Inositol pyrophosphates activate the vacuolar transport chaperone complex in yeast by disrupting a homotypic SPX domain interaction [PDF]
Nature Communications, 2023 Many proteins involved in eukaryotic phosphate homeostasis are regulated by SPX domains. In yeast, the vacuolar transporter chaperone (VTC) complex contains two such domains, but mechanistic details of its regulation are not well understood.
Joka Pipercevic +10 more
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Inositol pyrophosphate dynamics reveals control of the yeast phosphate starvation program through 1,5-IP8 and the SPX domain of Pho81 [PDF]
eLife, 2023 Eukaryotic cells control inorganic phosphate to balance its role as essential macronutrient with its negative bioenergetic impact on reactions liberating phosphate.
Valentin Chabert +7 more
doaj +7 more sources
Inositol pyrophosphates promote the interaction of SPX domains with the coiled-coil motif of PHR transcription factors to regulate plant phosphate homeostasis [PDF]
Nature Communications, 2021 Plants regulate phosphate homeostasis via the interaction of PHR transcription factors with SPX receptors bound to inositol pyrophosphate signaling molecules.
Martina K. Ried +11 more
doaj +11 more sources
Genome-wide identification and characterization of SPX-domain-containing protein gene family in Solanum lycopersicum [PDF]
PeerJ, 2021 The SYG1, PHO81, and XPR1 (SPX) domain is named after the suppressor of yeast gpa1 (Syg1), yeast phosphatase (Pho81) and the human Xenotropic and Polytrophic Retrovirus receptor1 (XPR1).
Chunwei Li, Qiuye You, Panfeng Zhao
doaj +5 more sources
Cleavage-Polyadenylation Factor Cft1 and SPX Domain Proteins Are Agents of Inositol Pyrophosphate Toxicosis in Fission Yeast [PDF]
mBio, 2022 Inositol pyrophosphate (IPP) dynamics govern expression of the fission yeast phosphate homeostasis regulon via their effects on lncRNA-mediated transcription interference.
Beate Schwer +5 more
doaj +4 more sources
IP7-SPX Domain Interaction Controls Fungal Virulence by Stabilizing Phosphate Signaling Machinery [PDF]
mBio, 2020 In the human-pathogenic fungus Cryptococcus neoformans, the inositol polyphosphate signaling pathway is critical for virulence. We recently demonstrated the key role of the inositol pyrophosphate IP7 (isomer 5-PP-IP5) in driving fungal virulence; however,
Desmarini Desmarini +11 more
doaj +5 more sources
Widespread Presence of SPX and Its Potential Role as a Phosphorus Nutrient Regulator in Dinoflagellates [PDF]
MicroorganismsSPX domain-containing proteins (SPXc) are crucial for regulating phosphorus (P) homeostasis in plants. Recently, the SPX gene was identified in the diatom model Phaeodactylum tricornutum and shown to serve as a negative regulator of P acquisition ...
Jiashun Li +4 more
doaj +3 more sources
Comparative analysis of seven types of phosphate transporters in forty species from the phylogenetic and transcriptomic perspective. [PDF]
PLoS ONEAcquisition of phosphorus (P), an essential macronutrient for plants, is a limiting factor in most soils. Phosphate transporter proteins (PTs) play an indispensable role in inorganic phosphate (Pi) transport across the membrane barriers in plants.
Junyi Ren +5 more
doaj +2 more sources
The intracellular inositol (pyro)phosphate receptor AtSPX1 reciprocally binds to P1BS DNA [PDF]
Nature CommunicationsThe response of plants to phosphate starvation engages PHR (CC-MYB-PHOSPHATE STARVATION RESPONSE) transcription factors that bind to P1BS (GNATATNC) promoter elements of phosphate-starvation induced (PSI) genes. The encoded proteins include single-domain
Hayley L. Whitfield +6 more
doaj +2 more sources
Structural insights into the gating mechanism of the fission yeast phosphate exporter SpXpr1 [PDF]
Cell DiscoveryPhosphate homeostasis is essential for fundamental cellular processes, including energy metabolism, signal transduction, and nucleic acid synthesis. Although XPR1 family proteins are conserved phosphate exporters throughout eukaryotes, their structural ...
Hui Yang +6 more
doaj +2 more sources