Results 31 to 40 of about 15,972 (195)

Isolation of Nuclei from Human Snap-frozen Liver Tissue for Single-nucleus RNA Sequencing

Bio-Protocol, 2023
Single-nucleus RNA sequencing (snRNA-seq) provides a powerful tool for studying cell type composition in heterogenous tissues. The liver is a vital organ composed of a diverse set of cell types; thus, single-cell technologies could greatly facilitate the
Marcus Alvarez, Jihane Benhammou, Shuyun Rao, Lopa Mishra, Joseph Pisegna, Päivi Pajukanta   +5 more
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Use of scREAD to explore and analyze single-cell and single-nucleus RNA-seq data for Alzheimer’s disease

STAR Protocols, 2021
Summary: Single-cell RNA-sequencing (scRNA-seq) and single-nucleus RNA-sequencing (snRNA-seq) studies have provided remarkable insights into understanding the molecular pathogenesis of Alzheimer's disease.
Cankun Wang, Yujia Xiang, Hongjun Fu, Qin Ma   +3 more
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High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seq

Nature Communications, 2023
Formalin-fixed paraffin-embedded (FFPE) tissues constitute a vast and valuable patient material bank for clinical history and follow-up data. It is still challenging to achieve single cell/nucleus RNA (sc/snRNA) profile in FFPE tissues.
Ziye Xu, Tianyu Zhang, Hongyu Chen, Yuyi Zhu, Yuexiao Lv, Shunji Zhang, Jiaye Chen, Haide Chen, Lili Yang, Weiqin Jiang, Shengyu Ni, Fangru Lu, Zhaolun Wang, Hao Yang, Ling Dong, Feng Chen, Hong Zhang, Yu Chen, Jiong Liu, Dandan Zhang, Longjiang Fan, Guoji Guo, Yongcheng Wang   +22 more
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Improved protocol for single-nucleus RNA-sequencing of frozen human bladder tumor biopsies

Nucleus, 2023
This paper provides a laboratory workflow for single-nucleus RNA-sequencing (snRNA-seq) including a protocol for gentle nuclei isolation from fresh frozen tumor biopsies, making it possible to analyze biobanked material. To develop this protocol, we used
Sofie S. Schmøkel, Iver Nordentoft, Sia V. Lindskrog, Philippe Lamy, Michael Knudsen, Jørgen Bjerggaard Jensen, Lars Dyrskjøt   +6 more
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Nuclei isolation from surgically resected human hippocampus

STAR Protocols, 2021
Summary: Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain.
Fatma Ayhan, Connor Douglas, Bradley C. Lega, Genevieve Konopka   +3 more
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Single-Cell RNA Sequencing in Parkinson’s Disease

Biomedicines, 2021
Single-cell and single-nucleus RNA sequencing (sc/snRNA-seq) technologies have enhanced the understanding of the molecular pathogenesis of neurodegenerative disorders, including Parkinson’s disease (PD).
Shi-Xun Ma, Su Bin Lim
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Integration of single-nuclei RNA-sequencing, spatial transcriptomics and histochemistry defines the complex microenvironment of NF1-associated plexiform neurofibromas

Acta Neuropathologica Communications, 2023
Plexiform neurofibroma (PN) is a leading cause of morbidity in children with the genetic condition Neurofibromatosis Type 1 (NF1), often disfiguring or threatening vital structures. During formation of PN, a complex tumor microenvironment (TME) develops,
Vladimir Amani, Kent A. Riemondy, Rui Fu, Andrea M. Griesinger, Enrique Grimaldo, Graziella Ribeiro De Sousa, Ahmed Gilani, Molly Hemenway, Nicholas K. Foreman, Andrew M. Donson, Nicholas Willard   +10 more
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SCING: Inference of robust, interpretable gene regulatory networks from single cell and spatial transcriptomics

iScience, 2023
Summary: Gene regulatory network (GRN) inference is an integral part of understanding physiology and disease. Single cell/nuclei RNA-seq (scRNA-seq/snRNA-seq) data has been used to elucidate cell-type GRNs; however, the accuracy and speed of current ...
Russell Littman, Michael Cheng, Ning Wang, Chao Peng, Xia Yang   +4 more
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Single‐Cell, Single‐Nucleus, and Spatial RNA Sequencing of the Human Liver Identifies Cholangiocyte and Mesenchymal Heterogeneity

Hepatology Communications, 2022
The critical functions of the human liver are coordinated through the interactions of hepatic parenchymal and non‐parenchymal cells. Recent advances in single‐cell transcriptional approaches have enabled an examination of the human liver with ...
Tallulah S. Andrews, Jawairia Atif, Jeff C. Liu, Catia T. Perciani, Xue‐Zhong Ma, Cornelia Thoeni, Michal Slyper, Gökcen Eraslan, Asa Segerstolpe, Justin Manuel, Sai Chung, Erin Winter, Iulia Cirlan, Nicholas Khuu, Sandra Fischer, Orit Rozenblatt‐Rosen, Aviv Regev, Ian D. McGilvray, Gary D. Bader, Sonya A. MacParland   +19 more
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Single-nucleus and single-cell transcriptomes compared in matched cortical cell types.

PLoS ONE, 2018
Transcriptomic profiling of complex tissues by single-nucleus RNA-sequencing (snRNA-seq) affords some advantages over single-cell RNA-sequencing (scRNA-seq). snRNA-seq provides less biased cellular coverage, does not appear to suffer cell isolation-based
Trygve E Bakken, Rebecca D Hodge, Jeremy A Miller, Zizhen Yao, Thuc Nghi Nguyen, Brian Aevermann, Eliza Barkan, Darren Bertagnolli, Tamara Casper, Nick Dee, Emma Garren, Jeff Goldy, Lucas T Graybuck, Matthew Kroll, Roger S Lasken, Kanan Lathia, Sheana Parry, Christine Rimorin, Richard H Scheuermann, Nicholas J Schork, Soraya I Shehata, Michael Tieu, John W Phillips, Amy Bernard, Kimberly A Smith, Hongkui Zeng, Ed S Lein, Bosiljka Tasic   +27 more
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