Rational dsRNA design, scalable production and nanodelivery to enhance spray-induced gene silencing [PDF]
Communications BiologySpray-induced gene silencing (SIGS) provides a sustainable, highly targeted alternative to chemical pesticides. This review summarizes recent advances in dsRNA technology through a design-to-delivery framework.
Guoning Zhu, Chao Xu, Tao Zhang
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Control Strategies of Plant Viruses Using Spray-Induced Gene Silencing [PDF]
The Plant Pathology JournalPlant viruses severely limit global crop productivity, yet no pesticide-like antiviral agents are currently available for effective control. Double-stranded RNA (dsRNA) technologies, acting through RNA interference, provide a sequence-specific and non ...
Seung-Kook Choi +4 more
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The impact of spray-induced gene silencing on cereal phyllosphere microbiota [PDF]
Environmental MicrobiomeBackground Fusarium head blight (FHB) is a major disease affecting cereal crops including wheat, barley, rye, oats and maize. Its predominant causal agent is the ascomycete fungus Fusarium graminearum, which infects the spikes and thereby reduces grain ...
Poorva Sundararajan +6 more
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Gene Mining for Conserved, Non-Annotated Proteins of Podosphaera xanthii Identifies Novel Target Candidates for Controlling Powdery Mildews by Spray-Induced Gene Silencing [PDF]
Journal of Fungi, 2021 The powdery mildew fungus Podosphaera xanthii is one of the most important limiting factors for cucurbit production worldwide. Despite the significant efforts made by breeding and chemical companies, effective control of this pathogen remains elusive to ...
Laura Ruiz-Jiménez +4 more
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Spray-induced gene silencing for crop protection: recent advances and emerging trends [PDF]
Frontiers in Plant ScienceThe RNA-based spray-induced gene silencing (SIGS) technology represents an ecologically sustainable approach to crop protection and pathogen management.
Can Chen +4 more
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Phyllosphere microbiome responses to spray-induced gene silencing targeting Phytophthora infestans in potato [PDF]
npj Biofilms and MicrobiomesRecent years have seen a growing interest in the use of RNA interference to control filamentous pathogens, creating a new niche in plant disease management.
Samrat Ghosh +7 more
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Spray-induced gene silencing for plant disease control: mechanistic basis and deployment-oriented design [PDF]
Frontiers in Plant ScienceSpray-induced gene silencing (SIGS) is a transgene-free, biodegradable RNA-based approach for crop protection in which exogenously applied double-stranded RNA (dsRNA) is taken up and processed by plants or susceptible pathogens to drive the sequence ...
Mohamed Mannaa +5 more
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Frontiers in Plant Science, 2021 Over the past years, RNA interference (RNAi) has been used as a promising combat strategy against a wide range of pests and pathogens in ensuring global food security.
Atrayee Sarkar, Subhankar Roy-Barman
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Spray-induced gene silencing enables the characterization of gene function during pre-penetration stages in Blumeria graminis f. sp. tritici [PDF]
Frontiers in Plant ScienceBlumeria graminis f. sp. tritici (Bgt), the causal agent of wheat powdery mildew, poses a significant threat to global wheat production. As an obligate biotroph, Bgt is recalcitrant to stable genetic manipulation. Although host-induced gene silencing has
Meihui Zhang +11 more
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Frontiers in Plant Science, 2020 Over the last decade, several studies have revealed the enormous potential of RNA-silencing strategies as a potential alternative to conventional pesticides for plant protection.
Bernhard Timo Werner +4 more
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