Results 41 to 50 of about 48,897 (224)

The spliceosome inhibitors isoginkgetin and pladienolide B induce ATF3-dependent cell death.

PLoS ONE, 2020
The spliceosome assembles on pre-mRNA in a stepwise manner through five successive pre-spliceosome complexes. The spliceosome functions to remove introns from pre-mRNAs to generate mature mRNAs that encode functional proteins.
Erin J Vanzyl, Hadil Sayed, Alex B Blackmore, Kayleigh R C Rick, Pasan Fernando, Bruce C McKay   +5 more
doaj   +1 more source

The 35S U5 snRNP is generated from the activated spliceosome during In vitro splicing [PDF]

, 2015
Primary gene transcripts of eukaryotes contain introns, which are removed during processing by splicing machinery. Biochemical studies In vitro have identified a specific pathway in which introns are recognised and spliced out. This occurs by progressive
Barbara Bardoni, CL Will, CL Will, DE Agafonov, EM Makarov, EM Makarov, Evgeny M. Makarov, J Deckert, JD Dignam, K Hartmuth, MC Wahl, N Behzadnia, Olga V. Makarova, OV Makarova, OV Makarova, P Fabrizio, S Bessonov, S Bessonov, SE Behrens   +18 more
core   +2 more sources

αCP binding to a cytosine-rich subset of polypyrimidine tracts drives a novel pathway of cassette exon splicing in the mammalian transcriptome. [PDF]

, 2016
Alternative splicing (AS) is a robust generator of mammalian transcriptome complexity. Splice site specification is controlled by interactions of cis-acting determinants on a transcript with specific RNA binding proteins.
Bahrami-Samani, Emad, Duncan-Lewis, Christopher, Ji, Xinjun, Liebhaber, Stephen A, Lin, Lan, Park, Juw Won, Pherribo, Gordon, Xing, Yi   +7 more
core   +1 more source

PRP4: a protein of the yeast U4/U6 small nuclear ribonucleoprotein particle [PDF]

, 1989
The Saccharomyces cerevisiae prp mutants (prp2 through prp11) are known to be defective in pre-mRNA splicing at nonpermissive temperatures. We have sequenced the PRP4 gene and shown that it encodes a 52-kilodalton protein.
Abelson, John N., Banroques, Josette
core   +1 more source

Interaction of yeast eIF4G with spliceosome components Implications in pre-mRNA processing events [PDF]

, 2009
International audienceAs evidenced from mammalian cells the eukaryotic translation initiation factor eIF4G has a putative role in nuclear RNA metabolism. Here we investigate whether this role is conserved in the yeast Saccharomyces cerevisiae.
Barrass, J. David, Beggs, Jean D., Fromont-Racine, Micheline, Jacquier, Alain, Kafasla, Panagiota, Lewis, Joe, Thompson, Elizabeth   +6 more
core   +2 more sources

Challenging the spliceosome machine [PDF]

Genome Biology, 2006
Abstract Background Using cDNA copies of transcripts and corresponding genomic sequences from the Berkeley Drosophila Genome Project, a set of 24,753 donor and acceptor splice sites were computed with a scanning algorithm that tested for single nucleotide insertion, deletion and substitution ...
Weir, Michael, Eaton, Matthew, Rice, Michael   +2 more
openaire   +2 more sources

evolution, structure and function of metazoan splicing factor PRPF39 [PDF]

, 2019
In the yeast U1 snRNP the Prp39/Prp42 heterodimer is essential for early steps of spliceosome assembly. In metazoans no Prp42 ortholog exists, raising the question how the heterodimer is functionally substituted.
Bortoli, Francesca De, Heyd, Florian, Kotte, Ana, Loll, Bernhard, Neumann, Alexander, Schüler, Thomas, Timmermann, Bernd, Wahl, Markus C.   +7 more
core   +1 more source

Spliceosome Mutations in Uveal Melanoma [PDF]

International Journal of Molecular Sciences, 2020
Uveal melanoma (UM) is the most common primary intraocular malignancy of the eye. It has a high metastatic potential and mainly spreads to the liver. Genetics play a vital role in tumor classification and prognostication of UM metastatic disease. One of the driver genes mutated in metastasized UM is subunit 1 of splicing factor 3b (SF3B1), a component ...
Josephine Q.N. Nguyen, Wojtek Drabarek, Serdar Yavuzyigitoglu, Eva Medico Salsench, Robert M. Verdijk, Nicole C. Naus, Annelies de Klein, Emine Kiliç, Erwin Brosens   +8 more
openaire   +2 more sources

Molecular basis for the activation of human spliceosome

Nature Communications
The spliceosome executes pre-mRNA splicing through four sequential stages: assembly, activation, catalysis, and disassembly. Activation of the spliceosome, namely remodeling of the pre-catalytic spliceosome (B complex) into the activated spliceosome ...
Xiechao Zhan, Yichen Lu, Yigong Shi
doaj   +1 more source

Flow cytometric analysis identifies changes in S and M phases as novel cell cycle alterations induced by the splicing inhibitor isoginkgetin.

PLoS ONE, 2018
The spliceosome is a large ribonucleoprotein complex that catalyzes the removal of introns from RNA polymerase II-transcribed RNAs. Spliceosome assembly occurs in a stepwise manner through specific intermediates referred to as pre-spliceosome complexes E,
Erin J Vanzyl, Kayleigh R C Rick, Alex B Blackmore, Erin M MacFarlane, Bruce C McKay   +4 more
doaj   +1 more source