Results 61 to 70 of about 60,784 (278)

Controllable Dynamic Mechanical Cell Stimulation using Magnetically Actuated Artificial Cilia

Advanced Healthcare Materials, EarlyView.
This paper introduces a platform based on magnetic artificial cilia for providing controllable dynamic mechanical stimulation to single cells, suitable for investigating large cell populations and enabling live cell imaging. Proof‐of‐principle experiments show that cell morphology is strongly influenced by the artificial cilia, that cellular forces can
Roel Kooi, Tanveer Ul Islam, Oscar M.J.A. Stassen, Naomie Amsing, Jan de Boer, Jaap M.J. den Toonder   +5 more
wiley   +1 more source

Microcephaly-associated protein WDR62 shuttles from the Golgi apparatus to the spindle poles in human neural progenitors

eLife, 2023
WDR62 is a spindle pole-associated scaffold protein with pleiotropic functions. Recessive mutations in WDR62 cause structural brain abnormalities and account for the second most common cause of autosomal recessive primary microcephaly (MCPH), indicating ...
Claudia Dell'Amico, Marilyn M Angulo Salavarria, Yutaka Takeo, Ichiko Saotome, Maria Teresa Dell'Anno, Maura Galimberti, Enrica Pellegrino, Elena Cattaneo, Angeliki Louvi, Marco Onorati   +9 more
doaj   +1 more source

LSD1 is essential for oocyte meiotic progression by regulating CDC25B expression in mice [PDF]

, 2015
Mammalian oocytes are arrested at prophase I until puberty when hormonal signals induce the resumption of meiosis I and progression to meiosis II.
Chen, T, Kerenyi, MA, Kim, J, Lin, K, Lu, Y, Orkin, SH, Shen, J, Singh, AK, Takata, Y   +8 more
core   +1 more source

Spindle assembly defects leading to the formation of a monopolar mitotic apparatus [PDF]

Biology of the Cell, 2009
Mitotic spindle formation in animal cells involves microtubule nucleation from two centrosomes that are positioned at opposite sides of the nucleus. Microtubules are captured by the kinetochores and stabilized. In addition, microtubules can be nucleated independently of the centrosome and stabilized by a gradient of Ran—GTP, surrounding the mitotic ...
Vanessa, Tillement, Marie-Hélène, Remy, Brigitte, Raynaud-Messina, Laurent, Mazzolini, Laurence, Haren, Andreas, Merdes   +5 more
openaire   +2 more sources

High‐Throughput 3D Matrigel‐based Droplet Microfluidics for Single‐Cell Function‐to‐Omics Analysis of Cytotoxic Immune Cells in Solid Tumor Interactions

Advanced Materials Technologies, EarlyView.
A 3D Matrigel‐based droplet microfluidics platform that allows physiologically relevant analysis of single immune‐tumor interactions in solid tumors. By integrating real‐time functional imaging with fluorescence‐activated droplet sorting and RNA sequencing, the system links immune cytotoxic behavior to transcriptomic profiles, providing a scalable ...
Christina Sharkey, Harry Akligoh, Michael Finocchiaro, Abhishek Srihari Rampelli, Zongwei Wang, Aria F. Olumi, Tania Konry   +6 more
wiley   +1 more source

Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis [PDF]

, 2017
Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working at the resolution limit of the method, we describe a correlative light-SBF-SEM workflow to resolve microtubules of the mitotic spindle in ...
Beckett, AJ, Brettschneider, JA, Clarke, NI, Honnor, TR, Johansen, AM, Nixon, FM, Prior, IA, Royle, SJ, Starling, GP   +8 more
core   +2 more sources

Scalable Layer‐by‐Layer Electrospray‐Assisted Interfacial Polymerization: Enabling Large‐Area Polyamide Membrane Fabrication

Advanced Materials Technologies, EarlyView.
We report a multi‐jet, layer‐by‐layer electrospray‐assisted interfacial polymerization (LBL‐EAIP) approach for the scalable fabrication of polyamide reverse osmosis (RO) membranes. The method enables precise control of polymerization, uniform polyamide coverage, and tunable polyamide film thickness, while delivering consistently high filtration ...
Yue Feng, Feng Gao, Mert Can Hacifazlioglu, Jason Zhao, Jeffrey R. McCutcheon, Yuepeng Zhang   +5 more
wiley   +1 more source

Chromosome movement in lysed mitotic cells is inhibited by vanadate. [PDF]

, 1978
Mitotic PtK1 cells, lysed at anaphase into a carbowax 20 M Brij 58 solution, continue to move chromosomes toward the spindle poles and to move the spindle poles apart at 50% in vivo rates for 10 min.
CANDE, W. Zacheus, Wolniak, S
core   +1 more source

Mitotic spindle scaling during Xenopus development by kif2a and importin α. [PDF]

, 2012
Early development of many animals is characterized by rapid cleavages that dramatically decrease cell size, but how the mitotic spindle adapts to changing cell dimensions is not understood.
HEALD, Rebecca, Wilbur, Jeremy
core   +2 more sources

Large‐Scale and Cost‐Effective Fabrication of Ultra‐Thin, Biodegradable Microelectrode Arrays and Pressure Sensors Using Laser Micromachining

Advanced Materials Technologies, EarlyView.
A mask‐free and cost‐effective UV‐pico‐second laser‐based microfabrication method is proposed to fabricate large‐area biodegradable microelectrode arrays and pressure sensors. These devices demonstrate low impedance, good conformability, excellent biocompatibility, and rapid degradation, providing a new route for next‐generation transient electronics ...
Bhavani Prasad Yalagala, Asfand Tanwear, María Cerezo‐Sánchez, Hossein Parvizi, Morteza Amjadi, Hadi Heidari   +5 more
wiley   +1 more source