Results 11 to 20 of about 143,230 (264)
Dual-specificity splice sites function alternatively as 5′ and 3′ splice sites [PDF]
Proceedings of the National Academy of Sciences, 2007 As a result of large-scale sequencing projects and recent splicing-microarray studies, estimates of mammalian genes expressing multiple transcripts continue to increase. This expansion of transcript information makes it possible to better characterize alternative splicing events and gain insights into splicing mechanisms and regulation.
Chaolin, Zhang +3 more
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m6A modification of U6 snRNA modulates usage of two major classes of pre-mRNA 5’ splice site
eLife, 2022 Alternative splicing of messenger RNAs is associated with the evolution of developmentally complex eukaryotes. Splicing is mediated by the spliceosome, and docking of the pre-mRNA 5’ splice site into the spliceosome active site depends upon pairing with ...
Matthew T Parker +11 more
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ERISdb: A Database of Plant Splice Sites and Splicing Signals [PDF]
Plant and Cell Physiology, 2013 Splicing is one of the major contributors to observed spatiotemporal diversification of transcripts and proteins in metazoans. There are numerous factors that affect the process, but splice sites themselves along with the adjacent splicing signals are critical here.
Michał Wojciech, Szcześniak +4 more
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Splicing proofreading at 5′ splice sites by ATPase Prp28p [PDF]
Nucleic Acids Research, 2013 Fidelity and efficiency of pre-mRNA splicing are critical for generating functional mRNAs, but how such accuracy in 5' splice site (SS) selection is attained is not fully clear. Through a series of yeast genetic screens, we isolated alleles of prp28 that improve splicing of suboptimal 5'SS substrates, demonstrating that WT-Prp28p proofreads, and ...
Yang, Fei +7 more
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PLoS Genetics, 2022 Pre-mRNA splicing is an essential step of eukaryotic gene expression carried out by a series of dynamic macromolecular protein/RNA complexes, known collectively and individually as the spliceosome.
Jessie M N G L Suzuki +5 more
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iMapSplice: Alleviating reference bias through personalized RNA-seq alignment. [PDF]
PLoS ONE, 2018 Genomic variants in both coding and non-coding sequences can have functionally important and sometimes deleterious effects on exon splicing of gene transcripts.
Xinan Liu, James N MacLeod, Jinze Liu
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Conserved and divergent signals in 5' splice site sequences across fungi, metazoa and plants.
PLoS Computational Biology, 2023 In eukaryotic organisms the ensemble of 5' splice site sequences reflects the balance between natural nucleotide variability and minimal molecular constraints necessary to ensure splicing fidelity.
Maximiliano S Beckel +3 more
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Identifying RNA splicing factors using IFT genes in Chlamydomonas reinhardtii [PDF]
Open Biology, 2018 Intraflagellar transport moves proteins in and out of flagella/cilia and it is essential for the assembly of these organelles. Using whole-genome sequencing, we identified splice site mutations in two IFT genes, IFT81 (fla9) and IFT121 (ift121-2), which ...
Huawen Lin +3 more
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Frontiers in Plant Science, 2022 Cryptic splice sites in eukaryotic genome are generally dormant unless activated by mutation of authentic splice sites or related splicing factors. How cryptic splice sites are used remains unclear in plants.
Weihua Huang +7 more
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Mutations in the Caenorhabditis elegans U2AF large subunit UAF-1 alter the choice of a 3' splice site in vivo. [PDF]
PLoS Genetics, 2009 The removal of introns from eukaryotic RNA transcripts requires the activities of five multi-component ribonucleoprotein complexes and numerous associated proteins.
Long Ma, H Robert Horvitz
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