Results 71 to 80 of about 526,264 (266)

Multiplex SNP Discrimination [PDF]

Biophysical Journal, 2007
Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately
Fish, Daniel J., Horne, M. Todd, Searles, Robert P., Brewood, Greg P., Benight, Albert S.   +4 more
openaire   +3 more sources

Monitoring of circulating tumor DNA allows early detection of disease relapse in patients with operable breast cancer

Molecular Oncology, EarlyView.
Monitoring circulating tumor DNA (ctDNA) in patients with operable breast cancer can reveal disease relapse earlier than radiology in a subset of patients. The failure to detect ctDNA in some patients with recurrent disease suggests that ctDNA could serve as a supplement to other monitoring approaches.
Kristin Løge Aanestad, Marie Austdal, Oddmund Nordgård, Gunnar Mellgren, Satu Oltedal, Marie L. Austbø, Ylva H. Vignes, Thomas Helland, Kristin Jonsdottir, Tone H. Lende, Emilius A. M. Janssen, Bjørnar Gilje, Kjersti Tjensvoll, PBCB study group, Gunnar Mellgren, Tone Hoel Lende, Anette Heie, Kristin Viste, Anita Røyneberg Alvheim, Emiel AM Janssen, Kristin Jonsdottir, Ann Cathrine Kroksveen, Thomas Helland, Einar Gudlaugsson, Oddmund Nordgård, Satu Oltedal, Kristin Løge Aanestad, Jan Terje Kvaløy, Kirsten Lode, Kari Britt Hagen, Marie Austdal, Ylva H. Vignes, Siri Tungland Sola, Nina Egeland Amundsen, Finn Magnus Eliassen, Emeritus Ernst A Lien   +35 more
wiley   +1 more source

Crucial parameters for precise copy number variation detection in formalin‐fixed paraffin‐embedded solid cancer samples

Molecular Oncology, EarlyView.
This study shows that copy number variations (CNVs) can be reliably detected in formalin‐fixed paraffin‐embedded (FFPE) solid cancer samples using ultra‐low‐pass whole‐genome sequencing, provided that key (pre)‐analytical parameters are optimized.
Hanne Goris, Vasiliki Siozopoulou, Léon C van Kempen, Anne Sieben, Ella Roelant, Stig Hellemans, Elyne Backx, Laure Sorber, Koen De Winne, Senada Koljenović, Karen Zwaenepoel   +10 more
wiley   +1 more source

Isolation, genetic identification of Amazonian yeasts and analysis of thermotolerance and alcohol tolerance of Saccharomyces cerevisiae from Theobroma grandiflorum and Eugenia stipitata

AIMS Bioengineering
Although yeasts of the Saccharomyces cerevisiae species are industrially significant, few studies have investigated their presence in environmental samples from the Amazon rainforest. This study aimed to isolate S. cerevisiae yeasts associated with trees
Flávia da Silva Fernandes , Luan Reis Honorato da Silva, Érica Simplício de Souza , Lívia Melo Carneiro, João Paulo Alves Silva, Steven Zelski , João Vicente Braga de Souza, Jacqueline da Silva Batista   +7 more
doaj   +1 more source

Long cryopreserved lab-adapted Plasmodium falciparum increases resistance to chloroquine but not its susceptibility

Life Sciences, Medicine and Biomedicine, 2020
Introduction: Plasmodium falciparum (P. falciparum) is a deadly protozoan that is accountable for malaria and chloroquine was the first-line antimalarial drug before its withdrawal and replaced by artemisinin.
Jing Yit Pua, Nurhidanatasha Abu Bakar, Nik Abdul Aziz Nik Kamarudin, Siti Zulaiha Ghazali, Khairul Mohd Fadzli Mustaffa   +4 more
doaj   +1 more source

Genomic dissection of the 1994 Cronobacter sakazakii outbreak in a French neonatal intensive care unit [PDF]

, 2015
Background: Cronobacter sakazakii is a member of the genus Cronobacter that has frequently been isolated from powdered infant formula (PIF) and linked with rare but fatal neonatal infections such as meningitis and necrotising enterocolitis.
Dickins, B, Farbos, A, Forsythe, S, Masood, N, McNally, A, Moore, K, Paszkiewicz, K   +6 more
core   +2 more sources

SNP-o-matic [PDF]

Bioinformatics, 2009
Abstract Motivation: High throughput sequencing technologies generate large amounts of short reads. Mapping these to a reference sequence consumes large amounts of processing time and memory, and read mapping errors can lead to noisy or incorrect alignments. SNP-o-matic is a fast, memory-efficient and stringent read mapping tool offering
Manske, H, Kwiatkowski, D
openaire   +3 more sources

Genetic attenuation of ALDH1A1 increases metastatic potential and aggressiveness in colorectal cancer

Molecular Oncology, EarlyView.
Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell marker in several malignancies. We established a novel epithelial cell line from rectal adenocarcinoma with unique overexpression of this enzyme. Genetic attenuation of ALDH1A1 led to increased invasive capacity and metastatic potential, the inhibition of proliferation activity, and ultimately ...
Martina Poturnajova, Zuzana Kozovska, Ondrej Pos, Kristina Pavlov, Sachin Gulati, Peter Makovicky, Kristina Jakic, Monika Burikova, Eva Sedlackova, Barbora Svitkova, Silvia Tyciakova, Vojtech Bystry, Nicolas Blavet, Boris Tichy, Matej Hrnciar, Jaroslav Budis, Miroslav Tomas, Peter Dubovan, Georgina Kolnikova, Veronika Repaska, Nikoleta Mojzesova, Eva Zomborska, Daniel Pindak, Michal Mego, Tomas Szemes, Miroslava Matuskova   +25 more
wiley   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

The revelation of genomic breed composition using target capture sequencing: a case of Taxodium

Forestry Research
Taxodium plants have good flood tolerance and thus were introduced into China from North America in the early 1900s. The subsequent decades of cross-breeding experiments within Taxodium have produced many new hybrid cultivars in China while also creating
Zhitong Han, Yangkang Chen, Xiaogang Dai, Chaoguang Yu, Jiajin Cheng, Jialiang Li, Kangshan Mao   +6 more
doaj   +1 more source