Results 21 to 30 of about 67,513 (334)

Integrating Transcriptomics, Proteomics, and Metabolomics to Investigate the Mechanism of Fetal Placental Overgrowth in Somatic Cell Nuclear Transfer Cattle. [PDF]

Int J Mol Sci
Zhao X, Wu S, Yun Y, Du Z, Liu S, Bo C, Gao Y, Yang L, Song L, Bai C, Su G, Li G.   +11 more
europepmc   +3 more sources

Interspecific Nuclear Transfer Blastocysts Reconstructed from Arabian Oryx Somatic Cells and Domestic Cow Ooplasm

Veterinary Sciences, 2022
Cloning, commonly referred to as somatic cell nuclear transfer (SCNT), is the technique of enucleating an oocyte and injecting a somatic cell into it. This study was carried out with interspecific SCNT technology to clone the Arabian Oryx utilizing the ...
Aiman A. Ammari, Muath G. ALGhadi, Ramzi A. Amran, Nawal M. Al Malahi, Ahmad R. Alhimaidi   +4 more
doaj   +1 more source

Haploidy of somatic cells in mouse oocyte using somatic cell nuclear transfer

Journal of Animal Reproduction and Biotechnology, 2022
Haploidization in somatic cells is the process of reducing the diploid somatic chromosomes to haploid. Several studies have attempted somatic haploidization using oocytes in mice and humans.
Yeonmi Lee , Eunju Kang
doaj   +1 more source

The influence of interspecies somatic cell nuclear transfer on epigenetic enzymes transcription in early embryos [PDF]

Macedonian Veterinary Review, 2016
One of the main reason for the incorrect development of embryos derived from somatic cell nuclear transfer is caused by insufficient demethylation of injected somatic chromatin to a state comparable with an early embryonic nucleus.
Martin Morovic, Matej Murin, Frantisek Strejcek, Michal Benc, Dusan Paál, Olga Østrup, Heiner Niemann, Lazo Pendovski, Jozef Laurincik   +8 more
doaj   +1 more source

Epigenetic Reprogramming During Somatic Cell Nuclear Transfer: Recent Progress and Future Directions

Frontiers in Genetics, 2020
Somatic cell nuclear transfer (SCNT) has broad applications but is limited by low cloning efficiency. In this review, we mainly focus on SCNT-mediated epigenetic reprogramming in livestock and also describe mice data for reference.
Xiangyu Wang, Jiadan Qu, J. Li, Hongbin He, Zhonghua Liu, Yanjun Huan   +5 more
semanticscholar   +1 more source

The combination of rolipram and cilostamide improved the developmental competence of cloned porcine embryos

Scientific Reports, 2023
In vitro maturation of porcine oocytes is characterized by asynchronous cytoplasmic and nuclear maturation, leading to less competent oocytes supporting embryo development.
Bereket Molla Tanga, Xun Fang, Seonggyu Bang, Chaerim Seo, Heejae Kang, Dabin Cha, Ahmad Yar Qamar, Joohyun Shim, Kimyung Choi, Islam M. Saadeldin, Sanghoon Lee, Jongki Cho   +11 more
doaj   +1 more source

NUCLEAR REPROGRAMMING AND ITS EPIGENETIC CONSEQUENCES IN SOMATIC CELL NUCLEAR TRANSFER DERIVED ANIMALS [PDF]

SPERMOVA, 2015
Somatic cell nuclear transfer (SCNT) is a powerful tool to understand the mechanisms driving the cellular reprogramming, as well as addressing fundamental questions on developmental biology.
Meirelles FV, Sampaio RV, da Silveira JC, Sangalli JR, Fantinato-Neto P, Birgel Jr. E, Bressan F, Perecin F   +7 more
doaj   +1 more source

Potential of adipose-derived mesenchymal stem cells and skeletal muscle-derived satellite cells for somatic cell nuclear transfer mediated transgenesis in Arbas Cashmere goats.

PLoS ONE, 2014
Somatic cell nuclear transfer is used to generate genetic models for research and new, genetically modified livestock varieties. Goat fetal fibroblast cells (gFFCs) are the predominant nuclear donors in Cashmere goat transgenic cloning, but have ...
Yu Ren, Haiqing Wu, Yuzhen Ma, Jianlong Yuan, Hao Liang, Dongjun Liu   +5 more
doaj   +1 more source

RNA-Seq Profiling of Intact and Enucleated Oocyte SCNT Embryos Reveals the Role of Pig Oocyte Nucleus in Somatic Reprogramming. [PDF]

PLoS ONE, 2016
The specific molecular mechanisms involved in somatic reprogramming remain unidentified. Removal of the oocyte genome is one of the primary causes of developmental failure in cloned embryos, whereas intact oocyte shows stronger reprogramming capability ...
Lin Bai, Mengqi Li, Junli Sun, Xiaogan Yang, Yangqing Lu, Shengsheng Lu, Kehuan Lu   +6 more
doaj   +1 more source

Combined refinements to somatic cell nuclear transfer methods improve porcine embryo development. [PDF]

J Reprod Dev, 2020
Dang-Nguyen TQ, Wells D, Haraguchi S, Men NT, Nguyen HT, Noguchi J, Kaneko H, Kikuchi K.   +7 more
europepmc   +3 more sources