Results 91 to 100 of about 17,120 (319)

Single‐Cell RNA Sequencing Delineates Renal Anti‐Fibrotic Mechanisms Mediated by TRPC6 Inhibition

Advanced Science, EarlyView.
Single‐cell transcriptomics reveals how TRPC6 inhibition alters renal cell composition and gene expression in CKD. The study uncovers a novel endothelial subpopulation (ECRIN), highlights key inflammatory and fibrotic pathways, and identifies a Prnp‐driven network linked to fibrosis resolution, offering mechanistic insight into TRPC6 as a potential ...
Yao Xu, Zhihuang Zheng, Marleen Silke Oswald, Guozhe Cheng, Jun Liu, Qidi Zhai, Ute Kruegel, Michael Schaefer, Holger Gerhardt, Nicole Endlich, Maik Gollasch, Stefan Simm, Dmitry Tsvetkov   +12 more
wiley   +1 more source

A Prion‐Like Domain in EBV EBNA1 Promotes Phase Separation and Enables SRRM1 Splicing

Advanced Science, EarlyView.
This study discoveries that EBV EBNA1 behaves as a prion‐like protein, verified using cell‐based assays and the Saccharomyces cerevisiae Sup35p prion identification system. The prion‐like domain of EBNA1 drives liquid–liquid phase separation. EBNA1 interacts with the splicing factor SRSF1 to regulate the expression of the SRRM1 splicing isoforms ...
Xiaoyue Zhang, Zhengshuo Li, Run Zheng, Xiang Zheng, Jia Wang, Can Liu, Yangge Wu, Yuqing Wen, Chunlin Ou, Songqing Fan, Chenxiao Xu, Junrui Tian, Qun Yan, Hao Nan, Xiaodong Xu, Hui Wang, Qiu Peng, Jian Ma   +17 more
wiley   +1 more source

The biological function and clinical significance of SF3B1 mutations in cancer

Biomarker Research, 2020
Spliceosome mutations have become the most interesting mutations detected in human cancer in recent years. The spliceosome, a large, dynamic multimegadalton small nuclear ribonucleoprotein composed of small nuclear RNAs associated with proteins, is ...
Zhixia Zhou, Qi Gong, Yin Wang, Mengkun Li, Lu Wang, Hongfei Ding, Peifeng Li   +6 more
doaj   +1 more source

Saccharomyces cerevisiae NineTeen Complex (NTC)-associated Factor Bud31/Ycr063w Assembles on Precatalytic Spliceosomes and Improves First and Second Step Pre-mRNA Splicing Efficiency*

Journal of Biological Chemistry, 2012
Background: Some yeast splicing factors, e.g. Bud31 in Cef1p subcomplex, are nonessential for cell viability. Results: Bud31 occurs in precatalytic (B) and catalytic spliceosomes and stabilizes protein interactions with the pre-mRNA; its absence affects ...
Debjani Saha, P. Khandelia, R. O’Keefe, U. Vijayraghavan   +3 more
semanticscholar   +1 more source

RNA Nuclear Export: From Neurological Disorders to Cancer [PDF]

, 2017
The presence of a nuclear envelope, also known as nuclear membrane, defines the structural framework of all eukaryotic cells by separating the nucleus, which contains the genetic material, from the cytoplasm where the synthesis of proteins takes place ...
Hautbergue, G.M.
core   +1 more source

Engineered Cas12j‐8 is a Versatile Platform for Multiplexed Genome Modulation in Mammalian Cells

Advanced Science, EarlyView.
Engineered through structure‐guided protein engineering, enCas12j‐8 significantly enhances the editing efficiency of compact Cas12j‐8 while maintaining high specificity. It enables efficient multiplexed genome editing and base editing using a single crRNA array, demonstrating broad applicability and therapeutic potential in genome engineering ...
Ru Meng, Jiayao Li, Wuke Wang, Dong Liang, Zhanwei Li, Cong Mao, Qingyang Li, Yu Zhang, Hao Chen, Jin Tang, Ping Hu, Qi Niu, Xingxu Huang, Bin Shen, Jun Zhang   +14 more
wiley   +1 more source

Expression of PDLIM5 Spliceosomes and Regulatory Functions on Myogenesis in Pigs

Cells
Meat yield, determined by muscle growth and development, is an important economic trait for the swine industry and a focus of research in animal genetics and breeding. PDZ and LIM domain 5 (PDLIM5) are cytoskeleton-related proteins that play key roles in
Yu Fu, Shixin Li, Jingru Nie, Dawei Yan, Bo Zhang, Xin Hao, Hao Zhang   +6 more
doaj   +1 more source

Defective minor spliceosome mRNA processing results in isolated familial growth hormone deficiency [PDF]

, 2014
The molecular basis of a significant number of cases of isolated growth hormone deficiency remains unknown. We describe three sisters affected with severe isolated growth hormone deficiency and pituitary hypoplasia caused by biallelic mutations in the ...
Ali Oghabian, Armand Gutiérrez‐Arumí, Bhupendra Verma, Gabriel Á Martos‐Moreno, Ivon Cuscó, Jesús Argente, Julie A Chowen, Luis A Pérez‐Jurado, Mikko J Frilander, Pfäffle R, Pérez Jurado LA, Raquel Flores   +11 more
core   +2 more sources

Splicing Shift of RAC1 Accelerates Tumorigenesis and Defines a Potent Therapeutic Target in Lung Cancer

Advanced Science, EarlyView.
Dysregulated RNA splicing is an underappreciated molecular feature of cancer. By integrating murine and cellular models with patient specimens of lung adenocarcinoma, this study demonstrates that RAC1B, rather than RAC1A isoform, promotes tumorigenesis and presents a potent therapeutic target for lung cancer. Mechanistically, RAC1B preferentially binds
Yueren Yan, Ning Wang, Bowen Xing, Min Yang, Jun Shang, Yufang Bao, Lixing Xiao, Ningxia Zhang, Yunpeng Ren, Chunnan Liu, Yuting Chen, Han Han, Yunjian Pan, Lei Lv, Wei‐Xing Zong, Hongbin Ji, Changyou Zhan, Zefeng Wang, Haiquan Chen, Yongbo Wang   +19 more
wiley   +1 more source

Cloning and molecular characterization of Trypanosoma cruzi U2, U4, U5, and U6 small nuclear RNAs

Memorias do Instituto Oswaldo Cruz, 2007
Small nuclear RNAs (snRNAs) are important factors in the functioning of eukaryotic cells that form several small complexes with proteins; these ribonucleoprotein particles (U snRNPs) have an essential role in the pre-mRNA processing, particularly in ...
DL Ambrósio, MTA Silva, RMB Cicarelli
doaj   +1 more source