Phytopathology Research, 2023 Spray-induced gene silencing (SIGS) can inhibit plant diseases by topical application of double- (dsRNA) or single-stranded (sRNA) RNA molecules onto plants to silence virulence-related pathogen genes. However, the on-field application of SIGS is limited
Yumeng Wang +6 more
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Small RNA-based plant protection against diseases
Frontiers in Plant Science, 2022 Plant diseases cause significant decreases in yield and quality of crops and consequently pose a very substantial threat to food security. In the continuous search for environmentally friendly crop protection, exploitation of RNA interferance machinery ...
Özlem Bilir +5 more
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Factors influencing Barley Stripe Mosaic Virus-mediated gene silencing in wheat [PDF]
, 2010 Virus induced gene silencing (VIGS) is a technology that has been used primarily to target the virus genome in infected plants. However, if the virus genome carries inserts derived from the host plant, the system could be employed to target the mRNAs ...
Cakir, Cahid +5 more
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RNA interference is ineffective as a routine method for gene silencing in chick embryos as monitored by fgf8 silencing. [PDF]
, 2013 The in vivo accessibility of the chick embryo makes it a favoured model system for experimental developmental biology. Although the range of available techniques now extends to miss-expression of genes through in ovo electroporation, it remains difficult
Bueno i Torrens, David, 1965- +1 more
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Internuclear gene silencing in Phytophthora infestans is established through chromatin remodelling [PDF]
, 2008 In the plant pathogen Phytophthora infestans, nuclear integration of inf1 transgenic DNA sequences results in internuclear gene silencing of inf1. Although silencing is regulated at the transcriptional level, it also affects transcription from other ...
Li, S. +7 more
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RNAi spray-induced gene silencing of <i>EPSPS</i> by topical application of dsRNA in the weed <i>Digitaria insularis</i>. [PDF]
Front Plant SciThe expanding cultivation of grains to meet agro-industry demands, the implementation of more efficient, sustainable, and integrative management practices capable of mitigating the selection of resistant pests and weeds becomes necessary. Due to the intensive application of chemical herbicides, in addition to environmental impacts,
Cintra LA +7 more
europepmc +3 more sources
Catch Me If You Can! RNA Silencing-Based Improvement of Antiviral Plant Immunity
Viruses, 2019 Viruses are obligate parasites which cause a range of severe plant diseases that affect farm productivity around the world, resulting in immense annual losses of yield.
Fatima Yousif Gaffar, Aline Koch
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Potato virus X-induced gene silencing in leaves and tubers of potato [PDF]
, 2004 Virus induced gene silencing (VIGS) is increasingly used to generate transient loss-of-function assays and has potential as a powerful reverse-genetics tool in functional genomic programs as a more rapid alternative to stable transformation. A previously
Loake, Gary J +17 more
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Integrating RNAi Technology in Smallholder Farming: Accelerating Sustainable Development Goals
Frontiers in Sustainable Food Systems, 2022 Approximately 84% of farms globally are <2 hectares; these and other smallholder farms collectively produce over one third of humanity's food. However, smallholder farms, particularly in developing countries, encounter difficulties in both production ...
Jonathan Willow +2 more
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The role of TNRC6 proteins in gene silencing [PDF]
, 2013 Proteins of the GW182 family have recently emerged as key players in miRNAmediated gene silencing. They have been shown to interact with Argonaute proteins, components of the RISC and are assumed to mediate the repression in metazoa. Three paralogues are
Zipprich, Jakob Theophil
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