Results 71 to 80 of about 225,555 (308)

Splicing Site Recognition by Synergy of Three Domains in Splicing Factor RBM10 [PDF]

Biochemistry, 2018
Splicing factor RBM10 and its close homologues RBM5 and RBM6 govern the splicing of oncogenes such as Fas, NUMB, and Bcl-X. The molecular architecture of these proteins includes zinc fingers (ZnFs) and RNA recognition motifs (RRMs). Three of these domains in RBM10 that constitute the RNA binding part of this splicing factor were found to individually ...
Pedro Serrano, John A. Hammond, Michael Geralt, Kurt Wüthrich   +3 more
openaire   +2 more sources

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

MYC-mediated transcriptional control of splicing factors promotes RNA splicing in activated B lymphocytes

Scientific Reports
Germinal center B cells play a pivotal role in the generation of long-lived plasma cells and high-affinity antibodies. Although RNA splicing and alternative splicing are known to regulate germinal center B cell responses, the upstream mechanisms ...
Silu Li, Chengyu Yang, Jiayuan Li, Zhenya Liu, Fenfang Gong, Xiaosu Ke, Gui-Xin Ruan, Hengjun Huang   +7 more
doaj   +1 more source

Regulation of RNA Splicing: Aberrant Splicing Regulation and Therapeutic Targets in Cancer

Cells, 2021
RNA splicing is a critical step in the maturation of precursor mRNA (pre-mRNA) by removing introns and exons. The combination of inclusion and exclusion of introns and exons in pre-mRNA can generate vast diversity in mature mRNA from a limited number of ...
Koji Kitamura, Keisuke Nimura
doaj   +1 more source

Disruption of pre-mRNA splicing in vivo results in reorganization of splicing factors [PDF]

The Journal of cell biology, 1994
We have examined the functional significance of the organization of pre-mRNA splicing factors in a speckled distribution in the mammalian cell nucleus. Upon microinjection into living cells of oligonucleotides or antibodies that inhibit pre-mRNA splicing in vitro, we observed major changes in the organization of splicing factors in vivo. Interchromatin
O'Keefe, Raymond T.; id_orcid 0000-0001-8764-1289, Mayeda, Akila, Sadowski, Cynthia L., Krainer, Adrian R., Spector, David L.   +4 more
openaire   +2 more sources

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

RNPS1 in PSAP complex controls periodic pre-mRNA splicing over the cell cycle

iScience
Summary: Cell cycle progression requires periodic gene expression through splicing control. However, the splicing factor that directly controls this cell cycle-dependent splicing remains unknown.
Kazuhiro Fukumura, Akio Masuda, Jun-ichi Takeda, Osamu Nagano, Hideyuki Saya, Kinji Ohno, Akila Mayeda   +6 more
doaj   +1 more source

Prognostic Signatures of Alternative Splicing Events in Esophageal Carcinoma Based on TCGA Splice-Seq Data

Frontiers in Oncology, 2021
An alternative splicing (AS) event is a highly complex process that plays an essential role in post-transcriptional gene expression. Several studies have suggested that abnormal AS events were the primary element in the pathological process of cancer ...
Ping Ye, Yan Yang, Liqiang Zhang, Guixi Zheng   +3 more
doaj   +1 more source

Post-transcriptional regulation of 5-lipoxygenase mRNA expression via alternative splicing and nonsense-mediated mRNA decay

, 2012
5-Lipoxygenase (5-LO) catalyzes the two initial steps in the biosynthesis of leukotrienes (LT), a group of inflammatory lipid mediators derived from arachidonic acid.
Laura Pufahl (182448), Manuel Grez, Grez, Manuel, Ochs, Meike J., Sorg, Bernd L., Beatrix Suess (17019), Sorg Bernd L., Laura Pufahl, Sorg, Bernd Lothar, Bernd L Sorg, Beatrix Suess, Bernd L. Sorg (182441), Suess Beatrix, Grez Manuel, Steinhilber Dieter, Dieter Steinhilber (182458), Meike J. Ochs (182432), Suess, Beatrix, Manuel Grez (182453), Dieter Steinhilber, Meike J Ochs, Pufahl, Laura, Süß, Beatrix, Pufahl Laura, Ochs, Meike, Steinhilber, Dieter, Ochs Meike J.   +26 more
core   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source