Results 91 to 100 of about 18,335 (219)

Detection of infectious versus inactivated sporozoites.

, 2013
Viability of cryopreserved sporozoites (A) and inactivation of heat-treated sporozoites (B) was confirmed by fluorescence microscopy (DeltaVision Core Microscope System with Coolsnap HQ camera and 100x/1.40 oil objective) using Sytox Blue (0.1% in PBS).
Simon H. Apte (140853), Sophie Schussek (465555), Penny L. Groves (140856), Denise L. Doolan (140876)   +3 more
core   +1 more source

Salivary gland-specific P. berghei reporter lines enable rapid evaluation of tissue-specific sporozoite loads in mosquitoes [PDF]

, 2012
Malaria is a life-threatening human infectious disease transmitted by mosquitoes. Levels of the salivary gland sporozoites (sgs), the only mosquito stage infectious to a mammalian host, represent an important cumulative index of <i>Plasmodium</i&
Levashina Elena A., Soichot, J., Blandine M Franke-Fayard, Annika Rademacher, Waters Andrew P., Chris J. Janse (12383), Elena A Levashina, Waters, A.P., Jai Ramesar (49002), Chandra Ramakrishnan, Ramakrishnan, C., Andrew P. Waters (15293), Soichot Julien, Costa, G., Costa Giulia, Ramakrishnan Chandra, Levashina, E.A., Giulia Costa (166412), Julien Soichot, Rademacher, A., Janse, C.J., Franke-Fayard Blandine M., Ramesar, J., Annika Rademacher (166404), Elena A. Levashina (160880), Franke-Fayard, B.M., Janse Chris J., Chris J Janse, Jai Ramesar, Rademacher Annika, Ramesar Jai, Blandine M. Franke-Fayard (166421), Giulia Costa, Julien Soichot (166407), Andrew P Waters, Chandra Ramakrishnan (134246)   +35 more
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The frequency and impact on the time to patency in humans of mosquito bites with ≤10 residual-sporozoites.

, 2017
Grey bars show how many volunteers received bites from mosquitoes with ≤10 salivary gland sporozoites. Black dots (and line) show model predictions for the mean time to patency for volunteers with different number of (≤10) bites.
Jamie T. Griffin (219983), Nick J. Edwards (151165), Ian D. Poulton (182342), Fiona Angrisano (132218), Andrew M. Blagborough (91736), Katarzyna A. Sala (224620), Robert E. Sinden (134250), Thomas S. Churcher (146323), Leanna M. Upton (224615), Patrick M. Brock (428558), Thomas W. Rampling (3634702), Sara E. Zakutansky (3634699), Adrian V. S. Hill (40512)   +12 more
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Serial Analysis of Gene Expression in salivary gland sporozoites [PDF]

, 2007
BACKGROUND: The invasion of Anopheles salivary glands by Plasmodium sporozoites is an essential step for transmission of the parasite to the vertebrate host. Salivary gland sporozoites undergo a developmental programme to express genes required for their
Bertrand Boisson, Ménard, Robert, Pierre Couble, Robert Ménard, Chertemps, Thomas, Paul Brey, Baldacci, Patricia, Emmanuel Bischoff, Brey, Paul, Thomas Chertemps, Patricia Baldacci, Sylvie Perrot, Rosinski-Chupin, Isabelle, Couble, Pierre, Bischoff, Emmanuel, Briolay, Jérôme, Jérôme Briolay, Boisson, Bertrand, Isabelle Rosinski-Chupin, Perrot, Sylvie   +19 more
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Expression of EaMIC5 protein in E. acervulina sporozoites by immunofluorescence assay (×100 magnification).

