Results 51 to 60 of about 1,857,199 (369)
Real‐time assay of ribonucleotide reductase activity with a fluorescent RNA aptamer
FEBS Letters, EarlyView.Ribonucleotide reductases (RNR) synthesize DNA building blocks de novo, making them crucial in DNA replication and drug targeting. FLARE introduces the first single‐tube real‐time coupled RNR assay, which enables isothermal tracking of RNR activity at nanomolar enzyme levels and allows the reconstruction of allosteric regulatory patterns and rapid ...
Jacopo De Capitani +4 more
wiley +1 more source
Organic Synthesis on Solid Phase. (Supports, Linkers, Reactions)
Molecules, 2001 n ...
Florencio Zaragoza Dörwald
doaj +1 more source
Solid-Phase Synthesis of Megamolecules
Journal of the American Chemical Society, 2020 This paper presents a solid-phase strategy to efficiently assemble multiprotein scaffolds-known as megamolecules-without the need for protecting groups and with precisely defined nanoscale architectures. The megamolecules are assembled through sequential reactions of linkers that present irreversible inhibitors for enzymes and fusion proteins ...
Blaise R. Kimmel +4 more
openaire +3 more sources
Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions
FEBS Letters, EarlyView.We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva +10 more
wiley +1 more source
Solid Phase Synthesis of a Metronidazole Oligonucleotide Conjugate
Molecules, 2006 Direct, solid phase synthesis of an oligonucleotide conjugate of the antibioticdrug metronidazole was accomplished by the phosphoramidite method. Removal ofprotecting groups and cleavage from the controlled pore glass (CPG) solid support wassuccessful ...
William Fraser +2 more
doaj +1 more source
Advances in Fmoc solid‐phase peptide synthesis
Journal of Peptide Science, 2016 Today, Fmoc SPPS is the method of choice for peptide synthesis. Very‐high‐quality Fmoc building blocks are available at low cost because of the economies of scale arising from current multiton production of therapeutic peptides by Fmoc SPPS.
R. Behrendt, P. White, J. Offer
semanticscholar +1 more source
Cyclative cleavage via solid-phase supported stabilized sulfur ylides: synthesis of macrocyclic lactones [PDF]
, 2002 A new synthesis of macrolactones bearing a cyclopropyl ring condensed to the macrocycle is reported via a cyclization-release strategy making use of solid-phase supported stabilized sulfur ...
CARDULLO F +6 more
core +1 more source
FEBS Letters, EarlyView.The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li +2 more
wiley +1 more source
CHIMIA, 1996 In this account dedicated to '100 years Roche' in CHIMIA, we present some of our strategies towards the synthesis of interesting novel amino-acid-derived building blocks; multigeneration synthesis of thiazole libraries in solution; a novel solid-
Alexander Chucholowski +4 more
doaj +2 more sources
Fast microwave-assisted synthesis of Li-stuffed garnets and insights into Li diffusion from muon spin spectroscopy [PDF]
, 2016 Lithium-stuffed garnets attract huge attention due to their outstanding potential as solid-state electrolytes for lithium batteries. However, there exists a persistent challenge in the reliable synthesis of these complex functional oxides together with a
Ahston, Thomas E. +4 more
core +1 more source