Results 21 to 30 of about 267,878 (327)
PLoS ONE, 2014 Somatic cell nuclear transfer is used to generate genetic models for research and new, genetically modified livestock varieties. Goat fetal fibroblast cells (gFFCs) are the predominant nuclear donors in Cashmere goat transgenic cloning, but have ...
Yu Ren +5 more
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Factors influencing the somatic cell nuclear transfer efficiency in pigs [PDF]
Frontiers of Agricultural Science and Engineering, 2019 Using a data set from our laboratory, we assessed the effects of several factors on pig cloning efficiency. The results demonstrated that cells at high confluence (>90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall ...
Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI
doaj +1 more source
Epigenetic abnormalities associated with somatic cell nuclear transfer [PDF]
Reproduction, 2021 Twenty-five years have passed since the birth of Dolly the sheep, the first mammalian clone produced by adult somatic cell nuclear transfer (SCNT). During that time, the main thrust of SCNT-related research has been the elucidation of SCNT-associated epigenetic abnormalities and their correction, with the aim of improving the efficiency of cloned ...
Atsuo Ogura, Kimiko Inoue, Shogo Matoba
openaire +2 more sources
Cloning animals by somatic cell nuclear transfer – biological factors
Reproductive Biology and Endocrinology, 2003 Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced.
Enright Brian +3 more
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RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming. [PDF]
PLoS ONE, 2016 The specific molecular mechanisms involved in somatic reprogramming remain unidentified. Removal of the oocyte genome is one of the primary causes of developmental failure in cloned embryos, whereas intact oocyte shows stronger reprogramming capability ...
Lin Bai +6 more
doaj +1 more source
Generation of bovine transgenics using somatic cell nuclear transfer
Reproductive Biology and Endocrinology, 2003 The ability to produce transgenic animals through the introduction of exogenous DNA has existed for many years. However, past methods available to generate transgenic animals, such as pronuclear microinjection or the use of embryonic stem cells, have ...
Stice Steven L, Hodges Craig A
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Influences of somatic donor cell sex on and embryo development following somatic cell nuclear transfer in pigs [PDF]
Asian-Australasian Journal of Animal Sciences, 2017 Objective The present study investigates pre- and post-implantation developmental competence of nuclear-transferred porcine embryos derived from male and female fetal fibroblasts.
Jae-Gyu Yoo +9 more
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Single-Molecule Imaging of Nav1.6 on the Surface of Hippocampal Neurons Reveals Somatic Nanoclusters [PDF]
Biophysical Journal, Vol. 111, Issue 6, 1235-1247 (2016), 2016 Voltage-gated sodium (Na$_\mathrm{v}$) channels are responsible for the depolarizing phase of the action potential in most nerve cells, and Na$_\mathrm{v}$ channel localization to the axon initial segment is vital to action potential initiation. Na$_\mathrm{v}$ channels in the soma play a role in the transfer of axonal output information to the rest of
arxiv +1 more source
BMC Genomics, 2018 Background Nuclear reprogramming reinstates totipotency or pluripotency in somatic cells by changing their gene transcription profile. This technology is widely used in medicine, animal husbandry and other industries.
Yong Liu +13 more
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Journal of Applied Clinical Medical Physics, Volume 23, Issue 12, December 2022., 2022 Abstract Positron emission tomography with x‐ray computed tomography (PET/CT) is increasingly being utilized for radiation treatment planning (RTP). Accurate delivery of RT therefore depends on quality PET/CT data. This study covers quality control (QC) procedures required for PET/CT for diagnostic imaging and incremental QC required for RTP.
Ran Klein +7 more
wiley +1 more source