Results 21 to 30 of about 63,552 (337)
Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer
Journal of Animal Reproduction and Biotechnology, 2022 Haploidization in somatic cells is the process of reducing the diploid somatic chromosomes to haploid. Several studies have attempted somatic haploidization using oocytes in mice and humans.
Yeonmi Lee , Eunju Kang
doaj +1 more source
The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos [PDF]
Macedonian Veterinary Review, 2016 One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus.
Martin Morovic +8 more
doaj +1 more source
Scientific Reports, 2023 In vitro maturation of porcine oocytes is characterized by asynchronous cytoplasmic and nuclear maturation, leading to less competent oocytes supporting embryo development.
Bereket Molla Tanga +11 more
doaj +1 more source
NUCLEAR REPROGRAMMING AND ITS EPIGENETIC CONSEQUENCES IN SOMATIC CELL NUCLEAR TRANSFER DERIVED ANIMALS [PDF]
SPERMOVA, 2015 Somatic cell nuclear transfer (SCNT) is a powerful tool to understand the mechanisms driving the cellular reprogramming, as well as addressing fundamental questions on developmental biology.
Meirelles FV +7 more
doaj +1 more source
Reprogrammed transcriptome in rhesus-bovine interspecies somatic cell nuclear transfer embryos. [PDF]
PLoS ONE, 2011 Global activation of the embryonic genome (EGA), one of the most critical steps in early mammalian embryo development, is recognized as the time when interspecies somatic cell nuclear transfer (iSCNT) embryos fail to thrive.In this study, we analyzed the
Kai Wang +5 more
doaj +1 more source
PLoS ONE, 2014 Somatic cell nuclear transfer is used to generate genetic models for research and new, genetically modified livestock varieties. Goat fetal fibroblast cells (gFFCs) are the predominant nuclear donors in Cashmere goat transgenic cloning, but have ...
Yu Ren +5 more
doaj +1 more source
Epigenetic abnormalities associated with somatic cell nuclear transfer [PDF]
Reproduction, 2021 Twenty-five years have passed since the birth of Dolly the sheep, the first mammalian clone produced by adult somatic cell nuclear transfer (SCNT). During that time, the main thrust of SCNT-related research has been the elucidation of SCNT-associated epigenetic abnormalities and their correction, with the aim of improving the efficiency of cloned ...
Atsuo Ogura, Kimiko Inoue, Shogo Matoba
openaire +2 more sources
Factors influencing the somatic cell nuclear transfer efficiency in pigs [PDF]
Frontiers of Agricultural Science and Engineering, 2019 Using a data set from our laboratory, we assessed the effects of several factors on pig cloning efficiency. The results demonstrated that cells at high confluence (>90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall ...
Yong JIN, Manling ZHANG, Xinrong JU, Shuang LIANG, Qiang XIONG, Lihua ZHAO, Xiaowei NIE, Daorong HOU, Qiang LIU, Junzheng WANG, Chenyu WANG, Xiaokang LI, Lining ZHANG, Xiaorui LIU, Ying WANG, Haiyuan YANG, Yifan DAI, Rongfeng LI
doaj +1 more source
Cloning animals by somatic cell nuclear transfer – biological factors
Reproductive Biology and Endocrinology, 2003 Cloning by nuclear transfer using mammalian somatic cells has enormous potential application. However, somatic cloning has been inefficient in all species in which live clones have been produced.
Enright Brian +3 more
doaj +1 more source