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Benefits of stable isotope labeling in RNA analysis
Biological Chemistry, 2019AbstractRNAs are key players in life as they connect the genetic code (DNA) with all cellular processes dominated by proteins. They contain a variety of chemical modifications and many RNAs fold into complex structures. Here, we review recent progress in the analysis of RNA modification and structure on the basis of stable isotope labeling techniques ...
Carine Tisné +2 more
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Stable Isotope Labeling in Mammals (SILAM)
Cold Spring Harbor Protocols, 2008INTRODUCTIONA general approach in quantitative mass spectrometry is to mix a protein sample containing only natural-abundance isotopes with an identical protein sample containing proteins labeled with heavy stable isotopes (e.g., 2H, 13C, 15N, or 18O).
Daniel B, McClatchy, John R, Yates
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Dynamic lipidomics with stable isotope labelling
Journal of Chromatography B, 2009Incorporation of stable isotope labelled precursors enables estimation of the kinetics of lipid synthesis and turnover (dynamic lipidomics) in the clinical as well the experimental setting. Recent advances in tandem mass spectrometry extend the analytical possibilities from measurements of isotope enrichments to determinations of intact substrates ...
Postle, Anthony D., Hunt, Alan N.
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Stable Isotopic Labeling for Proteomics
2011Since the coining of the term Proteome, the field of Proteomics has developed rapidly and come to rely heavily on mass spectrometry, initially for protein identification but recently the use of stable isotopic labels for protein quantitation has grown in importance.
Ashman, Keith +3 more
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Labeling Monosaccharides With Stable Isotopes
2015Chemical and chemi-enzymic methods are discussed for the preparation of monosaccharide isotopomers that are singly and multiply labeled with (13)C, (2)H, (17/18)O, and (15)N isotopes. The discussion focuses primarily on chemical methods to incorporate stable isotopes into monosaccharides and not on methods to assemble labeled monosaccharides into more ...
Wenhui, Zhang +2 more
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Journal of Labelled Compounds and Radiopharmaceuticals, 2017
Two regioisomeric glucuronide metabolites of dapagliflozin (BMS‐512148) were synthesized and used to elucidate the structures of dapagliflozin metabolites observed in human urine samples. The structures of the synthetic metabolites were assigned by heteronuclear multiple‐bond correlation, ROESY, and total correlation spectroscopy experiments. Analogues
Kai, Cao +5 more
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Two regioisomeric glucuronide metabolites of dapagliflozin (BMS‐512148) were synthesized and used to elucidate the structures of dapagliflozin metabolites observed in human urine samples. The structures of the synthetic metabolites were assigned by heteronuclear multiple‐bond correlation, ROESY, and total correlation spectroscopy experiments. Analogues
Kai, Cao +5 more
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Distribution and metabolism of selenohomolanthionine labeled with a stable isotope
Analytical and Bioanalytical Chemistry, 2010The distribution and metabolism of selenohomolanthionine (4,4'-selenobis[2-aminobutanoic acid], SeHLan), a newly identified selenoamino acid in selenized Japanese pungent radish, were evaluated by administering ⁷⁷Se-labeled SeHLan at a dose of 25 μg/kg body weight in rats.
Yasumi, Anan +3 more
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Synthesis of carbon‐14 and stable isotope labeled Censavudine
Journal of Labelled Compounds and Radiopharmaceuticals, 2022Censavudine is a nucleoside reverse transcriptase inhibitor (NRTI) explored clinically by Bristol Myers Squibb for the treatment of human immunodeficiency virus‐1 (HIV‐1). As part of the development process, a carbon‐14 labeled analog was synthesized for use in a human absorption, distribution, metabolism, and excretion (ADME) study.
Richard C. Burrell +4 more
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Stable-Isotope Dimethyl Labeling for Quantitative Proteomics
Analytical Chemistry, 2003In this paper, we report a novel, stable-isotope labeling strategy for quantitative proteomics that uses a simple reagent, formaldehyde, to globally label the N-terminus and epsilon-amino group of Lys through reductive amination. This labeling strategy produces peaks differing by 28 mass units for each derivatized site relative to its nonderivatized ...
Jue-Liang, Hsu +3 more
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Synthesis of stable isotope labeled 3-acetyldeoxynivalenol
Molecular Nutrition & Food Research, 2005Stable isotope labeled 3-acetyldeoxynivalenol (3-AcDON) was synthesized in excellent yield from deoxynivalenol as starting material. This is the first synthesis of a stable isotope labeled type-B trichothecene suitable as internal standard for HPLC-MS/MS or GC-MS analysis of trichothecene mycotoxins.
Michael, Bretz +3 more
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