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Stable Isotope Labeling Methods in Protein Profiling
2013Mass spectrometry (MS) analysis of peptides and proteins has evolved dramatically over the last 20 years. Improvement of MS instrumentation, computational data analysis, and the availability of complete sequence databases for many species have made large-scale proteomics analyses possible.
Lengqvist J, Sandberg A
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Stable isotope labeled alpha‐ketoacids
Journal of Labelled Compounds and Radiopharmaceuticals, 1984AbstractAlkylation of diethyl oxalpropionate with [D3]methyl iodide followed by acid hydrolysis led to 3‐[D3]methyl‐2‐oxobutyric (α‐ketoisovaleric) acid. Alkylation of N‐t‐butylpropylimine with [D3]methyl iodide followed by hydrolysis gave 2‐[D3]methylpropanal. This was further alkylated with 2,2‐difluoro‐1‐tosyloxyvinyllithium and hydrolyzed to afford
Sun‐Shine Yuan, Alfred M. Ajami
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Stable isotopic labelling of heterocyclic compounds
Journal of Labelled Compounds and Radiopharmaceuticals, 2007AbstractStable isotopically labelled (SIL) versions of Glimepiride 1 (10 steps, 11% overall yield), a blood glucose lowering drug, and Melagatran 13 (9 steps, 17% overall yield), an anticoagulant with similar uses to warfarin, were synthesized as internal standards for LCMS assays. Modifications of known routes (Weyer et al., US Patent 4379785, Hocchst,
Andrew J. Burton, Alan H. Wadsworth
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Applications of stable isotope dimethyl labeling in quantitative proteomics
Analytical and Bioanalytical Chemistry, 2012 Mass spectrometry has proven to be an indispensable tool for protein identification, characterization, and quantification. Among the possible methods in quantitative proteomics, stable isotope labeling by using reductive dimethylation has emerged as a cost-effective, simple, but powerful method able to compete at any level with the present alternatives.
Duangnapa Kovanich +2 more
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Metabolism of selenite labelled with enriched stable isotope in the bloodstream
Journal of Chromatography B: Biomedical Sciences and Applications, 1997The metabolism of selenium (Se) in the bloodstream of rats was studied using HPLC-ICP-MS with an enriched Se stable isotope, and the results were used as Se-specific indicators for Se nutritional status. Concentration of endogenous Se in plasma depended on dietary Se, while changes in concentrations and distributions of exogenous Se revealed its ...
K T, Suzuki, M, Itoh
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Stable Isotope Labeling and Quantification of Photosynthetic Metabolites
Stable isotope labeling with 13CO2 coupled with mass spectrometry allows monitoring the incorporation of 13C into photosynthetic intermediates and is a powerful technique for the investigation of the metabolic dynamics of photosynthesis. We describe here a protocol for 13CO2 labeling of large leaved plants and of Arabidopsis thaliana rosette, and a ...Chiara Baccolini, Stéphanie Arrivault
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Synthesis of stable isotope labeled norepinephrine
Journal of Labelled Compounds, 1975AbstractRacemic norepinephrine was synthesized with three deuterium atoms on the alkyl chain. The deuteration was accomplished by D/H exchange on the intermediate, dibenzylaminodihydroxyacetophenone, followed by reduction of the keto moiety and cleavage of the benzyl‐protecting groups with deuterium gas.
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Progress in stable isotope labeled quantitative proteomics methods
Chinese Journal of Chromatography, 2013Quantitative proteomics is an important research field in post-genomics era. There are two strategies for proteome quantification: label-free methods and stable isotope labeling methods which have become the most important strategy for quantitative proteomics at present. In the past few years, a number of quantitative methods have been developed, which
Yuan, Zhou +3 more
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Stable isotope labeling of sugars simplified
Chemical & Engineering News Archive, 1979A new way to label sugar molecules with stable atomic isotopes in practically any position has been developed by biochemists at Michigan State University, East Lansing. Their contribution, part of a national program to exploit stable atomic isotopes, will help spread the use of methods, such as nuclear magnetic resonance spectroscopy and mass ...
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Stable Isotope Labeling for Proteomic Analysis of Tissues in Mouse
2014Since the first metabolic labeling experiments with stable isotopes beginning of the last century, several approaches were pursued to monitor protein dynamics in living animals. Today, almost all model organisms from bacteria to rodents can be fully labeled with SILAC (stable isotope labeling of amino acids in cell culture) amino acids. The development
Soraya, Hölper +2 more
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