Dysregulated biosynthesis and hydrolysis of cyclic-di-adenosine monophosphate impedes sporulation and butanol and acetone production in Clostridium beijerinckii NCIMB 8052 [PDF]
Frontiers in Bioengineering and BiotechnologyIntroductionAlthough solventogenic Clostridium species (SCS) produce butanol, achieving high enough titers to warrant commercialization of biobutanol remains elusive. Thus, deepening our understanding of the intricate cellular wiring of SCS is crucial to
Marian M. Awaga-Cromwell +5 more
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Genome-wide dynamic transcriptional profiling in
BMC Genomics, 2012 Background Clostridium beijerinckii is a prominent solvent-producing microbe that has great potential for biofuel and chemical industries. Although transcriptional analysis is essential to understand gene functions and regulation and thus elucidate ...
Wang Yi +3 more
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From pre-culture to solvent: current trends in Clostridium acetobutylicum cultivation [PDF]
Applied Microbiology and BiotechnologyThe biological production of butanol via ABE (acetone-butanol-ethanol) fermentation using Clostridium acetobutylicum has a storied history of over 100 years, initially driven by the demand for synthetic rubber during World War I and later for industrial ...
Katharina Oehlenschläger +3 more
doaj +2 more sources
Metabolic Engineering of Histidine Kinases in Clostridium beijerinckii for Enhanced Butanol Production [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 Clostridium beijerinckii, a promising industrial microorganism for butanol production, suffers from low butanol titer and lack of high-efficiency genetical engineering toolkit. A few histidine kinases (HKs) responsible for Spo0A phosphorylation have been
Xin Xin +4 more
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Applied Microbiology and Biotechnology, 2014 The fermentation carried out by the solvent-producing bacterium, Clostridium acetobutylicum, is characterized by two distinct phases: acidogenic and solventogenic phases. Understanding the cellular physiological changes occurring during the phase transition in clostridial fermentation is important for the enhanced production of solvents.
Jang, Yu-Sin +7 more
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A CRISPR/Anti-CRISPR Genome Editing Approach Underlines the Synergy of Butanol Dehydrogenases in Clostridium acetobutylicum DSM 792 [PDF]
Appl Environ Microbiol, 2020 International audienceAlthough Clostridium acetobutylicum is the model organism for the study of acetone-butanol-ethanol (ABE) fermentation, its characterization has long been impeded by the lack of efficient genome editing tools.
Chartier, Gwladys +3 more
core +2 more sources
Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation [PDF]
Biotechnology for Biofuels and BioproductsBackground Inhibitors that are released from lignocellulose biomass during its treatment represent one of the major bottlenecks hindering its massive utilization in the biotechnological production of chemicals.
Barbora Branska +3 more
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Homoacetogenesis and solventogenesis from H2/CO2 by granular sludge at 25, 37 and 55 °C
Chemosphere, 2021 CO2 fermentation is a promising process to produce biofuels like ethanol. It can be integrated in third generation biofuel production processes to substitute traditional sugar fermentation when supplied with cheap electron donors, e.g. hydrogen derived from wind energy or as surplus gas in electrolysis. In this study, granular sludge from an industrial
Chiara Cassarini +3 more
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ChemElectroChem, Volume 10, Issue 21, November 2, 2023., 2023 A bioelectrochemical system based on a stirred tank bioreactor with a rotating carbon fiber brush as working electrode (WE) and stirrer was developed. The three‐electrode system was closed with a carbon felt counter electrode (CE) and an Ag/AgCl (3 M KCl) reference electrode (RE). Initial abiotic experiments and a biotic proof of concept via a cathodic
Jan‐Niklas Hengsbach +6 more
wiley +1 more source
mRNA analysis of the adc gene region of Clostridium acetobutylicum during the shift to solventogenesis [PDF]
Journal of Bacteriology, 1992 By using primer extension analysis, we located the transcription start point of the acetoacetate decarboxylase (adc) gene of Clostridium acetobutylicum 90 nucleotides upstream from the initiation codon with A as the first transcribed nucleotide. From this site the promoter structure TTTACT(18 bp)TATAAT was identified; it shows high homology to the ...
Gerischer, Ulrike, Dürre, Peter
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