Results 41 to 50 of about 16,246 (249)

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

A Surface‐engineered Microfluidic Device for Antibody‐Mediated Negative Selection of High‐Quality Sperm for Assisted Reproduction

Advanced Functional Materials, EarlyView.
This study reports a microfluidic device with a functionalized surface utilizing a polyoxazoline coating and covalently immobilized gold nanoparticles and anti‐phosphatidylserine antibody. The device efficiently eliminates pre‐apoptotic and apoptotic spermatozoa and yields sperm with substantially improved quality and low DNA damage, offering a simple ...
Soraya Rasi Ghaemi, David J. Sharkey, Nicole O. McPherson, Markos Negash Alemie, Krasimir Vasilev, Sarah A. Robertson   +5 more
wiley   +1 more source

Effect of microsurgical varicocelectomy on sperm DNA fragmentation in infertile men

Middle East Fertility Society Journal, 2011
Objectives: To examine the effect of microsurgical varicocelectomy on the sperm DNA damage in infertile men. Setting: Private clinical practice. Design: Prospective study.
Sherif Ghazi, Howida Abdelfattah
doaj   +1 more source

Amphibian sperm DNA fragmentation [PDF]

, 2016
There are extremely limited studies that have investigated DNA fragmentation in amphibians and even less information exists pertaining to DNA fragmentation in the spermatozoa. A DNA fragmentation assay applied and validated for amphibian spermatozoa would not only provide the first evidence of this phenomenon in a new major taxon, but also prove a ...
openaire   +2 more sources

Reactive oxygen species and sperm DNA fragmentation [PDF]

Translational Andrology and Urology, 2017
Dr. Dada demonstrated her extensive knowledge about sperm DNA fragmentation (SDF) in her elegantly written commentary with a comprehensive discussion on issues such as genetic health of offspring, value of SDF testing in lifestyle factors, modification of SDF by oocyte, role of sperm preparation, concept of reductive stress, methodology of SDF testing,
Agarwal, Ashok, Cho, Chak-Lam, Esteves, Sandro C., Majzoub, Ahmad   +3 more
openaire   +2 more sources

Paternal Circadian Disruption Impairs Offspring Cognition via Sperm microRNAs

Advanced Science, EarlyView.
Paternal circadian disruption remodels the sperm small RNA payload, elevating miR‐92a‐3p/miR‐25‐3p levels and perturbing early embryonic gene regulatory programs. Microinjection experiments and single‐embryo transcriptomics reveal sex‐specific developmental vulnerabilities, ultimately impairing offspring hippocampal synaptic plasticity and cognition ...
Kexin Zou, Sisi Luo, Binliang Tang, Zhiqiang Liu, Yuchuan Zhou, Yicong Meng, Zhengmu Wu, Zheng Sun, Weihui Shi, Jianzhong Sheng, Chuanjin Yu, Zhenhua Li, Siyi Wei, Mo Zhang, Yujie Luo, Jing Yan, Xueyun Qin, Jiahang Mo, Chengliang Zhou, Jinsong Li, Hefeng Huang, Yifu Ding, Guolian Ding   +22 more
wiley   +1 more source

Influence of Bacteriospermia, Host and Lifestyle Factors on Sperm DNA Integrity: A Cross-Sectional Study Based on a Fertility Center of Nepal

Journal of Family and Reproductive Health
Objective: To determine the sperm DNA fragmentation in the semen of suspected sub-fertile men of Nepal and find its association with bacteriospermia demographic and lifestyle factors. Materials and methods: A cross-sectional study was conducted with the
Anima Shrestha, Dev Raj Joshi, Dijan Vaidya, Sanu Maiya Shrestha, Anjana Singh   +4 more
doaj   +1 more source

Comparison of three sperm selection methods for ICSI-DGC, Cumulus column, and incubation with supernatant product of adipose tissue-derived adult stem cells: An experimental study

International Journal of Reproductive BioMedicine, 2021
Background: The examination of sperm parameters and sperm DNA integrity are necessary for male fertility expression. These parameters can be affected by method of sperm separation.
Zeynab Yazdanpanah, Mitra Heydari Nasrabadi, Zeynab Piravar   +2 more
doaj   +1 more source

Sperm swim-up techniques and DNA fragmentation [PDF]

Human Reproduction, 2001
Swim-up techniques for sperm separation may have detrimental effects on sperm DNA. We wished to determine whether the normal swim-up method with centrifugation used in our laboratory, which involves a centrifugation step, was harmful to sperm compared with swim-up without centrifugation.Semen samples were obtained from patients undergoing IVF or ...
E V, Younglai, D, Holt, P, Brown, A, Jurisicova, R F, Casper   +4 more
openaire   +2 more sources

Zinc Exposure Causes Disulfidptosis to Induce Miscarriage by Up‐Regulating GATA1/METTL1/SLC7A11 Axis

Advanced Science, EarlyView.
Zn exposure up‐regulates GATA1, promoting GATA1‐mediated METTL1 and SLC7A11 transcription. It also enhances METTL1‐mediated m7G modification on SLC7A11 mRNA, increasing SLC7A11 mRNA stability. Ultimately, Zn exposure up‐regulates SLC7A11 at both transcriptional and post‐transcriptional levels, causing disulfidptosis. Knockdown of murine Slc7a11, Gata1,
Wenxin Huang, Yi Sun, Yanxin Wang, Haijun Yan, Xiaoping Yue, Weidong Wu, Xueyu Chen, Jinfang Zhang, Yanbing Lin, Qiong Lei, Nan Ji, Shuaishuai Xing, Liqin Zeng, Qingzheng Kang, Depeng Zhao, Geng Guo, Huidong Zhang   +16 more
wiley   +1 more source