Results 101 to 110 of about 6,327 (242)

Characterisation of the complexes released into the supernatant from immobilised activated spliceosomes during splicing in the presence or absence of ATP by glycerol gradient centrifugation.

, 2015
(A) The RNA extracted from each gradient fraction was fractionated by denaturing PAGE and the 32P-containing species were detected by autoradiography. S-values were determined by comparison with the reference gradients containing 30S and 50S ribosomal ...
Olga V. Makarova (259232), Evgeny M. Makarov (259236)   +1 more
core   +1 more source

The RES complex is required for efficient transformation of the precatalytic B spliceosome into an activated B^act complex [PDF]

, 2017
The precise function of the trimeric retention and splicing (RES) complex in pre-mRNA splicing remains unclear. Here we dissected the role of RES during the assembly and activation of yeast spliceosomes.
Henning Urlaub, Penghui Bao, Reinhard Lührmann, Will, Cindy L., Urlaub, Henning, Urlaub, H., Boon, K., Bao, P., Cindy L. Will, Kum-Loong Boon, Boon, Kum-Loong, Bao, Penghui, Will, C., Lührmann, R., Lührmann, Reinhard   +14 more
core   +1 more source

A multi-factor trafficking site on the spliceosome remodeling enzyme BRR2 recruits C9ORF78 to regulate alternative splicing

, 2022
The intrinsically unstructured C9ORF78 protein was detected in spliceosomes but its role in splicing is presently unclear. We find that C9ORF78 tightly interacts with the spliceosome remodeling factor, BRR2, in vitro.
Preußner, M., Preu ner, M., Wahl, M., Heyd, Florian, Ilik, I., Hilal, Tarek, Aktaş, Tuğçe, Bergfort, A., Ilik, I.A., Hilal, T., Weber, Gert, Bergfort, Alexandra, Weber, G., Preußner, Marco, Wahl, Markus C., Kuropka, Benno, Wahl, M.C., Aktas, T., Freund, Christian, Kuropka, B., Freund, C., Heyd, F., Ilik, İbrahim Avşar   +22 more
core   +1 more source

MTAP Deficiency as a Metabolic Vulnerability in Cancer: Implications for Synthetic Lethal Therapy

Cancer Science, EarlyView.
MTAP deletion creates a therapeutically actionable metabolic vulnerability through MTA accumulation and PRMT5 dependency. This review summarizes the biochemical basis of MTAP‐directed synthetic lethality, emerging PRMT5/MAT2A inhibitors, clinicogenomic features of MTAP‐deleted tumors, and future strategies for precision oncology.
Hiroaki Ikushima, Hidenori Kage
wiley   +1 more source

Targeting the microbiota‐miRNA‐protease axis: A new therapeutic avenue in melanoma

The FEBS Journal, EarlyView.
Modulation of extracellular matrix (ECM) turnover is central to melanoma progression and metastasis, driven largely by ECM proteases. This review highlights the epigenetic regulation of ECM proteases by microRNAs and their roles in melanoma growth, invasion, and immune modulation.
Elias N. Katsoulieris, Paraskevi Ioannou, Nikolaos A. Afratis   +2 more
wiley   +1 more source

miRNA Enriched in Human Neuroblast Nuclei Bind the MAZ Transcription Factor and Their Precursors Contain the MAZ Consensus Motif

Frontiers in Molecular Neuroscience, 2017
While the cytoplasmic function of microRNA (miRNA) as post-transcriptional regulators of mRNA has been the subject of significant research effort, their activity in the nucleus is less well characterized.
Belinda J. Goldie, Belinda J. Goldie, Belinda J. Goldie, Chantel Fitzsimmons, Chantel Fitzsimmons, Judith Weidenhofer, Joshua R. Atkins, Joshua R. Atkins, Dan O. Wang, Dan O. Wang, Murray J. Cairns, Murray J. Cairns   +11 more
doaj   +1 more source

Quantitative analysis of DNA‐GATA1 binding alterations linked to hematopoietic disorders

The FEBS Journal, EarlyView.
Native holdup allows the quantitative determination of affinities between full‐length transcription factors and DNA. Mutations in either the protein or the DNA can modulate binding strength, which can be precisely quantified using this approach. Applied to GATA1, it revealed mutations that alter DNA binding.
Boglarka Zambo, Bastien Morlet, Luc Negroni, Orsolya Mózner, Balázs Sarkadi, Gilles Travé, Gergo Gogl   +6 more
wiley   +1 more source

Disruption of murine mp29/Syf2/Ntc31 gene results in embryonic lethality with aberrant checkpoint response.

PLoS ONE, 2012
Human p29 is a putative component of spliceosomes, but its role in pre-mRNA is elusive. By siRNA knockdown and stable overexpression, we demonstrated that human p29 is involved in DNA damage response and Fanconi anemia pathway in cultured cells.
Chia-Hsin Chen, Po-Chen Chu, Liekyeow Lee, Huang-Wei Lien, Tse-Ling Lin, Chi-Chen Fan, Peter Chi, Chang-Jen Huang, Mau-Sun Chang   +8 more
doaj   +1 more source

Post‐transcriptional regulatory networks: The dynamic interplay of RNA‐binding proteins

The FEBS Journal, EarlyView.
Post‐transcriptional gene expression regulation is controlled by RNA‐binding proteins (RBPs), which influence many processes including splicing, localization, translation, and decay. Rather than acting alone, RBPs bind RNAs combinatorially, forming complex, context‐dependent regulatory networks.
Lena A. Street, Marko Jovanovic, Eugenio F. Fornasiero   +2 more
wiley   +1 more source

Immunoprecipitation of spliceosomal RNAs by antisera to galectin-1 and galectin-3

, 2006
We have shown that galectin-1 and galectin-3 are functionally redundant splicing factors. Now we provide evidence that both galectins are directly associated with spliceosomes by analyzing RNAs and proteins of complexes immunoprecipitated by galectin ...
Weizhong Wang, Jung W. Park, John L. Wang, Ronald J. Patterson   +3 more
core   +1 more source