Results 21 to 30 of about 17,437 (131)

Integrating spatial transcriptomics and snRNA-seq data enhances differential gene expression analysis results of AD-related phenotypes [PDF]

Human Genetics and Genomics Advances
Shizhen Tang, Jingjing Yang, Aron S Buchman   +2 more
exaly   +2 more sources

Comprehensive analyses of brain cell communications based on multiple scRNA-seq and snRNA-seq datasets for revealing novel mechanism in neurodegenerative diseases [PDF]

CNS Neuroscience and Therapeutics, 2023
Chunlong Zhang, Yunpeng Zhang, Guiyuan Tan   +2 more
exaly   +2 more sources

Single-nucleus RNA sequencing of human pancreatic islets identifies novel gene sets and distinguishes β-cell subpopulations with dynamic transcriptome profiles

Genome Medicine, 2023
Background Single-cell RNA sequencing (scRNA-seq) provides valuable insights into human islet cell types and their corresponding stable gene expression profiles. However, this approach requires cell dissociation that complicates its utility in vivo.
Randy B. Kang, Yansui Li, Carolina Rosselot, Tuo Zhang, Mustafa Siddiq, Prashant Rajbhandari, Andrew F. Stewart, Donald K. Scott, Adolfo Garcia-Ocana, Geming Lu   +9 more
doaj   +1 more source

Isolation of Nuclei from Human Snap-frozen Liver Tissue for Single-nucleus RNA Sequencing

Bio-Protocol, 2023
Single-nucleus RNA sequencing (snRNA-seq) provides a powerful tool for studying cell type composition in heterogenous tissues. The liver is a vital organ composed of a diverse set of cell types; thus, single-cell technologies could greatly facilitate the
Marcus Alvarez, Jihane Benhammou, Shuyun Rao, Lopa Mishra, Joseph Pisegna, Päivi Pajukanta   +5 more
doaj   +1 more source

Function and Development of Deep-sea Mussel Bacteriocytes Revealed by snRNA-seq and Spatial Transcriptomics. [PDF]

Genomics Proteomics Bioinformatics
Abstract Deep-sea chemosynthetic ecosystems are among the most unusual ecosystems on Earth, where most megafauna form close symbiotic associations with chemosynthetic microbes to obtain nutrition and shelter from the toxic environment.
Chen 陈浩 H, Li 李梦娜 M, Zhong 钟兆山 Z, Seim I, Wang 王敏晓 M, Lian 连超 C, Zhuo 卓联鸿 L, Wan 宛新江 X, Wang 王昊 H, Han 韩广辉 G, Zhou 周丽 L, Zhang 张峘 H, Cao 曹磊 L, Li 李超伦 C.   +13 more
europepmc   +3 more sources

snRNA-Seq and Spatial Transcriptome Reveal Cell–Cell Crosstalk Mediated Metabolic Regulation in Porcine Skeletal Muscle [PDF]

Journal of Cachexia, Sarcopenia and Muscle
Fengna Li
exaly   +2 more sources

Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics. [PDF]

STAR Protoc
The silk glands (SGs) of silkworms specifically synthesize silk proteins, thus strongly influencing the yield and quality of silk. Here, we present a protocol for isolating SG nuclei from silkworms and obtaining high-quality tissue slices for spatial transcriptomics. We describe steps for rearing, dissecting, and nucleus isolation.
Ma Y, Dong K, Hu J, Tang Y, Xu H.
europepmc   +4 more sources

High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seq

Nature Communications, 2023
Formalin-fixed paraffin-embedded (FFPE) tissues constitute a vast and valuable patient material bank for clinical history and follow-up data. It is still challenging to achieve single cell/nucleus RNA (sc/snRNA) profile in FFPE tissues.
Ziye Xu, Tianyu Zhang, Hongyu Chen, Yuyi Zhu, Yuexiao Lv, Shunji Zhang, Jiaye Chen, Haide Chen, Lili Yang, Weiqin Jiang, Shengyu Ni, Fangru Lu, Zhaolun Wang, Hao Yang, Ling Dong, Feng Chen, Hong Zhang, Yu Chen, Jiong Liu, Dandan Zhang, Longjiang Fan, Guoji Guo, Yongcheng Wang   +22 more
doaj   +1 more source

Use of scREAD to explore and analyze single-cell and single-nucleus RNA-seq data for Alzheimer’s disease

STAR Protocols, 2021
Summary: Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease.
Cankun Wang, Yujia Xiang, Hongjun Fu, Qin Ma   +3 more
doaj   +1 more source

Neuron-specific transcriptomic dysregulation in methotrexate-induced cognitive impairment revealed by SnRNA-seq. [PDF]

Int J Surg
Background: Methotrexate (MTX) is a widely used chemotherapy drug, but its neurotoxicity can lead to cognitive impairments, particularly through effects on hippocampal function. Nevertheless, the underlying molecular mechanisms are not fully understood.
Ye J, Ding Y, Wu R, Ding H, Ren J, Xia Z, Chen J, Xie S, Jia Y.   +8 more
europepmc   +3 more sources