, 2014
Panel A: E. acervulina sporozoites. The white arrows indicate sporozoites. (A1) Differential interference contrast (DIC). (A2) Immunofluorescence localization using Cy3. (A3) DIC and Immunofluorescence combined.
LiXin Xu (3145221), ZhenChao Zhang (677810), MengHui Li (3755359), LianRui Liu (677813), XiangRui Li (3145224), XiaoKai Song (677815), Shuai Wang (109515), JingWei Huang (677811), YuXia Sui (677812), RuoFeng Yan (677814)   +9 more
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Plasmodium berghei rhoptry neck protein 6 maintains parasite infectivity and virulence

mBio
Apicomplexan parasites possess unique secretory organelles called rhoptries, which are reservoirs for rhoptry neck proteins (RONs) and rhoptry bulb proteins (ROPs) that aid in host cell attachment, invasion, and intracellular proliferation. In Plasmodium
Veeda Narahari, Smita Patri, Minal Dilip Satpute, Geeta Kashyap Vemuganti, Dilip Kumar Mishra, Surendra Kumar Kolli, Kota Arun Kumar   +6 more
doaj   +1 more source

Quantification of sporozoite expelling by Anopheles mosquitoes infected with laboratory and naturally circulating P. falciparum gametocytes

eLife
It is currently unknown whether all Plasmodium falciparum-infected mosquitoes are equally infectious. We assessed sporogonic development using cultured gametocytes in the Netherlands and naturally circulating strains in Burkina Faso.
Chiara Andolina, Wouter Graumans, Moussa Guelbeogo, Geert-Jan van Gemert, Jordache Ramijth, Soré Harouna, Zongo Soumanaba, Rianne Stoter, Marga Vegte-Bolmer, Martina Pangos, Photini Sinnis, Katharine Collins, Sarah G Staedke, Alfred B Tiono, Chris Drakeley, Kjerstin Lanke, Teun Bousema   +16 more
doaj   +1 more source

Quantitation of malaria sporozoites transmitted in vitro during salivation by wild Afrotropical Anopheles

, 1991
. The malaria transmission potential of wild, infective Anopheles from western Kenya was evaluated by determining the number of sporozoites transmitted in vitro by salivation when their mouthparts were inserted into capillary tubes containing either ...
MUTALIB RAMADHAN, WIRTZ, ROBERT A, DAVY K. KOECH, ROBERTS, CLIFFORD R, BEIER, JOHN C, KOROS, JOSEPH K, FRED K. ONYANGO, JOSEPH K. KOROS, RAMADHAN, MUTALIB, KOECH, DAVY K, OGWANG, ROSE, ROBERT A. WIRTZ, JOHN C. BEIER, ONYANGO, FRED K, CLIFFORD R. ROBERTS, ROSE OGWANG   +15 more
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Invasion capacity of Wt and Δp52 sporozoites in primary human hepatocytes in vitro.

, 2013
(A) Intra (In) and extracellular (Ex) sporozoites 3 hrs after incubation of sporozoites with primary human hepatocytes in culture. Sporozoites were first stained with anti-PfCSP antibodies (red). Then cells were permeabilised and sporozoites were stained
Audrey Gego (353873), Olivier Silvie (44628), Shahid M. Khan (190458), Stephen L. Hoffman (239213), Chris J. Janse (12383), Marga van de Vegte-Bolmer (359384), Samir Yalaoui (44627), Andrew P. Waters (15293), Jean-Francois Franetich (5653960), Dominique Mazier (44632), Robert W. Sauerwein (82233), Geert-Jan van Gemert (250563), Ben C. L. van Schaijk (236288), Melissa R. van Dijk (250560)   +13 more
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CS-RIImut Oocyst Sporozoites Are Not Released from the Midguts

, 2013
Represented in each graph are the mean numbers of sporozoites per mosquito at different days PI. Each number is calculated based on an average of 20 mosquitoes.(A) Numbers of oocyst sporozoites per mosquito from CS-WT– and CS-RIImut–infected mosquitoes ...
Qian Wang (32718), Hisashi Fujioka (59801), Victor Nussenzweig (59802)   +2 more
core   +1 more source