Results 11 to 20 of about 267,878 (327)

Epigenetic Reprogramming During Somatic Cell Nuclear Transfer: Recent Progress and Future Directions [PDF]

Frontiers in Genetics, 2020
Somatic cell nuclear transfer (SCNT) has broad applications but is limited by low cloning efficiency. In this review, we mainly focus on SCNT-mediated epigenetic reprogramming in livestock and also describe mice data for reference.
Xiangyu Wang, Jiadan Qu, Jie Li, Hongbin He, Zhonghua Liu, Yanjun Huan   +5 more
doaj   +2 more sources

Somatic cell nuclear transfer [PDF]

Nature, 2002
Cloning by nuclear transfer from adult somatic cells is a remarkable demonstration of developmental plasticity. When a nucleus is placed in oocyte cytoplasm, the changes in chromatin structure that govern differentiation can be reversed, and the nucleus can be made to control development to term.
Tim King, P.A. De Sousa, Nathalie Beaujean, Lorraine E. Young, Lorraine E. Young, D.N. Wells, L A Paterson, Andras Dinnyes, Andras Dinnyes, Ian Wilmut   +9 more
  +9 more sources

Interspecific Nuclear Transfer Blastocysts Reconstructed from Arabian Oryx Somatic Cells and Domestic Cow Ooplasm

Veterinary Sciences, 2022
Cloning, commonly referred to as somatic cell nuclear transfer (SCNT), is the technique of enucleating an oocyte and injecting a somatic cell into it. This study was carried out with interspecific SCNT technology to clone the Arabian Oryx utilizing the ...
Aiman A. Ammari, Muath G. ALGhadi, Ramzi A. Amran, Nawal M. Al Malahi, Ahmad R. Alhimaidi   +4 more
doaj   +1 more source

Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer

Journal of Animal Reproduction and Biotechnology, 2022
Haploidization in somatic cells is the process of reducing the diploid somatic chromosomes to haploid. Several studies have attempted somatic haploidization using oocytes in mice and humans.
Yeonmi Lee , Eunju Kang
doaj   +1 more source

Mitosis gives a brief window of opportunity for a change in gene transcription. [PDF]

PLoS Biology, 2014
Cell differentiation is remarkably stable but can be reversed by somatic cell nuclear transfer, cell fusion, and iPS. Nuclear transfer to amphibian oocytes provides a special opportunity to test transcriptional reprogramming without cell division.
Richard P Halley-Stott, Jerome Jullien, Vincent Pasque, John Gurdon   +3 more
doaj   +1 more source

The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos [PDF]

Macedonian Veterinary Review, 2016
One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus.
Martin Morovic, Matej Murin, Frantisek Strejcek, Michal Benc, Dusan Paál, Olga Østrup, Heiner Niemann, Lazo Pendovski, Jozef Laurincik   +8 more
doaj   +1 more source

Correction: Reprogrammed Transcriptome in Rhesus-Bovine Interspecies Somatic Cell Nuclear Transfer Embryos.

PLoS ONE, 2012
Kai Wang, Hasan H. Otu, Ying Chen, Young Lee, Keith Latham, Jose B. Cibelli   +5 more
doaj   +5 more sources

The combination of rolipram and cilostamide improved the developmental competence of cloned porcine embryos

Scientific Reports, 2023
In vitro maturation of porcine oocytes is characterized by asynchronous cytoplasmic and nuclear maturation, leading to less competent oocytes supporting embryo development.
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Chaerim Seo, Heejae Kang, Dabin Cha, Ahmad Yar Qamar, Joohyun Shim, Kimyung Choi, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho   +11 more
doaj   +1 more source

NUCLEAR REPROGRAMMING AND ITS EPIGENETIC CONSEQUENCES IN SOMATIC CELL NUCLEAR TRANSFER DERIVED ANIMALS [PDF]

SPERMOVA, 2015
Somatic cell nuclear transfer (SCNT) is a powerful tool to understand the mechanisms driving the cellular reprogramming, as well as addressing fundamental questions on developmental biology.
Meirelles FV, Sampaio RV, da Silveira JC, Sangalli JR, Fantinato-Neto P, Birgel Jr. E, Bressan F, Perecin F   +7 more
doaj   +1 more source

Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos. [PDF]

PLoS ONE, 2011
Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive.In this study, we analyzed the
Kai Wang, Hasan H Otu, Ying Chen, Young Lee, Keith Latham, Jose B Cibelli   +5 more
doaj   +1 more source