Rational dsRNA design, scalable production and nanodelivery to enhance spray-induced gene silencing [PDF]
Communications BiologySpray-induced gene silencing (SIGS) provides a sustainable, highly targeted alternative to chemical pesticides. This review summarizes recent advances in dsRNA technology through a design-to-delivery framework.
Guoning Zhu, Chao Xu, Tao Zhang
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Enhancing Fusarium resistance in Nigella sativa via spray-induced gene silencing (SIGS) using chitosan nanoparticles with molecular and phytochemical perspectives [PDF]
Scientific ReportsFusarium oxysporum is the causal agent of vascular wilt disease, which makes it hard to grow and use Nigella sativa as medicine. This disease significantly reduces both yield and phytochemical quality. Traditional methods of control frequently utilize chemical fungicides; however, these can leave behind harmful residues and modify the bioactive profile
Rouya Mohammed Ahmed +9 more
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Spray-Induced Gene Silencing (SIGS) of Dual-Target Genes (AaGH10 and AaSOD) Enhances Control of Alternaria alternata White Leaf Spot in Chinese Chive [PDF]
Journal of FungiChinese chive (Allium tuberosum) suffers severe yield and quality losses from white leaf spot disease caused by Alternaria alternata. Spray-induced gene silencing (SIGS) presents a sustainable alternative to traditional chemical fungicides. To maximize the biocontrol efficacy of this approach, we designed dsRNAs targeting two candidate virulence ...
Lianzhe Wang +10 more
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RNA Interference and Spray-Induced Gene Silencing (SIGS): A Next-Generation Tool in Horticultural Biotechnology [PDF]
BIO Web of ConferencesRNA interference (RNAi) is a conserved post-transcriptional gene silencing mechanism that enables sequence-specific regulation of gene expression through double-stranded RNA (dsRNA). In horticultural systems, it has emerged as a very novel and innovative
Dixit Nishtha +3 more
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Frontiers in Plant Science, 2021 Over the past years, RNA interference (RNAi) has been used as a promising combat strategy against a wide range of pests and pathogens in ensuring global food security.
Atrayee Sarkar, Subhankar Roy-Barman
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Frontiers in Plant Science, 2020 Over the last decade, several studies have revealed the enormous potential of RNA-silencing strategies as a potential alternative to conventional pesticides for plant protection.
Bernhard Timo Werner +4 more
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Express delivery: dsRNA applications and RNAi nanocarrier systems to combat potato late blight [PDF]
Frontiers in Plant SciencePhytophthora late blight remains one of the most devastating diseases of potatoes, posing persistent challenges to global food security and sustainable agriculture.
Ellen Vandenbussche +3 more
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Spray-induced gene silencing for plant disease control: mechanistic basis and deployment-oriented design [PDF]
Frontiers in Plant ScienceSpray-induced gene silencing (SIGS) is a transgene-free, biodegradable RNA-based approach for crop protection in which exogenously applied double-stranded RNA (dsRNA) is taken up and processed by plants or susceptible pathogens to drive the sequence ...
Mohamed Mannaa +5 more
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RNAi‐Based Pesticides: Genetic Innovations for Sustainable Crop Protection and One Health [PDF]
Advanced GeneticsConventional chemical pesticides have long protected global crop yields, but their environmental and health impacts, including contamination, biodiversity loss, residue accumulation, and pest resistance, demand sustainable alternatives. RNA interference (
Jisheng Liu, Guy Smagghe
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Recent Advances in Pathogenicity and Biocontrol of Postharvest Penicillium Diseases [PDF]
Journal of FungiPenicillium species are major postharvest pathogens of fruits and vegetables, causing significant economic losses and posing serious threats to food safety through mycotoxin contamination.
Guohua Yin +4 more
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