unsynchronized kinetic systems. Especially single molecule F\"orster resonance energy transfer (smFRET) provides access to enzymatically important timescales, combined with molecular distance ...
Hugel, Thorsten, Schmid, Sonja
core +1 more source
Protein Science, 2022 Ligand binding to proteins often is accompanied by conformational transitions. Here, we describe a competition assay based on single molecule Förster resonance energy transfer (smFRET) to investigate the ligand‐induced conformational changes of the ...
Hannah Maus +4 more
semanticscholar +1 more source
Differences in Src phosphorylation of PSD‐93 and PSD‐95 drive differences in scaffolding activity
Protein Science, Volume 35, Issue 2, February 2026.Abstract Scaffold proteins contain multiple binding modules that allow for co‐localization of proteins that lack a direct interaction. Evolution resulted in different combinations of binding modules that rewired existing signal transduction pathways.
Frank A. Mindlin +3 more
wiley +1 more source
AIMS Biophysics, 2018 There is increasing interest in the use of DNA polymerases (DNA pols) in next-generation sequencing strategies. These methodologies typically rely on members of the A and B family of DNA polymerases that are classified as high-fidelity DNA polymerases ...
Thomas V. Christian +1 more
doaj +1 more source
Activated GTPase movement on an RNA scaffold drives co-translational protein targeting [PDF]
, 2012 Approximately one-third of the proteome is initially destined for the eukaryotic endoplasmic reticulum or the bacterial plasma membrane. The proper localization of these proteins is mediated by a universally conserved protein-targeting machinery, the ...
Akopian, David +4 more
core +1 more source
Protein Science, Volume 35, Issue 1, January 2026.Abstract Intrinsically disordered proteins (IDPs) lack stable tertiary structure and instead exist as dynamic ensembles of conformations, playing essential roles in cellular regulation, signaling, and disease. As structural ensembles of IDPs become increasingly available through databases such as the Protein Ensemble Database (PED) and various ...
Hamidreza Ghafouri +3 more
wiley +1 more source
Calibrated Langevin dynamics simulations of intrinsically disordered proteins
, 2014 We perform extensive coarse-grained (CG) Langevin dynamics simulations of intrinsically disordered proteins (IDPs), which possess fluctuating conformational statistics between that for excluded volume random walks and collapsed globules.
Ho, Po-Yi +2 more
core +1 more source
Experiment-friendly kinetic analysis of single molecule data in and out of equilibrium
, 2016 We present a simple and robust technique to extract kinetic rate models and thermodynamic quantities from single molecule time traces. SMACKS (Single Molecule Analysis of Complex Kinetic Sequences) is a maximum likelihood approach that works equally well
Götz, Markus +2 more
core +1 more source
smFRET reveals DHX36 repetitive binding,not unfolding,of G-quadruplexes
, 2021 Our data challenge Chen et al.'s interpretation of smFRET results, i.e. the repetitive unfolding of G4 with one-base translocations. We believe that the observed oscillatory curve represents the alternate binding of DHX36 to the 3' and 5'G-tetrad of G4s, rather than the repetitive unfolding between the canonical and the transformed non-canonical G4s ...
Guo, Hai-Lei, Liu, Na-Nv, Xi, Xu-Guang
openaire +2 more sources
smFRET Probing Reveals Substrate-Dependent Conformational Dynamics of E. coli Multidrug MdfA [PDF]
Biophysical Journal, 2019 Bacterial multidrug-resistance transporters of the major facilitator superfamily are distinguished by their extraordinary ability to bind structurally diverse substrates, thus serving as a highly efficient tool to protect cells from multiple toxic substances present in their environment, including antibiotic drugs.
Yongping, Zhu +4 more
openaire +2 more sources