Results 21 to 30 of about 16,857 (217)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Acridine Orange and Flow Cytometry: Which Is Better to Measure the Effect of Varicocele on Sperm DNA Integrity?

Advances in Urology, 2015
We evaluated the effect of varicocelectomy on semen parameters and levels of sperm DNA damage in infertile men. A total of 75 infertile men with varicocele and 40 fertile men (controls) were included in this study.
Essam-Elden M. Mohammed, Eman Mosad, Asmaa M. Zahran, Diaa A. Hameed, Emad A. Taha, Mohamed A. Mohamed   +5 more
doaj   +1 more source

Sperm chromatin dispersion test before sperm preparation is predictive of clinical pregnancy in cases of unexplained infertility treated with intra-uterine insemination and induction with clomiphene citrate

Frontiers in Medicine, 2016
Background/Aims: A large proportion of men with normal sperm results as analyzed using conventional techniques have fragmented DNA in their spermatozoa.
FRANK W.R.C. VANDEKERCKHOVE, ILSE DE CROO, JAN GERRIS, ETIENNE VANDEN ABBEEL, PETRA DE SUTTER   +4 more
doaj   +1 more source

RETRACTED: Structural disorders of the sperm chromatin. Pathophysiological aspects. Clinical relevance

Вестник урологии, 2021
RETRACTED ARTICLEThe review provides an analysis of domestic and foreign sources devoted to the study of the sperm chromatin structure. The pathogenetic pathways of the sperm DNA fragmentation formation are described.
M. N. Korshunov, E. S. Korshunova, P. S. Kyzlasov, D. M. Korshunov, S. P. Darenkov   +4 more
doaj   +1 more source

PDIA6–SCD1 Axis Rewires Lipid Metabolism to Drive Gastric Cancer Progression

Advanced Science, EarlyView.
Protein disulfide isomerase A6 (PDIA6) is identified as an oncogenic driver in gastric cancer. PDIA6 directly binds and stabilizes SCD1 by limiting its ubiquitin–proteasome‐mediated degradation, thereby sustaining monounsaturated fatty acid (MUFA)‐enriched lipid homeostasis and lipid metabolic reprogramming.
Zhen Tian, Yifan Cheng, Jiajie Zhou, Ruiqi Li, Shuai Zhao, Ben Li, Zijie Xu, Mengli Zi, Yayan Fu, Chenkai Zhang, Qiannan Sun, Shantanu Baral, Sen Wang, Daorong Wang   +13 more
wiley   +1 more source

Sperm DNA fragmentation and oxidation are independent of malondialdheyde

Reproductive Biology and Endocrinology, 2011
Background There is clinical evidence to show that sperm DNA damage could be a marker of sperm quality and extensive data exist on the relationship between DNA damage and male fertility status.
Gargouri Jalel, Ben Hamida Afifa, Abdallah Fatma, Elleuch Henda, Chakroun Nozha, Zribi Nassira, Fakhfakh Faiza, Keskes Leila   +7 more
doaj   +1 more source

Stem Cell Differentiation Disperses Transcriptional Clusters via a Conserved Surface‐Condensate Trajectory

Advanced Science, EarlyView.
Stem cell differentiation follows a conserved surface condensate trajectory: H3K27ac super enhancers nucleate large RNA polymerase II clusters that grow and unfold before transcriptional activity disperses them. This work reveals how biophysical forces at enhancer surfaces dynamically build and dismantle stem cell transcription hubs, reshaping cell ...
Tim Klingberg, Irina Wachter, Agnieszka Pancholi, Matthias Akyel, Yomna Gohar, Priya Kumar, Ana Miguel Fernandes, Yuzhi Bao, Alica Schmidt‐Heydt, Marcel Piepers, Alicia Günthel, Marcel Sobucki, Elisa Kämmer, Süheyla Eroğlu‐Kayıkçı, Stephan Allgeier, Sylvia Erhardt, Vasily Zaburdaev, Carmelo Ferrai, Lennart Hilbert   +18 more
wiley   +1 more source

NAD+ Metabolism Licenses Zygotic Genome Activation via PARP7‐Mediated ADP‐Ribosylation of UHRF1 in Mouse Early Embryos

Advanced Science, EarlyView.
This study uncovers a metabolic‐epigenetic axis licensing zygotic genome activation (ZGA) in mouse embryos. A developmental decline in NAD+ levels activates PARP7, which mono‐ADP‐ribosylates and stabilizes UHRF1. This modification promotes the establishment of permissive histone acetylation marks, thereby facilitating timely ZGA.
Guangyi Cao, Ziqi Zhang, Anqi Chen, Yanbo Liu, Yuling Lin, Lina Yu, Ruixin Shi, Aolei Guo, Yan Mao, Ganggui Lou, Chaojun Li, Luhong Wen, Guijun Yan, Haixiang Sun   +13 more
wiley   +1 more source

Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal

Journal of Family and Reproductive Health
Objective: To determine the sperm DNA fragmentation in the semen of suspected sub-fertile men of Nepal and find its association with bacteriospermia demographic and lifestyle factors. Materials and methods: A cross-sectional study was conducted with the
Anima Shrestha, Dev Raj Joshi, Dijan Vaidya, Sanu Maiya Shrestha, Anjana Singh   +4 more
doaj   +1 more source

Effect of microsurgical varicocelectomy on sperm DNA fragmentation in infertile men

Middle East Fertility Society Journal, 2011
Objectives: To examine the effect of microsurgical varicocelectomy on the sperm DNA damage in infertile men. Setting: Private clinical practice. Design: Prospective study.
Sherif Ghazi, Howida Abdelfattah
doaj   +1 more source