Results 111 to 120 of about 101,802 (324)

Directed Evolution Improves the Catalytic Efficiency of APEX2‐Mediated Proximity‐Dependent RNA Labeling

Advanced Science, EarlyView.
ABSTRACT Engineered ascorbate peroxidase APEX2 has been widely used for spatially restricted profiling of subcellular biomolecules, but its catalytic efficiency toward newly developed probes such as biotin‐aniline (Btn‐An) remains suboptimal. To overcome this limitation, we performed yeast surface display‐based directed evolution to enhance APEX2 ...
Gang Wang, Yi Li, Peiyuan Meng, Peng Zou
wiley   +1 more source

Study Protocol: Comparison of Clinical Pregnancy Outcomes of Intracytoplasmic Sperm Injection (ICSI) Using Testicular and Ejaculated Spermatozoa in Male Patients with High DNA Fragmentation

Journal of Pharmacy and Bioallied Sciences
Background: This study aims to compare the clinical pregnancy outcome of intracytoplasmic sperm injection (ICSI) using testicular and ejaculated spermatozoa in male patients with high deoxyribonucleic acid (DNA) fragmentation.
Juhi Barai, Akash More, Namrata Choudhary   +2 more
doaj   +1 more source

TUNEL assay—Standardized method for testing sperm DNA fragmentation

Andrologia, 2020
Sperm DNA integrity is important for normal functions such as fertilization, implantation, pregnancy and fetal development. Sperm DNA fragmentation (SDF) is more common in infertile men and may be responsible for poor reproductive function.
R. Sharma, C. Iovine, A. Agarwal, R. Henkel   +3 more
semanticscholar   +1 more source

REPRODUCTION AND SPERM DNA FRAGMENTATION

Bulletin of Problems Biology and Medicine, 2019
The aim of this work was to search and analyze literature data about sperm DNA fragmentation and its role in reproduction. There are reports that men of couples with infertility have more sperm with DNA damage. If the number of sperm DNA damage exceeds 25-30% the probability of in vivo fertilization and intrauterine insemination is estimated to be ...
Kaleinikova O. M., Sribna V. O., Vinogradova-Anyk - O. O., Voznesenskaya T. Y., Blashkiv T. V.   +4 more
openaire   +2 more sources

The Landscape and Regulation of Histone Crotonylation in Mammalian Gametes and Early Embryos

Advanced Science, EarlyView.
Histone crotonylation undergoes a genome‐wide transition from broad domains to canonical narrow peaks during minor zygotic genome activation (ZGA). This remodeling is required for proper major ZGA and blastocyst formation. Disruption of this transition by transcriptional inhibition, metabolic perturbation, or HDAC1 dysfunction impairs embryonic ...
Shenli Yuan, Yelian Yan, Yalin Liang, Kanghua Zhong, Zhican Fu, Xiaobo Wang, Chao Liu, Tao Huang, Keliang Wu   +8 more
wiley   +1 more source

Can Altered Expression of HSPA2 in Varicocele Patients Lead to Abnormal Spermatogenesis? [PDF]

International Journal of Fertility and Sterility, 2010
Background Heat shock protein A2 (HSPA2) is correlated with sperm maturity and function. Therefore, dysfunctional expression of this gene results in abnormal spermatogenesis.
Mohammad Hossein Nasr Esfahani, Homayoun Abbasi, Zahra Mirhosseini, Nazem Ghasemi, Shahnaz Razavi, Marziyeh Tavalaee, Somayeh Tanhaei, Mohammad Reza Deemeh, Kamran Ghaedi, Farzaneh Zamansoltani, Farzad Rajaei   +10 more
doaj   +1 more source

The relationship between seminal leukocytes, oxidative status in the ejaculate, and apoptotic markers in human spermatozoa [PDF]

, 2013
The aim of this study was to investigate the relationship between seminal leukocytes, reactive oxygen species (ROS) production in the ejaculate, and markers of apoptosis in human spermatozoa.
Aitken R.J., Aitken R.J., Alvarez J.G., Aziz N., Barroso G., Cleyson Mupfiga, David Fisher, De Iuliis G.N., Depuydt C., Eggert-Kruse W., Eley A., Evenson D., Gallon F., Gorczyca W., Grunewald S., Henkel R., Henkel R., Henkel R., Henkel R., Henkel R., Kasai T., Koppers A.J., Lopes S., MacLeod J., Marchetti C., Martin G., Myhre O., Nicholson D.W., Ochsendorf F.R., Oehninger S., Paasch U., Pasqualotto F.F., Print C.G., Quinn P., Raha S., Ralf Henkel, Ricci G., Sakkas D., Sakkas D., Schaeffer A.J., Shekarriz M., Sun J.G., Thinus Kruger, Tremellen K., Villegas J., Wang X., Weidner W., World Health Organization, Zorn B.   +48 more
core   +1 more source

The effect of sperm DNA fragmentation index on assisted reproductive technology outcomes and its relationship with semen parameters and lifestyle.

Translational Andrology and Urology, 2019
Background The sperm DNA fragmentation index (DFI) is widely regarded as a key measure for assessing male fertility, but the predictive value of the DFI for outcomes of assisted reproductive technology (ART) remains under debate. In this study, we used a
Hong-yi Yang, Gang Li, Haixia Jin, Yihong Guo, Ying-pu Sun   +4 more
semanticscholar   +1 more source

Sperm DNA fragmentation: laboratory and clinical aspects [PDF]

Translational Andrology and Urology, 2017
The commentary by Drs. Khalili and Agha-Rahimi on the sperm DNA fragmentation (SDF) clinical guideline article by Agarwal et al . (1) recognized the presence of a negative association between high seminal SDF levels and fertility outcome specifically with assisted reproductive techniques (ART).
Majzoub, Ahmad, Agarwal, Ashok, Esteves, Sandro C.   +2 more
openaire   +2 more sources

Cold Gas Plasma Induces Platelet Activation and Hemostasis in Native and Anticoagulated Human Blood

Advanced NanoBiomed Research, EarlyView.
Cold physical gas plasma induces platelet activation and hemostatic responses in native and anticoagulated human blood. Treatment efficacy depends on plasma parameters, including gas composition, distance, and exposure mode, which govern reactive species delivery and energy transfer.
Sander Bekeschus, Broder Poschkamp, Lea Miebach, Linus Hübner, Julia van der Linde   +4 more
wiley   +1 more source