Results 161 to 170 of about 553,332 (236)

GPCRs in CAR‐T Cell Immunotherapy: Expanding the Target Landscape and Enhancing Therapeutic Efficacy

Advanced Science, EarlyView.
Chimeric antigen receptor T cell therapy faces dual challenges of target scarcity and an immunosuppressive microenvironment in solid tumors. This review highlights how G protein‐coupled receptors can serve as both novel targets to expand the therapeutic scope and functional modules to enhance CAR‐T cell efficacy.
Zhuoqun Liu, Yuchen Xiao, Yamin Zhao, Lihe Dai, David J.H. Shih, Shikang Liang, Honglei Tian, Liu Liu, Feng Tian, Bing Liu, Lei Chang, Chao Zhang   +11 more
wiley   +1 more source

NanoLoop: A Deep Learning Framework Leveraging Nanopore Sequencing for Chromatin Loop Prediction

Advanced Science, EarlyView.
Chromatin loops are central to gene regulation and 3D genome organization. Leveraging Nanopore sequencing's ability to jointly capture DNA sequence and methylation, we present NanoLoop, the first framework for genome‐wide chromatin loop prediction using Nanopore data.
Wenjie Huang, Li Tang, Matthew C. Hill, Yonghao Zhang, Jun Xie, Min Li   +5 more
wiley   +1 more source

Integrated long-read transcriptomic profiling of peripheral blood from ankylosing spondylitis patients identifies regulatory shifts and core genes associated with programmed cell death. [PDF]

Front Immunol
Cao X, Li P, Peng H, Chen Q, Shi L, Chen D, Chu T, Cheng Y.   +7 more
europepmc   +1 more source

A Modular DNAzyme for Precise Visualization and Intervention of Alternative Splicing Isoforms in Live Cells

Advanced Science, EarlyView.
A Stringent dUPlex‐activated Error Robust (SUPER) DNAzyme system enables real‐time imaging of alternative mRNA splicing (e.g., Bcl‐xL/Bcl‐xS) in living cells via target‐triggered split‐DNAzyme reassembly and dual‐color fluorescence. It also achieves mRNA‐selective knockdown through DNAzyme‐based gene regulation, serving as a versatile tool for splicing
Mengru Lin, Jiale Sun, Yuqing Mao, Yuhao Tang, Fuan Wang, Zhihong Liu, Jing Wang   +6 more
wiley   +1 more source

Genetic Diagnosis and Discovery Enabled by Large Language Models

Advanced Science, EarlyView.
We demonstrate that large language models (LLMs) can facilitate genetic diagnosis and discovery. LLMs were used to solve four types of genetic problems of sequentially increased complexity. An LLM‐based pipeline could analyze genetic variants in the genomic sequences of human hearing loss or rare genetic disease patients and assist in identifying ...
Tao Tu, Khaled Saab, Weida Liu, Zhouqing Fang, Zhuanfen Cheng, Svetolik Spasic, Maja Djurisic, Hiroaki Mohri, Wenlong Ren, Anil Palepu, Juraj Gottweis, Alan Karthikesalingam, Kavita Kulkarni, Annalisa Pawlosky, Devon Bonner, Elijah Kravets, Shruti Marwaha, Hector R. Mendez, Matthew T. Wheeler, Jonathan A. Bernstein, Undiagnosed Diseases Network, Cheng‐Yu Tsai, Chen‐Chi Wu, Konstantina M. Stankovic, Vivek Natarajan, Gary Peltz   +25 more
wiley   +1 more source

Modified Polycyclic Compounds Rescue Mis-splicing in Myotonic Dystrophy Type 1 Disease Models. [PDF]

ACS Chem Biol
Frias JA, Hicks SM, Mazdiyasni H, Mishra SK, Sarkar K, Yeboah C, LeFever NM, Scotti MM, Zeghal H, Brandt N, Vangaveti S, Chakma P, Wang T, Reid TS, McMichael O, Crumbaugh C, Provenzano M, Hale MA, Cleary JD, Johnson NE, Wang ET, Reddy K, Berglund JA.   +22 more
europepmc   +1 more source

TGFβ1 Activates Lnc‐APUE to Promote Tumor Metastasis via the Alu Element‐Driven STAU1‐Mediated Decay of CDH1 mRNA

Advanced Science, EarlyView.
We delineate a TGFβ1/SMAD/lnc‐APUE/E‐cadherin axis wherein TGFβ1 signaling activates lnc‐APUE; lnc‐APUE subsequently downregulates E‐cadherin expression via Alu element‐driven STAU1‐mediated decay of CDH1 mRNA, thereby promoting tumor metastasis. Our findings reveal a novel regulatory function for lnc‐APUE within the SMD pathway, elucidate a distinct ...
Song‐Yang Li, Jia‐Hui Huang, Jin‐E Yang, Yi‐Hang Li, Juan‐Zhen Hong, Ting‐Ting Wang, Ying‐Lei Chi, Meng‐Zhi Wu, Wei Wang, Ying Zhu, Shi‐Mei Zhuang   +10 more
wiley   +1 more source

Landscape of splicing factors in early-onset gastric cancer reveals SRSF1 as a key driver of oxaliplatin resistance. [PDF]

J Biol Chem
Zhong B, Xiong Z, Xu H, Zheng J, Mohamed SA, Sun J, Huang D, Deng Z, Guo J, Peng J, Chen Y, Lian L.   +11 more
europepmc   +1 more source

Ascites circRNA ASCOR Drives Platinum Resistance of High‐Grade Serous Ovarian Cancer by Facilitating RPA1 Nuclear Translocation

Advanced Science, EarlyView.
High‐grade serous ovarian carcinoma (HGSOC) is plagued by platinum resistance, with malignant ascites closely linked to poor outcomes. Here, we identify ASCOR, a circRNA enriched in ascites small extracellular vesicles (sEVs) from platinum‐resistant HGSOC patients, as a predictor of poor survival. ASCOR enhances platinum resistance in vitro and in vivo
Hanyuan Liu, Chenchen Zhu, Xuelin Yao, Yi Liu, Jing Zhu, Yuebo Li, Hanjie Xu, Cheng Peng, Ge Shan, Liang Chen, Ying Zhou   +10 more
wiley   +1 more source

Elevated TRIM25 Impairs Poly (ADP‐ribose) Metabolism via PARG Degradation and Mediates Compression‐Induced Intervertebral Disc Degeneration

Advanced Science, EarlyView.
TRIM25 acts as a multifunctional hub driving intervertebral disc degeneration under mechanical stress. Mechanical compression significantly upregulates TRIM25 expression, establishing it as a key E3 ubiquitin ligase platform. TRIM25 targets PARG and Ku80 via distinct molecular interfaces, triggering their ubiquitination and degradation.
Zhangrong Cheng, Haiyang Gao, Wenbo Wu, Pengzhi Shi, Xianglong Chen, Zimu Yu, Wang Wu, Kangcheng Zhao, Cao Yang, Yukun Zhang   +9 more
wiley   +1 more source