Results 71 to 80 of about 17,120 (319)

Mechanism for the initiation of spliceosome disassembly. [PDF]

Nature
Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome1. Recent studies have shed light on spliceosome assembly and remodelling for catalysis2-6, but the mechanism of disassembly remains unclear.
Vorländer MK, Rothe P, Kleifeld J, Cormack ED, Veleti L, Riabov-Bassat D, Fin L, Phillips AW, Cochella L, Plaschka C.   +9 more
europepmc   +3 more sources

Structure of a pre-catalytic spliceosome [PDF]

Nature, 2017
Intron removal requires assembly of the spliceosome on precursor mRNA (pre-mRNA) and extensive remodelling to form the spliceosome's catalytic centre. Here we report the cryo-electron microscopy structure of the yeast Saccharomyces cerevisiae pre-catalytic B complex spliceosome at near-atomic resolution.
Plaschka, Clemens, Lin, Pei-Chun, Nagai, Kiyoshi   +2 more
openaire   +3 more sources

Multidimensional OMICs reveal ARID1A orchestrated control of DNA damage, splicing, and cell cycle in normal‐like and malignant urothelial cells

Molecular Oncology, EarlyView.
Loss of the frequently mutated chromatin remodeler ARID1A, a subunit of the SWI/SNF cBAF complex, results in less open chromatin, alternative splicing, and the failure to stop cells from progressing through the cell cycle after DNA damage in bladder (cancer) cells. Created in BioRender. Epigenetic regulators, such as the SWI/SNF complex, with important
Rebecca M. Schlösser, Florian Krumbach, Eyleen Corrales, Geoffroy Andrieux, Christian Preisinger, Franziska Liss, Alexandra Golzmann, Melanie Boerries, Kerstin Becker, Ruth Knüchel, Stefan Garczyk, Bernhard Lüscher   +11 more
wiley   +1 more source

TDRD6 mediates early steps of spliceosome maturation in primary spermatocytes. [PDF]

PLoS Genetics, 2017
Tudor containing protein 6 (TDRD6) is a male germ line-specific protein essential for chromatoid body (ChB) structure, elongated spermatid development and male fertility.
Müge Akpınar, Mathias Lesche, Grigorios Fanourgakis, Jun Fu, Konstantinos Anastassiadis, Andreas Dahl, Rolf Jessberger   +6 more
doaj   +1 more source

Prognostic value of PNN in prostate cancer and its correlation with therapeutic significance

Frontiers in Genetics, 2022
Prostate cancer (PCa) is the most common malignancy. New biomarkers are in demand to facilitate the management. The role of the pinin protein (encoded by PNN gene) in PCa has not been thoroughly explored yet.
Ruisong Wang, Ruisong Wang, Ruisong Wang, Ziyi Qin, Huiling Luo, Meisen Pan, Meisen Pan, Mingyao Liu, Mingyao Liu, Pinhong Yang, Pinhong Yang, Pinhong Yang, Tieliu Shi, Tieliu Shi   +13 more
doaj   +1 more source

Structures of the human pre-catalytic spliceosome and its precursor spliceosome [PDF]

Cell Research, 2018
The pre-catalytic spliceosome (B complex) is preceded by its precursor spliceosome (pre-B complex) and followed by the activated spliceosome (Bact complex). The pre-B-to-B and B-to-Bact transitions are driven by the ATPase/helicases Prp28 and Brr2, respectively. In this study, we report the cryo-electron microscopy structures of the human pre-B complex
Xiechao Zhan, Chuangye Yan, Xiaofeng Zhang, Jianlin Lei, Yigong Shi   +4 more
openaire   +2 more sources

Tonic signaling of the B‐cell antigen‐specific receptor is a common functional hallmark in chronic lymphocytic leukemia cell phosphoproteomes at early disease stages

Molecular Oncology, EarlyView.
B‐cell chronic lymphocytic leukemia (B‐CLL) and monoclonal B‐cell lymphocytosis (MBL) show altered proteomes and phosphoproteomes, analyzed using mass spectrometry, protein microarrays, and western blotting. Identifying 2970 proteins and 316 phosphoproteins, including 55 novel phosphopeptides, we reveal BCR and NF‐kβ/STAT3 signaling in disease ...
Paula Díez, Pablo Juanes‐Velasco, Marina L. García‐Vaquero, Conrad Droste, Alicia Landeira‐Viñuela, Miguel Alcoceba, Helena Fidalgo‐Gómez, Sara Misiego‐Herrero, Almudena Navarro‐Bailón, Mónica Baile, José M. Bastida, Jose Manuel Sanchez‐Santos, Rafael Góngora, Julia Almeida, Marcos Gonzalez‐Diaz, Alberto Orfao, Javier De Las Rivas, Manuel Fuentes   +17 more
wiley   +1 more source

The role of nuclear organization in trans-splicing based expression of heat shock protein 90 in Giardia lamblia.

PLoS Neglected Tropical Diseases, 2021
Hsp90 gene of G. lamblia has a split nature comprising two ORFs separated by 777 kb on chromosome 5. The ORFs of the split gene on chromosome 5 undergo transcription to generate independent pre-mRNAs that join by a unique trans-splicing reaction that ...
Vinithra Iyer, Sheetal Tushir, Shreekant Verma, Sudeshna Majumdar, Srimonta Gayen, Rakesh Mishra, Utpal Tatu   +6 more
doaj   +1 more source

Rearrangements within human spliceosomes captured after exon ligation.

RNA: A publication of the RNA Society, 2013
In spliceosomes, dynamic RNA/RNA and RNA/protein interactions position the pre-mRNA substrate for the two chemical steps of splicing. Not all of these interactions have been characterized, in part because it has not been possible to arrest the complex at
J. Ilagan, R. Chalkley, A. Burlingame, M. Jurica   +3 more
semanticscholar   +1 more source

Spliceosome assembly in yeast. [PDF]

Genes & Development, 1987
Precursors to mRNA become substrates for splicing by being assembled into a complex multisubunit structure, the spliceosome. To study the assembly of the yeast spliceosome, intermediate complexes were separated by electrophoresis on nondenaturing polyacrylamide gels. Four splicing-dependent complexes, A1, A2-1, A2-2, and B, were observed.
Soo-Chen Cheng, John Abelson
openaire   +3 more sources